• 规格：20μl质粒
• 原核抗性：卡那霉素Kan
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：            pLTR-G是一个慢病毒哺乳细胞VSVG包膜蛋白表达质粒。
• 质粒图谱1： jpg/1-1G2040U323137.jpg

质粒序列： 点击查看序列

LOCUS       Exported                5832 bp ds-DNA     circular SYN 03-NOV-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5832

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             782..2317

                     /codon_start=1

                     /product="vesicular stomatitis virus G glycoprotein"

                     /label=VSV-G

                     /note="Indiana strain"

                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD

                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS

                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ

                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR

                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT

                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK

                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP

                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF

                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"

     polyA_signal    2620..2741

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(2748..3203)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        3230..3334

                     /gene="bla"

                     /label=AmpR promoter

     promoter        3336..3693

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      3544..3679

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             3728..4522

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    4754..4801

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      5130..5718

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 tagttattac cggactcaga tctcgagacc tagaaaaaca tggagcaatc acaagtagca

       61 atacagcagc taacaatgct gcttgtgcct ggctagaagc acaagaggag gaagaggtgg

      121 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc

      181 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagac

      241 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact

      301 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac

      361 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc

      421 ctgtgagcct gcatggaatg gatgaccctg agagagaagt gttagagtgg aggtttgaca

      481 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct

      541 gacatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg

      601 gcgggactgg ggagtggcga gccctcagat gctgcatata agcagctgct ttttgcctgt

      661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac

      721 ccactgctta agcctcaata aagcttctag agatccctcg acctcgagga attctgacac

      781 tatgaagtgc cttttgtact tagccttttt attcattggg gtgaattgca agttcaccat

      841 agtttttcca cacaaccaaa aaggaaactg gaaaaatgtt ccttctaatt accattattg

      901 cccgtcaagc tcagatttaa attggcataa tgacttaata ggcacagcct tacaagtcaa

      961 aatgcccaag agtcacaagg ctattcaagc agacggttgg atgtgtcatg cttccaaatg

     1021 ggtcactact tgtgatttcc gctggtatgg accgaagtat ataacacatt ccatccgatc

     1081 cttcactcca tctgtagaac aatgcaagga aagcattgaa caaacgaaac aaggaacttg

     1141 gctgaatcca ggcttccctc ctcaaagttg tggatatgca actgtgacgg atgccgaagc

     1201 agtgattgtc caggtgactc ctcaccatgt gctggttgat gaatacacag gagaatgggt

     1261 tgattcacag ttcatcaacg gaaaatgcag caattacata tgccccactg tccataactc

     1321 tacaacctgg cattctgact ataaggtcaa agggctatgt gattctaacc tcatttccat

     1381 ggacatcacc ttcttctcag aggacggaga gctatcatcc ctgggaaagg agggcacagg

     1441 gttcagaagt aactactttg cttatgaaac tggaggcaag gcctgcaaaa tgcaatactg

     1501 caagcattgg ggagtcagac tcccatcagg tgtctggttc gagatggctg ataaggatct

     1561 ctttgctgca gccagattcc ctgaatgccc agaagggtca agtatctctg ctccatctca

     1621 gacctcagtg gatgtaagtc taattcagga cgttgagagg atcttggatt attccctctg

     1681 ccaagaaacc tggagcaaaa tcagagcggg tcttccaatc tctccagtgg atctcagcta

     1741 tcttgctcct aaaaacccag gaaccggtcc tgctttcacc ataatcaatg gtaccctaaa

     1801 atactttgag accagataca tcagagtcga tattgctgct ccaatcctct caagaatggt

     1861 cggaatgatc agtggaacta ccacagaaag ggaactgtgg gatgactggg caccatatga

     1921 agacgtggaa attggaccca atggagttct gaggaccagt tcaggatata agtttccttt

     1981 atacatgatt ggacatggta tgttggactc cgatcttcat cttagctcaa aggctcaggt

     2041 gttcgaacat cctcacattc aagacgctgc ttcgcaactt cctgatgatg agagtttatt

     2101 ttttggtgat actgggctat ccaaaaatcc aatcgagctt gtagaaggtt ggttcagtag

     2161 ttggaaaagc tctattgcct cttttttctt tatcataggg ttaatcattg gactattctt

     2221 ggttctccga gttggtatcc atctttgcat taaattaaag cacaccaaga aaagacagat

     2281 ttatacagac atagagatga accgacttgg aaagtaactc aaatcctgca caacagattc

     2341 ttcatgtttg gaccaaatca acttgtgata ccatgctcaa agaggcctca attatatttg

     2401 agtttttaat ttttatgaaa aaaaaaaaaa aaaacggaat tcctcgaggg atcttccata

     2461 cctaccagtt ctgcgcctgc aggtcgcggc cgatccaccg gatctagata actgatcata

     2521 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc

     2581 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat

     2641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg

     2701 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt

     2761 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata

     2821 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt

     2881 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg

     2941 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt

     3001 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc

     3061 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg

     3121 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct

     3181 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc

     3241 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg

     3301 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg

     3361 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa

     3421 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc

     3481 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg

     3541 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt

     3601 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga

     3661 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc

     3721 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag

     3781 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg

     3841 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa

     3901 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc

     3961 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc

     4021 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga

     4081 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa

     4141 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct

     4201 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat

     4261 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt

     4321 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta

     4381 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga

     4441 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg

     4501 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg

     4561 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc

     4621 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag

     4681 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga

     4741 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt

     4801 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat

     4861 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa

     4921 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca

     4981 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc

     5041 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca

     5101 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc

     5161 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta

     5221 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt

     5281 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc

     5341 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg

     5401 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg

     5461 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag

     5521 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc

     5581 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat

     5641 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg

     5701 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc