• 规格：20μl质粒
• 原核抗性：卡那霉素Kan
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：            DsRed-rab7 WT人源基因哺乳细胞过表达质粒。
• 质粒图谱1： png/1-1G203113021F1.png

质粒序列： 点击查看序列

LOCUS       Exported                6060 bp ds-DNA     circular SYN 03-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..6060

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        68..371

                     /note="CMV enhancer"

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        372..575

                     /note="CMV promoter"

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     regulatory      614..623

                     /regulatory_class="other"

                     /note="Kozak sequence"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     CDS             620..1297

                     /codon_start=1

                     /product="wild-type DsRed"

                     /note="DsRed1"

                     /note="mammalian codon-optimized"

                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV

                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG

                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH

                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH

                     HLFL"

     CDS             1361..1387

                     /codon_start=1

                     /product="HA (human influenza hemagglutinin) epitope tag"

                     /note="HA"

                     /translation="YPYDVPDYA"

     CDS             1388..1414

                     /codon_start=1

                     /product="HA (human influenza hemagglutinin) epitope tag"

                     /note="HA"

                     /translation="YPYDVPDYA"

     misc_feature    1415..2038

                     /note="RAB7"

     polyA_signal    2855..2976

                     /note="SV40 poly(A) signal"

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(2983..3438)

                     /direction=LEFT

                     /note="f1 ori"

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        3465..3569

                     /gene="bla"

                     /note="AmpR promoter"

     promoter        3571..3928

                     /note="SV40 promoter"

                     /note="SV40 enhancer and early promoter"

     rep_origin      3779..3914

                     /note="SV40 ori"

                     /note="SV40 origin of replication"

     CDS             3963..4757

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /note="NeoR/KanR"

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    4989..5036

                     /note="HSV TK poly(A) signal"

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      5365..5953

                     /direction=RIGHT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg

       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc

      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt

      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc

      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg

      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg

      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact

      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa

      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta

      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct

      601 agcgctaccg gtcgccacca tggtgcgctc ctccaagaac gtcatcaagg agttcatgcg

      661 cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg

      721 cgagggccgc ccctacgagg gccacaacac cgtgaagctg aaggtgacca agggcggccc

      781 cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt

      841 gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg

      901 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct

      961 gcaggacggc tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg

     1021 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg

     1081 cgacggcgtg ctgaagggcg agatccacaa ggccctgaag ctgaaggacg gcggccacta

     1141 cctggtggag ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta

     1201 ctacgtggac tccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca

     1261 gtacgagcgc accgagggcc gccaccacct gttcctgctc agatctcgag ctcaagcttc

     1321 gaattctgca gtcgacggta ccgcgggccc gggatccatg tacccatacg atgttccgga

     1381 ttacgcttac ccatacgatg ttccggatta cgctggtacc tctaggaaga aagtgttgct

     1441 gaaggttatc atcctgggag attctggagt cgggaagaca tcactcatga accagtatgt

     1501 gaataagaaa ttcagcaatc agtacaaagc cacaatagga gctgactttc tgaccaagga

     1561 ggtgatggtg gatgacaggc tagtcacaat gcagatatgg gacacagcag gacaggaacg

     1621 gttccagtct ctcggtgtgg ccttctacag aggtgcagac tgctgcgttc tggtatttga

     1681 tgtgactgcc cccaacacat tcaaaaccct agatagctgg agagatgagt ttctcatcca

     1741 ggccagtccc cgagatcctg aaaacttccc atttgttgtg ttgggaaaca agattgacct

     1801 cgaaaacaga caagtggcca caaagcgggc acaggcctgg tgctacagca aaaacaacat

     1861 tccctacttt gagaccagtg ccaaggaggc catcaacgtg gagcaggcgt tccagacgat

     1921 tgcacggaat gcacttaagc aggaaacgga ggtggagctg tacaacgaat ttcctgaacc

     1981 tatcaaactg gacaagaatg accgggccaa ggcctcggca gaaagctgca gttgctgagg

     2041 atccatgtac ccatacgatg ttccggatta cgcttaccca tacgatgttc cggattacgc

     2101 tggtacctct aggaagaaag tgttgctgaa ggttatcatc ctgggagatt ctggagtcgg

     2161 gaagacatca ctcatgaacc agtatgtgaa taagaaattc agcaatcagt acaaagccac

     2221 aataggagct gactttctga ccaaggaggt gatggtggat gacaggctag tcacaatgca

     2281 gatatgggac acagcaggac aggaacggtt ccagtctctc ggtgtggcct tctacagagg

     2341 tgcagactgc tgcgttctgg tatttgatgt gactgccccc aacacattca aaaccctaga

     2401 tagctggaga gatgagtttc tcatccaggc cagtccccga gatcctgaaa acttcccatt

     2461 tgttgtgttg ggaaacaaga ttgacctcga aaacagacaa gtggccacaa agcgggcaca

     2521 ggcctggtgc tacagcaaaa acaacattcc ctactttgag accagtgcca aggaggccat

     2581 caacgtggag caggcgttcc agacgattgc acggaatgca cttaagcagg aaacggaggt

     2641 ggagctgtac aacgaatttc ctgaacctat caaactggac aagaatgacc gggccaaggc

     2701 ctcggcagaa agctgcagtt gctgaggatc caccggatct agataactga tcataatcag

     2761 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa

     2821 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg

     2881 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc

     2941 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata

     3001 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg

     3061 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc

     3121 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa

     3181 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt

     3241 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac

     3301 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta

     3361 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg

     3421 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt

     3481 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa

     3541 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt

     3601 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat

     3661 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag

     3721 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat

     3781 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt

     3841 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg

     3901 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc

     3961 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat

     4021 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt

     4081 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac

     4141 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg

     4201 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc

     4261 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa

     4321 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc

     4381 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg

     4441 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg