• 规格：20μl质粒
• 启动子：CMV promoter
• 质粒大小：8141bp
• 原核抗性：氨苄青霉素Amp
• 筛选标记：turboRFP-T2A-Puro
• 克隆菌株：大肠杆菌Stbl3
• 表达宿主：293T等哺乳细胞
• 质粒简介：           pCDH-CMV-MCS-EF1-turboRFP-T2A-Puro是一个慢病毒表达质粒，其配套包装质粒是pLP1，pLP2，pLP/VSVG。本质粒带有红色荧光turboRFP和嘌呤霉素puro标记，可起到很好的筛选作用。
• 质粒图谱1： png/1-1G0220SS4C7.png

质粒序列： 点击查看序列

LOCUS       Exported                8141 bp ds-DNA     circular SYN 22-OCT-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 8141)

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..8141

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     LTR             234..414

                     /label=5' LTR (truncated)

     misc_feature    458..583

                     /label=HIV-1 Psi

     misc_feature    1076..1309

                     /label=RRE

     misc_feature    1804..1920

                     /label=cPPT/CTS

     promoter        2010..2213

                     /label=CMV promoter

     misc_feature    2278..2320

                     /label=MCS

     promoter        2353..2564

                     /label=EF-1-alpha core promoter

     LTR             2577..2845

                     /label=5' LTR (truncated)

     regulatory      2874..2879

     CDS             2880..3575

                     /codon_start=1

                     /label=TurboRFP

                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV

                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA

                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK

                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC

                     DLPSKLGHR"

     CDS             3576..3629

                     /codon_start=1

                     /label=T2A

                     /translation="EGRGSLLTCGDVEENPGP"

     CDS             3630..4229

                     /codon_start=1

                     /label=PuroR

                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER

                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA

                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS

                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"

     misc_feature    4236..4824

                     /label=WPRE

     LTR             4898..5131

                     /label=3' LTR (Delta-U3)

     polyA_signal    5203..5324

                     /label=SV40 poly(A) signal

     rep_origin      5343..5478

                     /label=SV40 ori

     rep_origin      complement(5918..6506)

                     /direction=LEFT

                     /label=ori

     CDS             complement(6677..7537)

                     /codon_start=1

                     /label=AmpR

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(7538..7642)

                     /label=AmpR promoter

ORIGIN

        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca

       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta

      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga

      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc

      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta

      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact

      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg

      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct

      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt

      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag

      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt

      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt

      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg

      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag

      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag

      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg

      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag

     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag

     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc

     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga

     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc

     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg

     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata

     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa

     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga

     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa

     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat

     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt

     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg

     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt

     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat

     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt

     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca

     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc

     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga

     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat

     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag

     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct

     2281 agagctagcg aattcgaatt taaatcggat ccgcggccgc aaggatctgc gatcgctccg

     2341 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg

     2401 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg

     2461 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg

     2521 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg

     2581 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga

     2641 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa

     2701 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac

     2761 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt

     2821 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca

     2881 tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca

     2941 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca

     3001 tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca

     3061 gcttcatgta cggcagcaaa gccttcatca accacaccca gggcatcccc gacttcttta

     3121 agcagtcctt ccctgagggc ttcacatggg agagaatcac cacatacgaa gacgggggcg

     3181 tgctgaccgc tacccaggac accagcttcc agaacggctg catcatctac aacgtcaaga

     3241 tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca cgcggctggg

     3301 aggccaacac cgagatgctg taccccgctg acggcggcct gagaggccac agccagatgg

     3361 ccctgaagct cgtgggcggg ggctacctgc actgctcctt caagaccaca tacagatcca

     3421 agaaacccgc taagaacctc aagatgcccg gcttccactt cgtggaccac agactggaaa

     3481 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgagatggct gtggccaagt

     3541 actgcgacct ccctagcaaa ctggggcaca gatgagaggg cagaggaagt ctgctaacat

     3601 gcggtgacgt cgaggagaat cctggcccaa tgaccgagta caagcccacg gtgcgcctcg

     3661 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc

     3721 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag

     3781 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg

     3841 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga

     3901 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag

     3961 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct

     4021 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg

     4081 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct

     4141 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct

     4201 ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt

     4261 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg

     4321 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt

     4381 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg

     4441 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc

     4501 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg

     4561 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt

     4621 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc

     4681 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg

     4741 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga

     4801 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct

     4861 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa

     4921 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc

     4981 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga

     5041 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga

     5101 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag

     5161 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct

     5221 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca

     5281 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag