• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DB3.1
• 质粒简介：         pAd-PL-DEST pAd/PL-DEST是一个腺病毒Ad系列Gateway系统质粒。
• 质粒图谱1： png/1-1G2200R12N30.png

质粒序列： 点击查看序列

LOCUS       Exported               34864 bp ds-DNA     circular SYN 20-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..34864

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     repeat_region   1..103

                     /label=ITR

                     /note="inverted terminal repeat of human adenovirus 

                     serotype 5"

     misc_signal     191..341

                     /label=Ad5 Psi

                     /note="packaging signal for adenovirus serotype 5"

     protein_bind    512..636

                     /gene="mutant version of attR"

                     /label=attR1

                     /bound_moiety="LR Clonase(TM)"

                     /note="recombination site for the Gateway(R) LR reaction"

     promoter        661..691

                     /label=lac UV5 promoter

                     /note="E. coli lac promoter with an ""up"" mutation"

     CDS             745..1404

                     /codon_start=1

                     /gene="cat"

                     /product="chloramphenicol acetyltransferase"

                     /label=CmR

                     /note="confers resistance to chloramphenicol"

                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL

                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS

                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM

                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"

     CDS             1746..2051

                     /codon_start=1

                     /gene="ccdB"

                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"

                     /label=ccdB

                     /note="Plasmids containing the ccdB gene cannot be 

                     propagated in standard E. coli strains."

                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK

                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"

     protein_bind    complement(2092..2216)

                     /gene="mutant version of attR"

                     /label=attR2

                     /bound_moiety="LR Clonase(TM)"

                     /note="recombination site for the Gateway(R) LR reaction"

     misc_feature    5766..5869

                     /label=Ad SA

                     /note="adenovirus major late transcript splice acceptor 

                     (Friedrich and Soriano, 1991)"

     CDS             complement(12964..12975)

                     /codon_start=1

                     /product="Factor Xa recognition and cleavage site"

                     /label=Factor Xa site

                     /translation="IEGR"

     regulatory      20448..20457

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     repeat_region   32680..32782

                     /label=ITR

                     /note="inverted terminal repeat of human adenovirus 

                     serotype 5"

     rep_origin      complement(33006..33594)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(33746..34606)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPA?MATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(34607..34711)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt

       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt

      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg

      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag

      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga

      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg

      361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc

      421 cgggtcaaag ttggcgtttt attattatag tcagtcgaag cttggatccg gtacctctag

      481 aattctcgag cggccgctag cgacatcgat cacaagtttg tacaaaaaag ctgaacgaga

      541 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac

      601 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc

      661 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccggcgaga

      721 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat

      781 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc

      841 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag

      901 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa

      961 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac

     1021 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat

     1081 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc

     1141 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt

     1201 ttcaccagtt ttgatttaaa cgtggccaat atggacaatt tcttcgcccc cgttttcacc

     1261 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat

     1321 catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc

     1381 gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaaac cagataacag

     1441 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga

     1501 agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt gacagcgaca

     1561 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat

     1621 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat

     1681 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg

     1741 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg

     1801 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg

     1861 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg

     1921 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag

     1981 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct

     2041 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac

     2101 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat

     2161 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatc

     2221 gattcgacag atcactgaaa tgtgtgggcg tggcttaagg gtgggaaaga atatataagg

     2281 tgggggtctt atgtagtttt gtatctgttt tgcagcagcc gccgccgcca tgagcaccaa

     2341 ctcgtttgat ggaagcattg tgagctcata tttgacaacg cgcatgcccc catgggccgg

     2401 ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc ccgcaaactc

     2461 tactaccttg acctacgaga ccgtgtctgg aacgccgttg gagactgcag cctccgccgc

     2521 cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact gactttgctt tcctgagccc

     2581 gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat gacaagttga cggctctttt

     2641 ggcacaattg gattctttga cccgggaact taatgtcgtt tctcagcagc tgttggatct

     2701 gcgccagcag gtttctgccc tgaaggcttc ctcccctccc aatgcggttt aaaacataaa

     2761 taaaaaacca gactctgttt ggatttggat caagcaagtg tcttgctgtc tttatttagg

     2821 ggttttgcgc gcgcggtagg cccgggacca gcggtctcgg tcgttgaggg tcctgtgtat

     2881 tttttccagg acgtggtaaa ggtgactctg gatgttcaga tacatgggca taagcccgtc

     2941 tctggggtgg aggtagcacc actgcagagc ttcatgctgc ggggtggtgt tgtagatgat

     3001 ccagtcgtag caggagcgct gggcgtggtg cctaaaaatg tctttcagta gcaagctgat

     3061 tgccaggggc aggcccttgg tgtaagtgtt tacaaagcgg ttaagctggg atgggtgcat

     3121 acgtggggat atgagatgca tcttggactg tatttttagg ttggctatgt tcccagccat

     3181 atccctccgg ggattcatgt tgtgcagaac caccagcaca gtgtatccgg tgcacttggg

     3241 aaatttgtca tgtagcttag aaggaaatgc gtggaagaac ttggagacgc ccttgtgacc

     3301 tccaagattt tccatgcatt cgtccataat gatggcaatg ggcccacggg cggcggcctg

     3361 ggcgaagata tttctgggat cactaacgtc atagttgtgt tccaggatga gatcgtcata

     3421 ggccattttt acaaagcgcg ggcggagggt gccagactgc ggtataatgg ttccatccgg

     3481 cccaggggcg tagttaccct cacagatttg catttcccac gctttgagtt cagatggggg

     3541 gatcatgtct acctgcgggg cgatgaagaa aacggtttcc ggggtagggg agatcagctg

     3601 ggaagaaagc aggttcctga gcagctgcga cttaccgcag ccggtgggcc cgtaaatcac

     3661 acctattacc gggtgcaact ggtagttaag agagctgcag ctgccgtcat ccctgagcag

     3721 gggggccact tcgttaagca tgtccctgac tcgcatgttt tccctgacca aatccgccag

     3781 aaggcgctcg ccgcccagcg atagcagttc ttgcaaggaa gcaaagtttt tcaacggttt

     3841 gagaccgtcc gccgtaggca tgcttttgag cgtttgacca agcagttcca ggcggtccca

     3901 cagctcggtc acctgctcta cggcatctcg atccagcata tctcctcgtt tcgcgggttg

     3961 gggcggcttt cgctgtacgg cagtagtcgg tgctcgtcca gacgggccag ggtcatgtct

     4021 ttccacgggc gcagggtcct cgtcagcgta gtctgggtca cggtgaaggg gtgcgctccg

     4081 ggctgcgcgc tggccagggt gcgcttgagg ctggtcctgc tggtgctgaa gcgctgccgg

     4141 tcttcgccct gcgcgtcggc