• 规格：20μl质粒
• 启动子：H1 promoter
• 复制子：pBR322 ori，SV40 ori
• 质粒大小：4555bp
• 原核抗性：氨苄青霉素Amp
• 筛选标记：潮霉素Hyg
• 克隆菌株：大肠杆菌Stbl3
• 表达宿主：哺乳细胞
• 质粒简介：           pSilencer3.1-H1-Stuffer-Hygro是一个哺乳细胞干扰载体，通过BamHI和HindIII酶切位点可连入SiRNA，点击查看本质粒的参考资料：pSilencer hygro Kit.       The pSilencer vectors employ RNA polymerase III (pol III) promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.
• 质粒图谱1： png/1-1G11FZ002147.png

质粒序列： 点击查看序列

LOCUS       Exported                4555 bp ds-DNA     circular SYN 05-NOV-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4555)

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..4555

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        402..492

                     /label=H1 promoter

                     /note="human H1 RNA promoter"

     misc_feature    501..558

                     /label=Stuffer

     rep_origin      complement(979..1567)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(1738..2598)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     polyA_signal    2751..2872

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     CDS             complement(3002..4027)

                     /codon_start=1

                     /gene="aph(4)-Ia"

                     /product="aminoglycoside phosphotransferase from E. coli"

                     /label=HygR

                     /note="confers resistance to hygromycin"

                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG

                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP

                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ

                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF

                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG

                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK

                     E"

     promoter        complement(4084..4294)

                     /label=SV40 promoter

                     /note="SV40 early promoter"

     rep_origin      4090..4225

                     /label=SV40 ori

                     /note="SV40 origin of replication"

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt catatttgca tgtcgctatg

      421 tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct tataagttct

      481 gtatgagacc actcggatcc cagctcacaa ccaggatact ttcaagagaa gtatcctggt

      541 tgtgagcttt ttttggaaaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa

      601 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg

      661 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca

      721 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg

      781 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg

      841 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg

      901 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa

      961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg

     1021 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc

     1081 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc

     1141 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc

     1201 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg

     1261 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc

     1321 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga

     1381 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc

     1441 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac

     1501 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg

     1561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc

     1621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa

     1681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta

     1741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt

     1801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag

     1861 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca

     1921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc

     1981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt

     2041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag

     2101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt

     2161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat

     2221 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt

     2281 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc

     2341 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat

     2401 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag

     2461 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt

     2521 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg

     2581 gaaatgttga atactcatac tcttcctttt tcaatattca ggctttacac tttatgcttc

     2641 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg

     2701 accatgatta cgccaagctc tagctagagg tcgacggtat acagacatga taagatacat

     2761 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat

     2821 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttggggtggg

     2881 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa

     2941 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtagcacgt

     3001 gctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt

     3061 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag

     3121 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga

     3181 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc

     3241 ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct

     3301 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc

     3361 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat

     3421 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa

     3481 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt

     3541 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt

     3601 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag

     3661 cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca

     3721 ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga

     3781 attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa

     3841 cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc

     3901 ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg

     3961 agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct

     4021 ttttcatcac gtgctgatca gatccgaaaa tggatataca agctcccggg agctttttgc

     4081 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggcagagg

     4141 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg

     4201 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact

     4261 aattgagatg catgcttcgc atacttctgc ctgctgggga gcctggggac tttccacacc

     4321 aggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctgga atattattga

     4381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat

     4441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc

     4501 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc

//