• 规格：10μl质粒
• 原核抗性：卡那霉素Kan
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：          pEGFP-H37RV质粒
• 质粒图谱1： png/1-1G126110519426.png

质粒序列： 点击查看序列

LOCUS       Exported                5201 bp ds-DNA     circular SYN 01-NOV-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5201)

  AUTHORS   admin

  TITLE     Direct Submission

    http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..5201

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        61..364

                     /label=CMV enhancer

                     /note="CMV enhancer;human cytomegalovirus immediate early 

                     enhancer"

     promoter        365..568

                     /label=CMV promoter

                     /note="CMV promoter;human cytomegalovirus (CMV) immediate 

                     early promoter"

     misc_feature    634..1113

                     /label=H37Rv

     CDS             1147..1866

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="EGFP;mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     polyA_signal    1989..2110

                     /label=SV40 poly(A) signal

                     /note="SV40 poly(A) signal;SV40 polyadenylation signal"

     rep_origin      complement(2117..2572)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 ori;f1 bacteriophage origin of replication; arrow

                     indicates direction of (+) strand synthesis"

     promoter        2599..2703

                     /gene="bla"

                     /label=bla AmpR promoter

                     /note="AmpR promoter"

     promoter        2705..3062

                     /label=SV40 promoter

                     /note="SV40 promoter;SV40 enhancer and early promoter"

     rep_origin      2913..3048

                     /label=SV40 ori

                     /note="SV40 ori;SV40 origin of replication"

     CDS             3097..3891

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="NeoR/KanR;confers resistance to neomycin, kanamycin,

                     and G418 (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    4123..4170

                     /label=HSV TK poly(A) signal

                     /note="HSV TK poly(A) signal;herpesvirus thymidine kinase 

                     polyadenylation signal"

     rep_origin      4499..5087

                     /direction=RIGHT

                     /label=ori

                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

ORIGIN

        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

      601 ccggactcag atctcgagct caagcttacc gccatgagct ggtcgcgggt gatcgcctac

      661 gggctgctgc ccgggctggc gttggcgctg acgtgtggcg cgggcttgct gaaatggcag

      721 gacggcgccg tccgcgacgc cgcggttgcc cgtgcggaat ccgtgcgggc cgcgaccgac

      781 ggcaccaccg cgctgctgtc ttaccggccc gacaccgtgc agcatgacct cgagagcgcg

      841 cgaagcaggc tcacgggcac gttcctcgac gcctacacac agctgaccca cgacgtggtg

      901 atccccggcg cacagcagaa gcagatctcg gccgtggcca ccgtcgcggc cgcggcgtcg

      961 gtgtcgactt ccgccgaccg cgccgtcgtc ctgctgttcg taaaccagac catcaccgtc

     1021 ggcaaggacg cgccgaccac cgccgcttcc agcgttcggg tgaccctcga caacatcaac

     1081 gggcgttggc tgatctcgca attcgaaccg atcggaggag gagggtcgga tccaccggtc

     1141 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag

     1201 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc

     1261 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg

     1321 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac

     1381 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc

     1441 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac

     1501 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg

     1561 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag

     1621 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag

     1681 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac

     1741 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac

     1801 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac

     1861 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac

     1921 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg

     1981 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa

     2041 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca

     2101 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt

     2161 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca

     2221 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta

     2281 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta

     2341 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg

     2401 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga

     2461 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg

     2521 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc

     2581 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat

     2641 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag

     2701 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc

     2761 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt

     2821 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt

     2881 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg

     2941 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct

     3001 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca

     3061 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac

     3121 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca

     3181 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt

     3241 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg

     3301 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga

     3361 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct

     3421 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg

     3481 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg

     3541 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc

     3601 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat

     3661 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac

     3721 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt

     3781 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct

     3841 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc

     3901 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca

     3961 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga

     4021 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg

     4081 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga

     4141 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc

     4201 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt

     4261 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg

     4321 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt

     4381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt

     4441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt

     4501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag

     4561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca

     4621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca

     4681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg

     4741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg

     4801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct

     4861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga

     4921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc

     4981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg

     5041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg

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