• 规格：20μl质粒
• 质粒大小：3288bp
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：               pDC312质粒是一个腺病毒表达载体，MCS处前后无CMV启动子和poly A终止子，方便插入完整表达盒。
• 质粒图谱1： png/1-1G025155010L9.png

质粒序列： 点击查看序列

LOCUS       Exported                3288 bp ds-DNA     circular SYN 25-OCT-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 3288)

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..3288

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_signal     190..340

                     /label=Ad5 Psi

                     /note="packaging signal for adenovirus serotype 5"

     misc_feature    455..508

                     /label=MCS

     protein_bind    526..559

                     /label=loxP

                     /bound_moiety="Cre recombinase"

                     /note="Cre-mediated recombination occurs in the 8-bp core 

                     sequence (GCATACAT)."

     rep_origin      complement(1022..1610)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(1781..2641)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(2642..2746)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt

       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg

      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg

      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt

      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa

      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg

      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc

      421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gtcgacgagc tcaagcttag

      481 atctgctagc aggcctgaat tcggatccct cgactcgaag atccaataac ttcgtatagc

      541 atacattata cgaagttata agtgccgccc tataccttgt ctgcctcccc gcgttgcgtc

      601 gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg

      661 attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac

      721 caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat

      781 ctcggctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct

      841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat

      901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga

      961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt

     1021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt

     1081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc

     1141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa

     1201 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct

     1261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta

     1321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg

     1381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc

     1441 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta

     1501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg

     1561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt

     1621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg

     1681 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta

     1741 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg

     1801 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg

     1861 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc

     1921 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg

     1981 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg

     2041 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag

     2101 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat

     2161 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc

     2221 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc

     2281 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa

     2341 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac

     2401 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt

     2461 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc

     2521 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa

     2581 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca

     2641 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat

     2701 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa

     2761 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc

     2821 gtatcactct aggcaaaata gcaccctccc gctccagaac aacatacagc gcttcacagc

     2881 ggcagcctaa cagtcagcct taccagtaaa aaagaaaacc tattaaaaaa acaccactcg

     2941 acacggcacc agctcaatca gtcacagtgt aaaaaagggc caagtgcaga gcgagtatat

     3001 ataggactaa aaaatgacgt aacggttaaa gtccacaaaa aacacccaga aaaccgcacg

     3061 cgaacctacg cccagaaacg aaagccaaaa aacccacaac ttcctcaaat cgtcacttcc

     3121 gttttcccac gttacgtaac ttcccatttt aagaaaacta caattcccaa cacatacaag

     3181 ttactccgcc ctaaaaccta cgtcacccgc cccgttccca cgccccgcgc cacgtcacaa

     3241 actccacccc ctcattatca tattggcttc aatccaaaat aaggtata

//