• 规格：20μl质粒
• 原核抗性：卡那霉素Kan
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：           DsRed-Rab5 WT质粒
• 质粒图谱1： png/1-1G126112G1K1.png

质粒序列： 点击查看序列

LOCUS       Exported                5369 bp ds-DNA     circular SYN 26-NOV-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5369)

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..5369

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        16..319

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        320..523

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     CDS             568..1242

                     /codon_start=1

                     /product="improved tetrameric variant of DsRed fluorescent 

                     protein"

                     /label=DsRed2

                     /note="mammalian codon-optimized"

                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK

                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV

                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK

                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH

                     LFL"

     CDS             1261..1287

                     /codon_start=1

                     /product="HA (human influenza hemagglutinin) epitope tag"

                     /label=HA

                     /translation="YPYDVPDYA"

     misc_feature    1291..1935

                     /label=RAB5A

                     /note="NM_001003317.2"

     polyA_signal    2112..2233

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(2240..2695)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2722..2826

                     /gene="bla"

                     /label=AmpR promoter

     promoter        2828..3185

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      3036..3171

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             3220..4014

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    4246..4293

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      4622..5210

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa

       61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac

      121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca

      181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg

      241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt

      301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg

      361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg

      421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat

      481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca

      541 gatccgctag cgctaccggt cgccaccatg gcctcctccg agaacgtcat caccgagttc

      601 atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc acgagttcga gatcgagggc

      661 gagggcgagg gccgccccta cgagggccac aacaccgtga agctgaaggt gaccaagggc

      721 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg

      781 tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc

      841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg cgaccgtgac ccaggactcc

      901 tccctgcagg acggctgctt catctacaag gtgaagttca tcggcgtgaa cttcccctcc

      961 gacggccccg tgatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac

     1021 ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc tgaagctgaa ggacggcggc

     1081 cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca gctgcccggc

     1141 tactactacg tggacgccaa gctggacatc acctcccaca acgaggacta caccatcgtg

     1201 gagcagtacg agcgcaccga gggccgccac cacctgttcc tgagatctgc cgccatggca

     1261 tatccatatg acgtcccaga ctatgctggc gctagtcgag gcgcaacaag acccaacggg

     1321 ccaaatactg gaaataaaat atgccagttc aaactagtac ttctgggaga gtctgctgtt

     1381 ggcaaatcaa gcctagtgct tcgttttgtg aagggccaat ttcatgaatt tcaagagagt

     1441 accatagggg ctgcttttct aacccaaact gtgtgtcttg atgatacaac agtaaagttt

     1501 gaaatatggg atacagctgg tcaagaacga taccatagct tagcaccaat gtactacaga

     1561 ggagcacaag cagccatagt tgtatatgat atcacaaatg aggagtcctt tgccagagcc

     1621 aaaaactggg ttaaagaact tcagaggcaa gccagtccta acattgtaat agctttatca

     1681 ggaaacaagg ctgatcttgc aaataaaaga gctgtcgatt tccaggaagc acagtcctat

     1741 gcagatgaca acagtttatt attcatggag acatcagcta aaacatcgat gaatgtaaat

     1801 gaaatattca tggcaatagc taaaaagttg ccaaagaacg aaccacagaa tccaggagca

     1861 aattctgcca gaggaagagg agtagacctt actgaaccca cgcagccaac caggaatcag

     1921 tgttgtagta actagctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc

     1981 cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt

     2041 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa

     2101 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca

     2161 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca

     2221 tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat

     2281 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa

     2341 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta

     2401 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca

     2461 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat

     2521 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg

     2581 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc

     2641 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt

     2701 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca

     2761 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg

     2821 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt

     2881 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca

     2941 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt

     3001 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt

     3061 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg

     3121 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt

     3181 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg

     3241 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag

     3301 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt

     3361 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta

     3421 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg

     3481 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt

     3541 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat

     3601 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg

     3661 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca

     3721 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc

     3781 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc

     3841 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat

     3901 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc

     3961 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga

     4021 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt

     4081 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga

     4141 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa

     4201 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac

     4261 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct

     4321 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct

     4381 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg

     4441 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc

     4501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc

     4561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt

     4621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac

     4681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct

     4741 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact

     4801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg