• 规格：20μl质粒
• 启动子：Drosophila copia
• 质粒分类：昆虫细胞表达载体
• 质粒大小：4526 bp
• 原核抗性：Amp
• 筛选标记：hygromycin
• 克隆菌株：TOP10；DH5-T1R
• 表达宿主：果蝇细胞系S2

质粒序列： 点击查看序列

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg 421 actctagagg atccggtgcg tggtggtttc atgcttctgg gaacggcaaa tgggtttagg 481 attgggaacc cctcatcatc tgttggaata tactattcaa cctacaaaaa taacgttaaa 541 caacactact ttatatttga tatgaatggc cacacctttt atgccataaa acatattgta 601 agagaatacc actcttttta ttccttcttt ccttcttgta cgttttttgc tgtgagtagg 661 tcgtggtgct ggtgttgcag ttgaaataac ttaaaatata aatcataaaa ctcaaacata 721 aacttgacta tttatttatt tattaagaaa ggaaatataa attataaatt acaacaggtt 781 atggggatcc ccagcttggc caccatggga aagcctgagc gtaccgcgac gtctgtcgag 841 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa 901 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc 961 tgcggcgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc 1021 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc 1081 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg 1141 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg 1201 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc 1261 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg 1321 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg 1381 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca 1441 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc 1501 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg 1561 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac 1621 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga 1681 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga 1741 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc 1801 cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa gagctgattt 1861 cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga gagaacggcc 1921 ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg 1981 gagggccggt cgcagtgatt caggcccttc tggattgtgt tggtccccag ggcacgattg 2041 tcatgcccac gcactcgggt gatctgactg atcccgcaga ttggagatcg ccgcccgtgc 2101 ctgccgattg ggtgcagatc agcctcgagg ccagctagct tgaacttgtt tattgcagct 2161 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 2221 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcggg 2281 taccgagctc gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct 2341 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 2401 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 2461 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 2521 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 2581 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 2641 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 2701 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 2761 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 2821 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 2881 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 2941 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 3001 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 3061 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 3121 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 3181 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 3241 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 3301 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 3361 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 3421 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 3481 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 3541 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 3601 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 3661 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 3721 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 3781 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 3841 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 3901 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 3961 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 4021 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 4081 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 4141 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 4201 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 4261 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 4321 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 4381 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 4441 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 4501 taggcgtatc acgaggccct ttcgtc