• 规格：20μl质粒
• 启动子：CMV
• 原核抗性：卡那霉素Kan
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：         pmCherry-LC3a是一个哺乳细胞人源基因自噬表达质粒。
• 质粒图谱1： png/1-1G22009242X20.png

质粒序列： 点击查看序列

LOCUS       Exported                5080 bp ds-DNA     circular SYN 20-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5080

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        61..364

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        365..568

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     regulatory      607..616

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     CDS             613..1320

                     /codon_start=1

                     /product="monomeric derivative of DsRed fluorescent protein

                     (Shaner et al., 2004)"

                     /label=mCherry

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG

                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF

                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK

                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA

                     EGRHSTGGMDELYK"

     CDS             1342..1704

                     /codon_start=1

                     /gene="MAP1LC3A"

                     /product="human LC3 protein"

                     /label=LC3a

                     /note="Atg8 family member found on autophagosomes"

                     /translation="MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLP

                     VLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKD

                     EDGFLYMVYASQETFGF"

     polyA_signal    1868..1989

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(1996..2451)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2478..2582

                     /gene="bla"

                     /label=AmpR promoter

     promoter        2584..2941

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      2792..2927

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             2976..3770

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    4002..4049

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      4378..4966

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc

      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc

      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt

      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc

      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc

      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc

      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc

     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac

     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc

     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc

     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg

     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag

     1321 tccggactca gatctcgagc tatgccctca gaccggcctt tcaagcagcg gcggagcttc

     1381 gccgaccgct gtaaggaggt acagcagatc cgcgaccagc accccagcaa aatcccggtg

     1441 atcatcgagc gctacaaggg tgagaagcag ctgcccgtcc tggacaagac caagtttttg

     1501 gtcccggacc atgtcaacat gagcgagttg gtcaagatca tccggcgccg cctgcagctg

     1561 aaccccacgc aggccttctt cctgctggtg aaccagcaca gcatggtgag tgtgtccacg

     1621 cccatcgcgg acatctacga gcaggagaaa gacgaggacg gcttcctcta tatggtctac

     1681 gcctcccagg aaaccttcgg cttctgagaa ttctgcagtc gacggtaccg cgggcccggg

     1741 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact

     1801 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt

     1861 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa

     1921 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa

     1981 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt

     2041 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa

     2101 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa

     2161 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac

     2221 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga

     2281 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa

     2341 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc

     2401 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca

     2461 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata

     2521 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga

     2581 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc

     2641 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg

     2701 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc

     2761 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc

     2821 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc

     2881 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa

     2941 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg

     3001 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa

     3061 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg

     3121 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt

     3181 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa

     3241 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc

     3301 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg

     3361 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg

     3421 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg

     3481 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg

     3541 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact

     3601 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg

     3661 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc

     3721 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct

     3781 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac

     3841 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat

     3901 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga

     3961 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa

     4021 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca

     4081 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc

     4141 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc

     4201 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt

     4261 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta

     4321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg

     4381 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc

     4441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag

     4501 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa

     4561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc

     4621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc