• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：        pMT/V5-His is a 3.5 kb expression vector designed for use with the Drosophila Inducible Expression System. Upon transfection, the vector allows transient, inducible expression of your protein of interest in Drosophila cells. When cotransfected with the selection vectors, pCoHygro or pCoBlast, included with the appropriate DES® Inducible Kit, pMT/V5-His allows selection of stable cell lines exhibiting inducible expression of the protein of interest.
• 质粒图谱1： png/1-1G12611351G02.png

质粒序列： 点击查看序列

LOCUS       Exported                3538 bp ds-DNA     circular SYN 26-NOV-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 3538)

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..3538

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        407..833

                     /label=MT promoter

                     /note="Drosophila metallothionein promoter"

     CDS             930..971

                     /codon_start=1

                     /product="epitope tag from simian virus 5"

                     /label=V5 tag

                     /translation="GKPIPNPLLGLDST"

     CDS             981..998

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /label=6xHis

                     /translation="HHHHHH"

     polyA_signal    1040..1174

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(1720..2308)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(2479..3339)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(3340..3444)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattaatt cgttgcagga

      421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc

      481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt

      541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagcttt gcatcccata

      601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac

      661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag

      721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc

      781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc

      841 tagatcgggg tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc

      901 ggccgctcga gtctagaggg cccttcgaag gtaagcctat ccctaaccct ctcctcggtc

      961 tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag

     1021 cctcgactgt gccttctaag atccagacat gataagatac attgatgagt ttggacaaac

     1081 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt

     1141 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat

     1201 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg

     1261 tggtatggct gattatgatc agtcgacctg caggcatgca agcttggcgt aatcatggtc

     1321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg

     1381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt

     1441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg

     1501 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga

     1561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat

     1621 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca

     1681 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc

     1741 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata

     1801 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc

     1861 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc

     1921 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga

     1981 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc

     2041 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag

     2101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag

     2161 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag

     2221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca

     2281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga

     2341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat

     2401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga

     2461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg

     2521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga

     2581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc

     2641 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac

     2701 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc

     2761 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc

     2821 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc

     2881 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt

     2941 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc

     3001 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg

     3061 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag

     3121 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat

     3181 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc

     3241 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa

     3301 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta

     3361 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa

     3421 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga

     3481 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgt

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