• 规格：20μl质粒
• 原核抗性：庆大霉素Gen
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：            pMP2444是一个广宿主原核绿色荧光蛋白表达质粒。
• 质粒图谱1： png/1-1G205095T0450.png

质粒序列： 点击查看序列

LOCUS       Exported                5471 bp ds-DNA     circular SYN 04-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5471

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             complement(261..794)

                     /codon_start=1

                     /gene="aacC1"

                     /product="gentamycin acetyltransferase"

                     /label=Gen

                     /note="confers resistance to gentamycin"

                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD

                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS

                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR

                     EEVMHFDIDPSTAT"

     promoter        complement(983..1011)

                     /gene="intI1 (promoter lies within the coding sequence)"

                     /label=Pc promoter

                     /note="class 1 integron promoter"

     protein_bind    1281..1302

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence 

                     of cAMP."

     promoter        1317..1347

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    1355..1371

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        1416..1434

                     /label=T3 promoter

                     /note="promoter for bacteriophage T3 RNA polymerase"

     misc_feature    1447..1518

                     /label=MCS

     CDS             1520..2239

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     CDS             complement(3017..3679)

                     /codon_start=1

                     /product="replication protein for the broad-host-range 

                     plasmid pBBR1 from Bordetella bronchiseptica"

                     /label=pBBR1 Rep

                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH

                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV

                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE

                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"

     rep_origin      3680..4449

                     /label=pBBR1 oriV

                     /note="replication origin of the broad-host-range plasmid 

                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 

                     Rep protein for replication"

ORIGIN

        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc

       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg

      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg

      181 aaaagtgcca cctggcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg

      241 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact

      301 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct

      361 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc

      421 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc

      481 tcactacgcg gctgctcaaa cctgggcaga acgtaagccg cgagagcgcc aacaaccgct

      541 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa

      601 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga

      661 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg

      721 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg

      781 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc

      841 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa

      901 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga

      961 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg

     1021 tgccttcatc cgtttccacg gtgtgcgtcc atgggcaaat attatacgca aggcgacaag

     1081 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc

     1141 agaatgctta atgaattaca acagttttta tgcatgcgcc caatacgcaa accgcctctc

     1201 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg

     1261 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta

     1321 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca

     1381 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa

     1441 aagctgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc

     1501 ctgcagcccg ggggatccaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc

     1561 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg

     1621 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct

     1681 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg

     1741 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt

     1801 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa

     1861 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga

     1921 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat

     1981 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga

     2041 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt

     2101 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga

     2161 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat

     2221 ggacgagctg tacaagtagt ctagattagt ggagctccaa ttcgccctat agtgagtcgt

     2281 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta

     2341 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg

     2401 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag

     2461 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca

     2521 ataggccgac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg

     2581 cccttaaacg cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat

     2641 tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg

     2701 tctattgctg gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa

     2761 atgcctgagg ccagtttgct caggctctcc ccgtggaggt aataattgac gatatgatca

     2821 tttattctgc ctcccagagc ctgataaaaa cggtgaatcc gttagcgagg tgccgccggc

     2881 ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac

     2941 aaggtatagg gcggcgaggc ggctacagcc gatagtctgg aacagcgcac ttacgggttg

     3001 ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg tgtcggcggc tccaacggct

     3061 cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag gcgctgctgc ccgcgccgtt

     3121 cccattcctc cgtttcggtc aaggctggca ggtctggttc catgcccgga atgccgggct

     3181 ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg ctcgcccgga tacagggtcg

     3241 ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc ctggtcgtcg tgatcaacca

     3301 ccacggcggc actgaacacc gacaggcgca actggtcgcg gggctggccc cacgccacgc

     3361 ggtcattgac cacgtaggcc gacacggtgc cggggccgtt gagcttcacg acggagatcc

     3421 agcgctcggc caccaagtcc ttgactgcgt attggaccgt ccgcaaagaa cgtccgatga

     3481 gcttggaaag tgtcttctgg ctgaccacca cggcgttctg gtggcccatc tgcgccacga

     3541 ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat aagcccggcc cacgcctcat

     3601 gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt cttggcttga atgccgattt

     3661 ctctggactg cgtggccatg cttatctcca tgcggtaggg tgccgcacgg ttgcggcacc

     3721 atgcgcaatc agctgcaact tttcggcagc gcgacaacaa ttatgcgttg cgtaaaagtg

     3781 gcagtcaatt acagattttc tttaacctac gcaatgagct attgcggggg gtgccgcaat

     3841 gagctgttgc gtacccccct tttttaagtt gttgattttt aagtctttcg catttcgccc

     3901 tatatctagt tctttggtgc ccaaagaagg gcacccctgc ggggttcccc cacgccttcg

     3961 gcgcggctcc ccctccggca aaaagtggcc cctccggggc ttgttgatcg actgcgcggc

     4021 cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc ccgcactcgc cgccgtgagg

     4081 ctcggggggc aggcgggcgg gcttcgcctt cgactgcccc cactcgcata ggcttgggtc

     4141 gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt

     4201 ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc aggccggagg cttttcccca

     4261 gagaaaatta aaaaaattga tggggcaagg ccgcaggccg cgcagttgga gccggtgggt

     4321 atgtggtcga aggctgggta gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg

     4381 cagcctgtcc atcagcttgt ccagcagggt tgtccacggg ccgagcgaag cgagccagcc

     4441 ggtggccgct cgcggccatc gtccacatat ccacgggctg gcaagggagc gcagcgaccg

     4501 cgcagggcga agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg

     4561 actttgcgaa gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc

     4621 gccttgcgct gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc

     4681 atacgcgatc atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa

     4741 gcacgcctac cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga

     4801 gcactgggcg gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga

     4861 gaagcggcgc aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg

     4921 gtggaagtcg gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct

     4981 ggcggacaag tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag

     5041 cccgcacatg accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga

     5101 gttcatcggc aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc

     5161 cgatctaggg ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc

     5221 gttctacgag gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt

     5281 cgagccacgc gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt