- 商品货号:FH1674
- 规格:10μl质粒
- 原核抗性:氨苄青霉素Amp
- 克隆菌株:大肠杆菌Stbl3
- 质粒简介: BE4-Gam是一个大鼠基因哺乳细胞过表达质粒。
- 质粒图谱1: png/1-1G201162120P9.png
质粒序列: 点击查看序列LOCUS Exported 9444 bp ds-DNA circular SYN 01-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..9444/organism="synthetic DNA construct"/mol_type="other DNA"promoter 132..335/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 377..395/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"regulatory 403..412/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"misc_feature 409..930/label=GamCDS 979..1662/codon_start=1/product="cytidine deaminase (C to U editing enzyme) fromrat"/label=rAPOBEC-1/note="can use ssDNA as a substrate (Komor et al., 2016)"/translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHILWATGLK"CDS 1759..5859/codon_start=1/product="nickase mutant of the Cas9 endonuclease from theStreptococcus pyogenes Type II CRISPR/Cas system"/label=Cas9(D10A)/note="generates RNA-guided single strand nicks in DNA dueto the D10A mutation in the RuvC catalytic domain"/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"CDS 5890..6138/codon_start=1/product="uracil-DNA glycosylase inhibitor from a Bacillussubtilis bacteriophage (Mol et al., 1995)"/label=UGI/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"CDS 6169..6417/codon_start=1/product="uracil-DNA glycosylase inhibitor from a Bacillussubtilis bacteriophage (Mol et al., 1995)"/label=UGI/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"CDS 6430..6450/codon_start=1/product="nuclear localization signal of SV40 (simian virus40) large T antigen"/label=SV40 NLS/translation="PKKKRKV"CDS 6459..6476/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"polyA_signal 6505..6729/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin complement(7202..7790)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(7961..8821)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(8822..8926)/gene="bla"/label=AmpR promoterenhancer 9196..131/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"ORIGIN1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggctaaacca421 gcaaaacgta tcaagagtgc cgcagcggct tatgtgccac aaaaccgcga tgcggtgatt481 accgatatta aacgcatcgg ggatttacag cgcgaagcat cacgtctgga aacggaaatg541 aatgatgcca tcgcggaaat tacggagaaa tttgcggccc ggattgcacc gattaaaacc601 gatattgaaa ccctttcaaa aggcgttcag ggatggtgtg aagcgaaccg cgacgaactg661 acgaacggcg gcaaagtgaa gacggcgaat cttgtcaccg gtgatgtatc gtggcgggtc721 cgtccaccat cagtaagtat tcgtggtatg gatgcagtga tggaaacgct ggagcgtctt781 ggcctgcaac gctttattcg cacgaagcag gaaatcaaca aggaagcgat tttactggaa841 ccgaaagcgg tcgcaggcgt tgccggaatt acagttaaat caggcattga ggatttttct901 attattccat ttgaacagga agccggtatt agcggcagcg agactcccgg gacctcagag961 tccgctacac ccgaaagtag ctcagagact ggcccagtgg ctgtggaccc cacattgaga1021 cggcggatcg agccccatga gtttgaggta ttcttcgatc cgagagagct ccgcaaggag1081 acctgcctgc tttacgaaat taattggggg ggccggcact ccatttggcg acatacatca1141 cagaacacta acaagcacgt cgaagtcaac ttcatcgaga agttcacgac agaaagatat1201 ttctgtccga acacaaggtg cagcattacc tggtttctca gctggagccc atgcggcgaa1261 tgtagtaggg ccatcactga attcctgtca aggtatcccc acgtcactct gtttatttac1321 atcgcaaggc tgtaccacca cgctgacccc cgcaatcgac aaggcctgcg ggatttgatc1381 tcttcaggtg tgactatcca aattatgact gagcaggagt caggatactg ctggagaaac1441 tttgtgaatt atagcccgag taatgaagcc cactggccta ggtatcccca tctgtgggta1501 cgactgtacg ttcttgaact gtactgcatc atactgggcc tgcctccttg tctcaacatt1561 ctgagaagga agcagccaca gctgacattc tttaccatcg ctcttcagtc ttgtcattac1621 cagcgactgc ccccacacat tctctgggcc accgggttga aatctggtgg ttcttctggt1681 ggttctagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga aagttctggt1741 ggttcttctg gtggttctga taaaaagtat tctattggtt tagccatcgg cactaattcc1801 gttggatggg ctgtcataac cgatgaatac aaagtacctt caaagaaatt taaggtgttg1861 gggaacacag accgtcattc gattaaaaag aatcttatcg gtgccctcct attcgatagt1921 ggcgaaacgg cagaggcgac tcgcctgaaa cgaaccgctc ggagaaggta tacacgtcgc1981 aagaaccgaa tatgttactt acaagaaatt tttagcaatg agatggccaa agttgacgat
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