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- 规格：20μl质粒
- 质粒图谱1： png/1-1F92F911202T.png
质粒序列： 点击查看序列LOCUS Exported 6159 bp ds-DNA circular SYN 27-SEP-2017DEFINITION synthetic circular DNAACCESSION .VERSION .KEYWORDS .SOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 6159)AUTHORS .TITLE Direct SubmissionJOURNAL Exported Sep 27, 2017 from MiaoLingPlasmidhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..6159/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 235..614/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 615..818/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 863..881/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"misc_feature 889..976/label=MCSCDS 977..1696/codon_start=1/product="enhanced GFP"/label=EGFP/note="mammalian codon-optimized"/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"promoter complement(1712..1730)/label=SP6 promoter/note="promoter for bacteriophage SP6 RNA polymerase"polyA_signal 1756..1980/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin 2026..2454/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 2468..2797/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 2648..2783/label=SV40 ori/note="SV40 origin of replication"CDS 2864..3658/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Neo/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 3832..3953/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(4404..4992)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(5163..6023)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(6024..6128)/gene="bla"/label=AmpR promoterORIGIN1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca961 ctggcggccg ctcgagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat1021 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga1081 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc1141 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta1201 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca1261 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt1321 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg1381 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc1441 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg1501 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct1561 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa1621 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga1681 cgagctgtac aagtaatcta gagggcccta ttctatagtg tcacctaaat gctagagctc1741 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg1801 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa1861 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca1921 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg1981 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg2041 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg2101 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc2161 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc2221 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga2281 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa2341 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga2401 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct2461 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat2521 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc2581 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac2641 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact2701 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta2761 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc2821 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg2881 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca2941 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt3001 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg3061 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga3121 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca3181 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct3241 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac3301 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc3361 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt3421 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt3481 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg3541 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat3601 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc3661 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc3721 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc3781 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt3841 attgcagctt ataatggtta caa
