• 规格：20μl质粒
• 原核抗性：卡那霉素Kan
• 筛选标记：红色荧光蛋白mRFP1
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：          RedTrackCMV是一个腺病毒Ad系列表达质粒。
• 质粒图谱1： png/1-1G2200R915L9.png

质粒序列： 点击查看序列

LOCUS       Exported                9126 bp ds-DNA     circular SYN 08-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..9126

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_signal     198..348

                     /label=Ad5 Psi

                     /note="packaging signal for adenovirus serotype 5"

     polyA_signal    491..612

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     CDS             complement(760..1437)

                     /codon_start=1

                     /product="monomeric derivative of DsRed (Campbell et al., 

                     2002)"

                     /label=mRFP1

                     /note="mammalian codon-optimized"

                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK

                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV

                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM

                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS

                     TGA"

     promoter        complement(1480..1683)

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     polyA_signal    2046..2167

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     promoter        complement(2445..2648)

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     enhancer        2649..2952

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     rep_origin      complement(6523..7111)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             7918..8712

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

ORIGIN

        1 ttaattaatc ccttccagct ctctgcccct tttggattga agccaatatg ataatgaggg

       61 ggtggagttt gtgacgtggc gcgggcgtgg gaacggggcg ggtgacgtag tagtgtggcg

      121 gaagtgtgat gttgcaagtg tggcggaaca catgtaagcg acggatgtgg caaaagtgac

      181 gtttttggtg tgcgccggtg tacacaggaa gtgacaattt tcgcgcggtt ttaggcggat

      241 gttgtagtaa atttgggcgt aaccgagtaa gatttggcca ttttcgcggg aaaactgaat

      301 aagaggaagt gaaatctgaa taattttgtg ttactcatag cgcgtaatat ttgtctaggg

      361 ccgcggggac tttgaccgtt tacgtggaga ctcgcccagg tgtttttctc aggtgttttc

      421 cgcgttccgg gtcaaagttg gcgttttatt attatagtca gtcgagtcta gcaagctagc

      481 ttgatcgcgt taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa

      541 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc

      601 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg

      661 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatctt

      721 gtggtatggc tgattatgat ctagagtcgc ggccaattct taggcgccgg tggagtggcg

      781 gccctcggcg cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc

      841 cagcttgatg tcggtcttgt aggcgccggg cagctgcacg ggcttcttgg ccatgtaggt

      901 ggtcttgacc tcggcgtcgt agtggccgcc gtccttcagc ttcagcctca tcttgatctc

      961 gcccttcagg gcgccgtcct cggggtacat ccgctcggtg gaggcctccc agcccatggt

     1021 cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta

     1081 gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc

     1141 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg

     1201 gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac tggaactgag gggacaggat

     1261 gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc

     1321 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc

     1381 ctccatgcgc accttgaagc gcatgaactc cttgatgacg tcctcggagg aggccatcgg

     1441 atcccggtag cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc

     1501 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg

     1561 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact

     1621 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat

     1681 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa

     1741 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg

     1801 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc

     1861 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat

     1921 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta

     1981 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta

     2041 cgcgttaaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg

     2101 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa

     2161 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga

     2221 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc

     2281 tagatccggt ggatctgagt ccggacttgt acagctcgtc catgccgaga gtgatcccgg

     2341 cggcggtcac gaactccaga tcggatatct tatctagaag cttaggctcg agcggccgcg

     2401 tcgacggtac cagatctcta gcggatctga cggttcacta aaccagctct gcttatatag

     2461 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat

     2521 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg

     2581 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac

     2641 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc

     2701 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata

     2761 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa

     2821 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc

     2881 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta

     2941 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac

     3001 tattagacgt ttatcactgc tagaggatct gggcgtggtt aagggtggga aagaatatat

     3061 aaggtggggg tcttatgtag ttttgtatct gttttgcagc agccgccgcc gccatgagca

     3121 ccaactcgtt tgatggaagc attgtgagct catatttgac aacgcgcatg cccccatggg

     3181 ccggggtgcg tcagaatgtg atgggctcca gcattgatgg tcgccccgtc ctgcccgcaa

     3241 actctactac cttgacctac gagaccgtgt ctggaacgcc gttggagact gcagcctccg

     3301 ccgccgcttc agccgctgca gccaccgccc gcgggattgt gactgacttt gctttcctga

     3361 gcccgcttgc aagcagtgca gcttcccgtt catccgcccg cgatgacaag ttgacggctc

     3421 ttttggcaca attggattct ttgacccggg aacttaatgt cgtttctcag cagctgttgg

     3481 atctgcgcca gcaggtttct gccctgaagg cttcctcccc tcccaatgcg gtttaaaaca

     3541 taaataaaaa accagactct gtttggattt ggatcaagca agtgtcttgc tgtctttatt

     3601 taggggtttt gcgcgcgcgg taggcccggg accagcggtc tcggtcgttg agggtcctgt

     3661 gtattttttc caggacgtgg taaaggtgac tctggatgtt cagatacatg ggcataagcc

     3721 cgtctctggg gtggaggtag caccactgca gagcttcatg ctgcggggtg gtgttgtaga

     3781 tgatccagtc gtagcaggag cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc

     3841 tgattgccag gggcaggccc ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt

     3901 gcatacgtgg ggatatgaga tgcatcttgg actgtatttt taggttggct atgttcccag

     3961 ccatatccct ccggggattc atgttgtgca gaaccaccag cacagtgtat ccggtgcact

     4021 tgggaaattt gtcatgtagc ttagaaggaa atgcgtggaa gaacttggag acgcccttgt

     4081 gacctccaag attttccatg cattcgtcca taatgatggc aatgggccca cgggcggcgg

     4141 cctgggcgaa gatatttctg ggatcactaa cgtcatagtt gtgttccagg atgagatcgt

     4201 cataggccat ttttacaaag cgcgggcgga gggtgccaga ctgcggtata atggttccat

     4261 ccggcccagg ggcgtagtta ccctcacaga tttgcatttc ccacgctttg agttcagatg

     4321 gggggatcat gtctacctgc ggggcgatga agaaaacggt ttccggggta ggggagatca

     4381 gctgggaaga aagcaggttc ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa

     4441 tcacacctat taccgggtgc aactggtagt taagagagct gcagctgccg tcatccctga

     4501 gcaggggggc cacttcgtta agcatgtccc tgactcgcat gttttccctg accaaatccg

     4561 ccagaaggcg ctcgccgccc agcgatagca gttcttgcaa ggaagcaaag tttttcaacg

     4621 gtttgagacc gtccgccgta ggcatgcttt tgagcgtttg accaagcagt tccaggcggt

     4681 cccacagctc ggtcacctgc tctacggcat ctcgatccag catatctcct cgtttcgcgg

     4741 gttggggcgg ctttcgctgt acggcagtag tcggtgctcg tccagacggg ccagggtcat

     4801 gtctttccac gggcgcaggg tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc

     4861 tccgggctgc gcgctggcca gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg

     4921 ccggtcttcg ccctgcgcgt cggccaggta gcatttgacc atggtgtcat agtccagccc

     4981 ctccgcggcg tggcccttgg cgcgcagctt gcccttggag gaggcgccgc acgaggggca

     5041 gtgcagactt ttgagggcgt agagcttggg cgcgagaaat accgattccg gggagtaggc

     5101 atccgcgccg caggccccgc agacggtctc gcattccacg agccaggtga gctctggccg

     5161 ttcggggtca aaaaccaggt ttcccccatg ctttttgatg cgtttcttac ctctggtttc

     5221 catgagccgg tgtccacgct cggtgacgaa aaggctgtcc gtgtccccgt atacagactg

     5281 tttaaacgaa ttctataaaa tgcaaggtgc tgctcaaaaa atcaggcaaa gcctcgcgca

     5341 aaaaagaaag cacatcgtag tcatgctcat gcagataaag gcaggtaagc tccggaacca

     5401 ccacagaaaa agacaccatt tttctctcaa acatgtctgc gggtttctgc ataaacacaa

     5461 aataaaataa caaaaaaaca tttaaacatt agaagcctgt cttacaacag gaaaaacaac

     5521 ccttataagc ataagacgga ctacggccat gccggcgtga ccgtaaaaaa actggtcacc

     5581 gtgattaaaa agcaccaccg acagctcctc ggtcatgtcc ggagtcataa tgtaagactc

     5641 ggtaaacaca tcaggttgat tcatcggtca gtgctaaaaa gcgaccgaaa tagcccgggg

     5701 gaatacatac ccgcaggcgt agagacaaca ttacagcccc cataggaggt ataacaaaat

     5761 taataggaga gaaaaacaca taaacacctg aaaaaccctc ctgcctaggc aaaatagcac

     5821 cctcccgctc cagaacaaca tacagcgctt cacagcggca gcctaacagt cagccttacc

     5881 agtaaaaaag aaaacctatt aaaaaaacac cactcgacac ggcaccagct caatcagtca

     5941 cagtgtaaaa aagggccaag tgcagagcga gtatatatag gactaaaaaa tgacgtaacg

     6001 gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca gaaacg