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- 规格：20μl质粒
- 启动子：T7/lac promoter
- 终止子：T7 terminator
- 质粒大小：5634bp
- 质粒标签：N-Strep-Tag II，N-HRV 3C site
- 原核抗性：壮观霉素Spe
- 克隆菌株：大肠杆菌Stbl3
- 质粒简介： pWUR702质粒可诱导表达TtAgo蛋白，在N端携带有Strep-Tag II标签和HRV 3C site蛋白酶酶切位点。本质粒参考文献为：DNA-guided DNA interference by a prokaryotic Argonaute.
- 质粒图谱1： png/1-1G025161K5921.png
质粒序列： 点击查看序列LOCUS Exported 5634 bp ds-DNA circular SYN 25-OCT-2017DEFINITION synthetic circular DNASOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 5634)TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..5634/organism="synthetic DNA construct"/mol_type="other DNA"protein_bind 3..27/label=lac operator/bound_moiety="lac repressor encoded by lacI"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."CDS 80..103/codon_start=1/product="peptide that binds Strep-Tactin(R), an engineeredform of streptavidin"/label=Strep-Tag II/translation="WSHPQFEK"CDS 110..133/codon_start=1/product="recognition and cleavage site for humanrhinovirus 3C and PreScission proteases"/label=HRV 3C site/translation="LEVLFQGP"misc_feature 141..2200/label=TtAgo/note="Thermus thermophilus TTHB068 AP008227 "terminator 2315..2362/label=T7 terminator/note="transcription terminator for bacteriophage T7 RNApolymerase"CDS complement(2533..3324)/codon_start=1/gene="aadA"/product="aminoglycoside adenylyltransferase (Murphy,1985)"/label=Spe/note="confers resistance to spectinomycin andstreptomycin"/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"promoter complement(3325..3416)/gene="bla"/label=AmpR promoterrep_origin complement(3464..4202)/direction=LEFT/label=CloDF13 ori/note="Plasmids containing the CloDF13 (CDF) origin ofreplication can be propagated in E. coli cells that containadditional plasmids with compatible origins."CDS complement(4412..5494)/codon_start=1/gene="lacI"/product="lac repressor"/label=lacI/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"promoter complement(5495..5572)/gene="lacI"/label=lacI promotermisc_feature 5618..2/label=T7 promoterORIGIN1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag61 gagatatacc atgtcaagct ggagccaccc gcagttcgaa aagggtgcac ttgaagtcct121 ctttcaggga cccgggtacc agatgaacca ccttggaaaa acggaagtgt tcctaaaccg181 cttcgcccta cggcctctga accccgagga actcaggcct tggcgcctcg aggtggttct241 ggaccctcct ccggggcggg aggaggtgta tccccttctc gcccaggtgg cccgccgggc301 ggggggcgtc acggtgcgca tgggagacgg cctcgcctcc tggtcacccc ctgaggtcct361 ggtcttggag ggcaccttgg cgcggatggg gcagaactac gcctaccgcc tctaccccaa421 ggggaggagg cctctggacc ccaaggaccc gggggagcgg agcgtgcttt cggccctagc481 ccgaaggctc cttcaggagc gcctcaggcg cctcgagggg gtctgggtgg aggggcttgc541 ggtgtaccgg agggagcacg cccgggggcc cgggtggcgg gtgcttgggg gggcggtctt601 ggacctttgg gtctccgact cgggggcgtt cctcctggag gtggaccccg cttaccgcat661 cctctgcgag atgtccctcg aggcctggct tgcccaaggc caccctctgc ccaaacgggt721 ccggaacgcc tacgaccggc gcacctggga gcttctccgg ctgggagagg aagaccccaa781 ggagctccct ctcccgggcg gcttgagcct cctggactac cacgctttca agggccgcct841 ccagggccgg gaaggggggc gggtggcctg ggtggcggac ccaaaggatc cgcgaaagcc901 aatcccccat ctgacgggcc ttttggtgcc cgttctgacc ctggaagacc ttcatgaaga961 ggagggaagc ctggccctct ccttgccttg ggaagagcgg cgtcggcgga cccgggagat1021 cgccagctgg atcggccggc gcctggggct gggcacacct gaggcggtgc gcgcccaggc1081 ttaccgcttg agcatcccca agctcatggg cagaagagcc gtgagcaagc ctgcggacgc1141 cctccgcgtg gggttttacc gggcccagga aaccgccctg gccctcttgc gcctggacgg1201 ggcccaagga tggccggagt ttctccggcg ggccttgctc cgggcctttg gggcgagcgg1261 ggcttccctc cgtttgcaca ccctccacgc ccatccctcc caaggcctgg ctttccgcga1321 ggcgctgcgg aaggccaagg aggagggggt ccaggccgtg ctggtcctca ccccgcccat1381 ggcctgggaa gaccgcaacc gcctgaaggc ccttcttctc agggaaggcc ttcccagcca1441 aatcctcaac gtccccctcc gggaggagga acgccaccgc tgggagaacg ccctcctggg1501 cctcctggcc aaagcggggc ttcaggtggt ggccctgagc ggcgcctatc cggcggagct1561 cgccgtgggc tttgacgccg gcggaaggga gtcctttcgc ttcgggggcg cggcctgcgc1621 cgtgggcggg gacggcggcc acctcctctg gaccctcccc gaggcccagg ccggggagcg1681 gatcccccag gaggtggtct gggacctctt ggaggagacc ctctgggcct tcagacgcaa1741 ggcggggagg cttccttccc gggtcctcct ccttcgggac ggccgcgtgc cccaggacga1801 gttcgccctg gccttggagg cccttgcccg ggaaggcata gcctacgact tggtttcggt1861 gcgcaagtcg ggtggggggc gggtctaccc cgtgcagggg cgcctggcgg acgggcttta1921 cgtccccttg gaggacaaga cctttcttct ccttaccgtc caccgggact tccggggcac1981 gccccgaccc ctgaagctgg tgcacgaggc gggggacacg cccctcgagg ccctggccca2041 ccagattttc catctgaccc gcctctaccc ggcgagcggt ttcgccttcc cccggcttcc2101 cgctcccctt cacctggccg accgcctggt gaaggaggtg ggccggttgg ggatccgtca2161 cctcaaggag gtggaccggg aaaagctctt cttcgtttag gaattcgcgg ccgcagagct2221 cgctctggtg ccacgcggta gttccgctca tcaccaccat catcaccatc accaccacta2281 attaacctag gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa2341 acgggtcttg aggggttttt tgctgaaacc tcaggcattt gagaagcaca cggtcacact2401 gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc2461 ctgccctgaa ccgacgaccg ggtcatcgtg gccggatctt gcggcccctc ggcttgaacg2521 aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa2581 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg2641 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc2701 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt2761 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt2821 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc2881 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat2941 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa3001 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt3061 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc3121 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc3181 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag3241 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac3301 ttcggcgatc accgcttccc tcatactctt cctttttcaa tattattgaa gcatttatca3361 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagc3421 tagctcactc ggtcgctacg ctccgggcgt gagactgcgg cgggcgctgc ggacacatac3481 aaagttaccc acagattccg tggataagca ggggactaac atgtgaggca aaacagcagg3541 gccgcgccgg tggcgttttt ccataggctc cgccctcctg ccagagttca cataaacaga3601 cgcttttccg gtgcatctgt gggagccgtg aggctcaacc atgaatctga cagtacgggc3661 gaaacccgac aggacttaaa gatccccacc gtttccggcg ggtcgctccc tcttgcgctc3721 tcctgttccg accctgccgt ttaccggata cctgttccgc ctttctccct tacgggaagt3781 gtggcgcttt ctcatagctc acacactggt atctcggctc ggtgtaggtc gttcgctcca3841 agctgggctg taagcaagaa ctccccgttc agcccgactg ctgcgcctta tccggtaact3901 gttcacttga gtccaacccg gaaaagcacg gtaaaacgcc actggcagca gccattggta3961 actgggagtt cgcagaggat ttgtttagct aaacacgcgg ttgctcttga agtgtgcgcc4021 aaagtccggc tacactggaa ggacagattt ggttgctgtg ctctgcgaaa gccagttacc4081 acggttaagc agttccccaa ctgacttaac cttcgatcaa accacctccc caggtggttt4141 tttcgtttac agggcaaaag attacgcgca gaaaaaaagg atctcaagaa gatcctttga4201 tcttttctac tgaaccgctc tagatttcag tgcaatttat ctcttcaaat gtagcacctg4261 aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgcccc gcgcccaccg4321 gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg
