• 规格：20μl质粒
• 启动子：CMV
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：          PX405 Neisseria meningitidis Cas9是一个哺乳细胞CAS9表达质粒。

质粒序列： 点击查看序列

> CMV-F 5'-CGCAAATGGGCGGTAGGCGTG-3'

cTaTataaGCaGAgCTGGTTtaGTGAaCCGTGGATCTTAATACGACTCACTATAGGGGAATTGTGAGCGG

ATAACAATTCCGGGACCTTTAATTCAACCCAACACAATATATTATAGTTAAATAAGAATTATTATCAAAT

CATTTGTATATTAATTAAAATACTATACTGTAAATTACATTTTATTTACAATCAACAAGTTTGTACAAAA

AAGCAGGCTAAGGAGATATACCACCGGTGCCACCATGTACCCATACGATGTTCCAGATTACGCTTCGCCG

AAGAAAAAGCGCAAGGTCGAAGCGTCCGCTGCCTTCAAACCTAACCCTATCAACTACATCCTGGGGCTGG

ACATTGGAATCGCTTCAGTGGGCTGGGCTATGGTGGAAATCGACGAGGATGAAAACCCTATTTGCCTGAT

CGACCTGGGGGTCAGAGTGTTTGAGAGGGCTGAAGTGCCAAAGACAGGAGATAGTCTGGCTATGGCACGG

AGACTGGCAAGGTCAGTGAGGCGCCTGACTCGACGGAGAGCACACAGGCTGCTGCGAGCTAGGCGCCTGC

TGAAGCGAGAGGGAGTGCTGCAGGCCGCTGACTTCGATGAAAACGGGCTGATCAAGAGCCTGCCCAATAC

CCCTTGGCAGCTGAGAGCAGCCGCTCTGGACAGGAAGCTGACACCACTGGAGTGGTCTGCCGTGCTGCTG

CACCTGATCAAGCATCGCGGATACCTGAGTCAGCGAAAAAACGAGGGCGAAACAGCAGATAAGGAGCTGG

GAGCCCTGCTGAAAGGCGTGGCTGACAATGCCCACGCTCTGCAGACCGGCGATTTTAGGACACCCGCaGA

ACTGGCCCTGAACAAGTTCGAGAAAGAATCCGGGCACATTCGAAATCAGCGGGGAGACTATTCTCatacc

ttcagtcgca

        

> C-CMV-24 5'-TATTAGGACAAGGCTGGTGGGCAC-3'

ccaggagagGCACTGGGGAGGGGTCACAGGGATGCCACCCGACCACTTTGTACAAGAAAGCTGGGTTACG

ATTTAGGTGACACTATAGGCGGCCGCAAGCTTGAGCTCGGTACCGAATTCTTACCGCACGGGTGGTCGTT

TCTTCAGCCGACATGGTCGAATTTCCTTGCCCAGCTCGTCGATCTGATATTTCTGGAAAGACAGGGCGGT

CTTCACGCCGATTCCCTCCAGAATCCCGTTCTTGCCAATTTTATGATCCAGGTCGTGGATTCGAATATTG

ATGTTCCCTGTGCCGCGATGGCAGGATGCGAAGTACCCAAACATTCTAGCTTTCTTGGTGATGACCTCCA

CCAGATCATTAGGGTGCAGGGAGAACTTAAAGTTGAAGCTATCGTCAATCAGCTGCCAATCTTCCTCGTC

TTTTCCCTGCACGACAGCTCGGTCTGGCAGGATGCCCTTTGCGACCTGCCAGCTGTAGATAGGCACCAGA

TAGTACTTATCGCCTTTCTCAAAGACGTCCACCCGCACCATTGTTGCATTATCAGCGATCCCATTATGGT

TCCTCACCCAGACGCCGGTTTTCTGGACCTGTTCCACCCTGACTGCCTTCACCTGCTGAGTTCTGTTTCC

AGCCTTGTCATATTTGTAGAAGGGCTCAGCAAAGGCTTTTGCTGGATCGTCCTTGTGGGCTTCCAGCCTG

GCTTTCAGTGCCTCATACAGCTTGGGTTCTCTCTCCCGATTGACCATCTTCTCCAGGTCCTTCAGCTTCA

GCTGTGTCAGTGGCACTCTCAGCACAgAGACGCCTTCgTCCAGCCGCTTAgCGGATTTCACGGTCTCCAT

GTGTCCCTGCCCGCTCATCTTGCGGTTAGGTGCCCTTGACACgAACAgggg

        

> NmCas9

ACCGGTGCCACCATGTACCCATACGATGTTCCAGATTACGCTTCGCCGAAGAAAAAGCGCAAGGTCGAAG

CGTCCGCTGCCTTCAAACCTAACCCTATCAACTACATCCTGGGGCTGGACATTGGAATCGCTTCAGTGGG

CTGGGCTATGGTGGAAATCGACGAGGATGAAAACCCTATTTGCCTGATCGACCTGGGGGTCAGAGTGTTT

GAGAGGGCTGAAGTGCCAAAGACAGGAGATAGTCTGGCTATGGCACGGAGACTGGCAAGGTCAGTGAGGC

GCCTGACTCGACGGAGAGCACACAGGCTGCTGCGAGCTAGGCGCCTGCTGAAGCGAGAGGGAGTGCTGCA

GGCCGCTGACTTCGATGAAAACGGGCTGATCAAGAGCCTGCCCAATACCCCTTGGCAGCTGAGAGCAGCC

GCTCTGGACAGGAAGCTGACACCACTGGAGTGGTCTGCCGTGCTGCTGCACCTGATCAAGCATCGCGGAT

ACCTGAGTCAGCGAAAAAACGAGGGCGAAACAGCAGATAAGGAGCTGGGAGCCCTGCTGAAAGGCGTGGC

TGACAATGCCCACGCTCTGCAGACCGGCGATTTTAGGACACCCGCAGAACTGGCCCTGAACAAGTTCGAG

AAAGAATCCGGGCACATTCGAAATCAGCGGGGAGACTATTCTCATACCTTCAGTCGCAAGGATCTGCAGG

CCGAGCTGATCCTGCTGTTTGAGAAGCAGAAAGAGTTCGGAAACCCCCACGTGTCTGGCGGGCTGAAAGA

GGGCATCGAAACACTGCTGATGACTCAGCGGCCTGCACTGAGTGGAGACGCCGTGCAGAAGATGCTGGGC

CATTGTACTTTTGAGCCAGCCGAACCCAAGGCAGCCAAAAATACCTACACAGCTGAGAGGTTCATTTGGC

TGACCAAGCTGAACAATCTGCGCATCCTGGAGCAGGGCAGCGAACGGCCCCTGACTGACACCGAGAGAGC

CACACTGATGGATGAACCTTACAGGAAGTCTAAACTGACTTATGCACAGGCACGAAAGCTGCTGGGCCTG

GAGGACACTGCCTTCTTTAAGGGACTGCGGTACGGCAAAGATAACGCTGAGGCAAGCACCCTGATGGAAA

TGAAGGCTTATCACGCAATCTCCAGAGCCCTGGAGAAAGAAGGCCTGAAGGACAAGAAATCACCCCTGAA

TCTGAGCCCTGAGCTGCAGGATGAAATTGGCACCGCTTTTAGCCTGTTCAAGACTGACGAGGATATCACC

GGGCGCCTGAAAGACAGAATCCAGCCCGAGATCCTGGAAGCCCTGCTGAAGCACATCTCTTTTGATAAAT

TCGTGCAGATTAGTCTGAAGGCTCTGCGACGGATCGTCCCTCTGATGGAGCAGGGCAAAAGATACGACGA

GGCCTGCGCTGAAATCTACGGGGATCATTACGGAAAGAAAAACACAGAGGAAAAAATCTACCTGCCCCCT

ATCCCTGCCGACGAGATTCGGAACCCAGTGGTCCTGAGAGCCCTGTCACAGGCTAGAAAAGTGATCAACG

GGGTGGTCAGAAGATACGGAAGCCCCGCAAGAATCCACATTGAGACTGCCCGCGAAGTGGGGAAGTCCTT

TAAAGACCGCAAGGAGATCGAAAAGCGACAGGAGGAAAACAGAAAAGATAGGGAGAAGGCTGCAGCCAAA

TTCCGGGAATACTTTCCAAATTTCGTGGGAGAGCCCAAGTCAAAAGACATCCTGAAGCTGAGACTGTACG

AGCAGCAGCACGGCAAGTGTCTGTATAGCGGGAAAGAAATTAACCTGGGAAGGCTGAATGAGAAGGGCTA

TGTGGAAATCGATCATGCCCTGCCTTTTAGTCGCACATGGGACGATTCATTCAACAATAAGGTCCTGGTG

CTGGGCAGTGAGAACCAGAACAAGGGGAACCAGACTCCTTACGAATATTTCAACGGCAAGGACAACAGCC

GGGAGTGGCAGGAGTTCAAGGCCCGGGTGGAGACCTCCCGCTTCCCACGATCTAAGAAACAGCGGATTCT

GCTGCAGAAGTTTGACGAGGATGGGTTCAAAGAAAGAAACCTGAATGACACCAGATACGTGAATAGATTT

CTGTGCCAGTTCGTCGCAGATCGCATGCGACTGACAGGAAAAGGCAAGAAACGGGTGTTTGCCAGCAACG

GCCAGATCACTAATCTGCTGAGAGGGTTCTGGGGACTGCGGAAGGTGAGAGCCGAGAACGACAGGCACCA

TGCCCTGGATGCTGTGGTCGTGGCTTGTAGCACTGTGGCAATGCAGCAGAAGATTACCAGGTTTGTCCGC

TATAAAGAGATGAATGCCTTCGACGGGAAGACAATCGATAAAGAGACTGGAGAAGTGCTGCACCAGAAGA

CACATTTTCCTCAGCCCTGGGAGTTCTTTGCCCAGGAAGTGATGATCCGCGTCTTTGGCAAGCCTGACGG

GAAACCAGAGTTCGAGGAAGCTGATACCCCAGAGAAGCTGCGGACACTGCTGGCAGAAAAACTGAGCTCC

AGACCCGAGGCCGTGCACGAATACGTCACCCCCCTGTTCGTGTCAAGGGCACCTAACCGCAAGATGAGCG

GGCAGGGACACATGGAGACCGTGAAATCCGCTAAGCGGCTGGACGAAGGCGTCTCTGTGCTGAGAGTGCC

ACTGACACAGCTGAAGCTGAAGGACCTGGAGAAGATGGTCAATCGGGAGAGAGAACCCAAGCTGTATGAG

GCACTGAAAGCCAGGCTGGAAGCCCACAAGGACGATCCAGCAAAAGCCTTTGCTGAGCCCTTCTACAAAT

ATGACAAGGCTGGAAACAGAACTCAGCAGGTGAAGGCAGTCAGGGTGGAACAGGTCCAGAAAACCGGCGT

CTGGGTGAGGAACCATAATGGGATCGCTGATAATGCAACAATGGTGCGGGTGGACGTCTTTGAGAAAGGC

GATAAGTACTATCTGGTGCCTATCTACAGCTGGCAGGTCGCAAAGGGCATCCTGCCAGACCGAGCTGTCG

TGCAGGGAAAAGACGAGGAAGATTGGCAGCTGATTGACGATAGCTTCAACTTTAAGTTCTCCCTGCACCC

TAATGATCTGGTGGAGGTCATCACCAAGAAAGCTAGAATGTTTGGGTACTTCGCATCCTGCCATCGCGGC

ACAGGGAACATCAATATTCGAATCCACGACCTGGATCATAAAATTGGCAAGAACGGGATTCTGGAGGGAA

TCGGCGTGAAGACCGCCCTGTCTTTCCAGAAATATCAGATCGACGAGCTGGGCAAGGAAATTCGACCATG

TCGGCTGAAGAAACGACCACCCGTGCGGTAAGAATTC