• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：嘌呤霉素Puro
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：                      pLVX-TetOne-Puro是一个慢病毒四环素诱导表达质粒。
• 质粒图谱1： png/1-1G203100054242.png

质粒序列： 点击查看序列

LOCUS       Exported                9227 bp ds-DNA     circular SYN 03-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..9227

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     LTR             1..634

                     /label=3' LTR

                     /note="3' long terminal repeat (LTR) from HIV-1"

     misc_feature    681..806

                     /label=HIV-1 Psi

                     /note="packaging signal of human immunodeficiency virus 

                     type 1"

     misc_feature    1303..1536

                     /label=RRE

                     /note="The Rev response element (RRE) of HIV-1 allows for 

                     Rev-dependent mRNA export from the nucleus to the 

                     cytoplasm."

     misc_feature    2028..2143

                     /label=cPPT/CTS

                     /note="central polypurine tract and central termination 

                     sequence of HIV-1 (lacking the first T)"

     polyA_signal    2187..2321

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     misc_feature    2496..2527

                     /label=MCS

     promoter        complement(2528..2895)

                     /label=TRE3GS promoter

                     /note="3rd-generation Tet-responsive promoter that can be 

                     activated by binding of Tet-On(R) 3G, modified to eliminate

                     binding sites for endogenous mammalian transcription 

                     factors"

     promoter        2912..3422

                     /label=hPGK promoter

                     /note="human phosphoglycerate kinase 1 promoter"

     CDS             3441..4187

                     /codon_start=1

                     /product="modified rtTA protein that binds tightly to 

                     promoters containing the tet operator in the presence of 

                     doxycycline"

                     /label=Tet-On 3G

                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV

                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR

                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT

                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA

                     DALDDFDLDMLPADALDDFDLDMLPG"

     promoter        4198..4527

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      4378..4513

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             4536..5135

                     /codon_start=1

                     /gene="pac from Streptomyces alboniger"

                     /product="puromycin N-acetyltransferase"

                     /label=PuroR

                     /note="confers resistance to puromycin"

                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER

                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA

                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS

                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"

     misc_feature    5149..5737

                     /label=WPRE

                     /note="woodchuck hepatitis virus posttranscriptional 

                     regulatory element"

     LTR             5945..6578

                     /label=3' LTR

                     /note="3' long terminal repeat (LTR) from HIV-1"

     rep_origin      complement(7109..7694)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(7865..8725)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(8726..8830)

                     /gene="bla"

                     /label=AmpR promoter

     polyA_signal    8878..9012

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

ORIGIN

        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca

       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac

      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca

      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg

      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag

      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg

      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat

      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga

      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct

      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc

      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag

      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg

      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga

      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg

      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg

      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct

      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat

     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca

     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc

     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag

     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc

     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg

     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc

     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc

     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct

     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa

     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca

     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt

     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt

     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta

     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt

     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct

     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga

     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag

     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac

     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg

     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat

     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg

     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg

     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca

     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat

     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc cgccggcacc ggtgtatacg

     2521 ggaattcttt acgagggtag gaagtggtac ggaaagttgg tataagacaa aagtgttgtg

     2581 gaattgaagt ttactcaaaa aatcagcact cttttatagg cgccctggtt tacataagca

     2641 aagcttatac gttctctatc actgataggg agtaaactgg atatacgttc tctatcactg

     2701 atagggagta aactgtagat acgttctcta tcactgatag ggagtaaact ggtcatacgt

     2761 tctctatcac tgatagggag taaactcctt atacgttctc tatcactgat agggagtaaa

     2821 gtctgcatac gttctctatc actgataggg agtaaactct tcatacgttc tctatcactg

     2881 atagggagta aactcgaggt gataattcca cggggttggg gttgcgcctt ttccaaggca

     2941 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg

     3001 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg

     3061 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct

     3121 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg

     3181 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata

     3241 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg

     3301 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc

     3361 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc

     3421 agggggatca tcgaattacc atgtctagac tggacaagag caaagtcata aactctgctc