质粒序列: 点击查看序列LOCUS Exported 8141 bp ds-DNA circular SYN 22-OCT-2017DEFINITION synthetic circular DNASOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 8141)TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..8141/organism="synthetic DNA construct"/mol_type="other DNA"LTR 234..414/label=5' LTR (truncated)misc_feature 458..583/label=HIV-1 Psimisc_feature 1076..1309/label=RREmisc_feature 1804..1920/label=cPPT/CTSpromoter 2010..2213/label=CMV promotermisc_feature 2278..2320/label=MCSpromoter 2353..2564/label=EF-1-alpha core promoterLTR 2577..2845/label=5' LTR (truncated)regulatory 2874..2879CDS 2880..3575/codon_start=1/label=TurboRFP/translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKVVEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTATQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALKLVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYCDLPSKLGHR"CDS 3576..3629/codon_start=1/label=T2A/translation="EGRGSLLTCGDVEENPGP"CDS 3630..4229/codon_start=1/label=PuroR/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"misc_feature 4236..4824/label=WPRELTR 4898..5131/label=3' LTR (Delta-U3)polyA_signal 5203..5324/label=SV40 poly(A) signalrep_origin 5343..5478/label=SV40 orirep_origin complement(5918..6506)/direction=LEFT/label=oriCDS complement(6677..7537)/codon_start=1/label=AmpR/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(7538..7642)/label=AmpR promoterORIGIN1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct2281 agagctagcg aattcgaatt taaatcggat ccgcggccgc aaggatctgc gatcgctccg2341 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg2401 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg2461 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg2521 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg2581 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga2641 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa2701 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac2761 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt2821 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca2881 tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca2941 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca3001 tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca3061 gcttcatgta cggcagcaaa gccttcatca accacaccca gggcatcccc gacttcttta3121 agcagtcctt ccctgagggc ttcacatggg agagaatcac cacatacgaa gacgggggcg3181 tgctgaccgc tacccaggac accagcttcc agaacggctg catcatctac aacgtcaaga3241 tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca cgcggctggg3301 aggccaacac cgagatgctg taccccgctg acggcggcct gagaggccac agccagatgg3361 ccctgaagct cgtgggcggg ggctacctgc actgctcctt caagaccaca tacagatcca3421 agaaacccgc taagaacctc aagatgcccg gcttccactt cgtggaccac agactggaaa3481 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgagatggct gtggccaagt3541 actgcgacct ccctagcaaa ctggggcaca gatgagaggg cagaggaagt ctgctaacat3601 gcggtgacgt cgaggagaat cctggcccaa tgaccgagta caagcccacg gtgcgcctcg3661 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc3721 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag3781 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg3841 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga3901 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag3961 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct4021 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg4081 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct4141 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct4201 ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt4261 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg4321 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt4381 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg4441 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc4501 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg4561 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt4621 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc4681 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg4741 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga4801 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct4861 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa4921 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc4981 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga5041 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga5101 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag5161 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct5221 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca5281 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag