- 规格:20μl质粒
- 原核抗性:卡那霉素Kan
- 筛选标记:潮霉素Hyg
- 克隆菌株:大肠杆菌DB3.1
- 质粒简介: pCXUN-FLAG是一个植物pCX系列蛋白表达质粒。
- 质粒图谱1: png/1-1P110142622159.png
质粒序列: 点击查看序列LOCUS Exported 12035 bp ds-DNA circular SYN 04-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..12035/organism="synthetic DNA construct"/mol_type="other DNA"terminator 115..367/label=NOS terminator/note="nopaline synthase terminator and poly(A) signal"CDS 728..1033/codon_start=1/gene="ccdB"/product="CcdB, a bacterial toxin that poisons DNA gyrase"/label=ccdB/note="Plasmids containing the ccdB gene cannot bepropagated in standard E. coli strains."/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"CDS complement(1089..1112)/codon_start=1/product="FLAG(R) epitope tag, followed by an enterokinasecleavage site"/label=FLAG/translation="DYKDDDDK"promoter complement(1128..3118)/label=Ubi promoter/note="maize polyubiquitin gene promoter"misc_feature 3359..3383/label=RB T-DNA repeat/note="right border repeat from nopaline C58 T-DNA"CDS 4682..5311/codon_start=1/product="stability protein from the Pseudomonas plasmidpVS1 (Heeb et al., 2000)"/label=pVS1 StaA/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"CDS 5745..6812/codon_start=1/product="replication protein from the Pseudomonas plasmidpVS1 (Heeb et al., 2000)"/label=pVS1 RepA/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"rep_origin 6878..7072/label=pVS1 oriV/note="origin of replication for the Pseudomonas plasmidpVS1 (Heeb et al., 2000)"misc_feature 7416..7556/label=bom/note="basis of mobility region from pBR322"rep_origin complement(7742..8330)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(8417..9211)/codon_start=1/gene="aphA-3"/product="aminoglycoside phosphotransferase"/label=KanR/note="confers resistance to kanamycin"/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"misc_feature 9636..9660/label=LB T-DNA repeat/note="left border repeat from nopaline C58 T-DNA"polyA_signal 9738..9912/label=CaMV poly(A) signal/note="cauliflower mosaic virus polyadenylation signal"CDS complement(9952..10977)/codon_start=1/gene="aph(4)-Ia"/product="aminoglycoside phosphotransferase from E. coli"/label=HygR/note="confers resistance to hygromycin"/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKK"promoter complement(11044..11721)/label=CaMV 35S promoter (enhanced)/note="cauliflower mosaic virus 35S promoter with aduplicated enhancer region"ORIGIN1 gaattcgagc tcggtacccc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt61 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccgatcta121 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt181 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata241 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga301 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg361 aacgatcggg gaaattcgga tccccaatac ttgtatgggg cttactaaaa gccagataac421 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc481 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga541 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc601 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg661 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc721 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt781 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag841 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga901 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga961 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt1021 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gacccataca1081 agtattggct tatcatcatc atccttgtaa tccatggatc ccccgggctg cagaagtaac1141 accaaacaac agggtgagca tcgacaaaag aaacagtacc aagcaaataa atagcgtatg1201 aaggcagggc taaaaaaatc cacatatagc tgctgcatat gccatcatcc aagtatatca1261 agatcaaaat aattataaaa catacttgtt tattataata gataggtact caaggttaga1321 gcatatgaat agatgctgca tatgccatca tgtatatgca tcagtaaaac ccacatcaac1381 atgtatacct atcctagatc gatatttcca tccatcttaa actcgtaact atgaagatgt1441 atgacacaca catacagttc caaaattaat aaatacacca ggtagtttga aacagtattc1501 tactccgatc tagaacgaat gaacgaccgc ccaaccacac cacatcatca caaccaagcg1561 aacaaaaagc atctctgtat atgcatcagt aaaacccgca tcaacatgta tacctatcct1621 agatcgatat ttccatccat catcttcaat tcgtaactat gaatatgtat ggcacacaca1681 tacagatcca aaattaataa atccaccagg tagtttgaaa cagaattcta ctccgatcta1741 gaacgaccgc ccaaccagac cacatcatca caaccaagac aaaaaaaagc atgaaaagat1801 gacccgacaa acaagtgcac ggcatatatt gaaataaagg aaaagggcaa accaaaccct1861 atgcaacgaa acaaaaaaaa tcatgaaatc gatcccgtct gcggaacggc tagagccatc1921 ccaggattcc ccaaagagaa acactggcaa gttagcaatc agaacgtgtc tgacgtacag1981 gtcgcatccg tgtacgaacg ctagcagcac ggatctaaca caaacacgga tctaacacaa2041 acatgaacag aagtagaact accgggccct aaccatggac cggaacgccg atctagagaa2101 ggtagagagg gggggggggg gaggacgagc ggcgtacctt gaagcggagg tgccgacggg2161 tggatttggg ggagatctgg ttgtgtgtgt gtgcgctccg aacaacacga ggttggggaa2221 agagggtgtg gagggggtgt ctatttatta cggcgggcga ggaagggaaa gcgaaggagc2281 ggtgggaaag gaatcccccg tagctgccgt gccgtgagag gaggaggagg ccgcctgccg2341 tgccggctca cgtctgccgc tccgccacgc atttctggat gccgacagcg gagcaagtcc2401 aacggtggag cggaactctc gagaggggtc cagaggcagc gacagagatg ccgtgccgtc2461 tgcttcgctt ggcccgacgc gacgctgctg gttcgctggt tggtgtccgt tagactcgtc2521 gacggcgttt aacaggctgg cattatctac tcgaaacaag aaaaatgttt ccttagtttt2581 tttaatttct taaagggtat ttgtttaatt tttagtcact ttattttatt ctattttata2641 tctaaattat taaataaaaa aactaaaata gagttttagt tttcttaatt tagaggctaa2701 aatagaataa aatagatgta ctaaaaaaat tagtctataa aaaccattaa ccctaaaccc2761 taaatggatg tactaataaa atggatgaag tattatatag gtgaagctat ttgcaaaaaa2821 aaaggagaac acatgcacac taaaaagata aaactgtaga gtcctgttgt caaaatactc2881 aattgtcctt tagaccatgt ctaactgttc atttatatga ttctctaaaa cactgatatt2941 attgtagtac tatagattat attattcgta gagtaaagtt taaatatatg tataaagata3001 gataaactgc acttcaaaca agtgtgacaa aaaaaatatg tggtaatttt ttataactta3061 gacatgcaat gctcattatc tctagagagg ggcacgaccg ggtcacgctg cactgcagga3121 attcgatatc aagcttggca ctggccgtcg ttttacaacg t