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- 规格：20μl质粒
- 原核抗性：氨苄青霉素Amp
- 克隆菌株：大肠杆菌DH5α
- 质粒简介： pRK5-HA-Ubiquitin-WT是一个哺乳细胞人源泛素化质粒。
- 质粒图谱1： png/1-1G21915133b23.png
质粒序列： 点击查看序列LOCUS Exported 4997 bp ds-DNA circular SYN 19-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..4997/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 14..393/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 394..597/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 828..846/label=SP6 promoter/note="promoter for bacteriophage SP6 RNA polymerase"regulatory 963..972/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"CDS 975..1001/codon_start=1/product="HA (human influenza hemagglutinin) epitope tag"/label=HA/translation="YPYDVPDYA"CDS 1032..1259/codon_start=1/gene="Homo sapiens UBC"/product="human ubiquitin C"/label=ubiquitin/translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"polyA_signal 1309..1443/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"promoter 1512..1869/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 1720..1855/label=SV40 ori/note="SV40 origin of replication"rep_origin 2118..2573/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 2855..2959/gene="bla"/label=AmpR promoterCDS 2960..3820/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"rep_origin 3991..4580/direction=RIGHT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"ORIGIN1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc901 acctcggttc tatcgattga attcccgaac cgacagtcgg tctcttcacc aaggccattc961 gcgccaccat gggctacccc tatgatgtgc ctgactacgc agatctcaat ggtggtggtg1021 gtgggtcgac catgcagatc ttcgtgaaga ccctgactgg taagaccatc actctcgaag1081 tggagccgag tgacaccatt gagaatgtca aggcaaagat ccaagacaag gaaggcatcc1141 ctcctgacca gcagaggttg atctttgctg ggaaacagct ggaagatgga cgcaccctgt1201 ctgactacaa catccagaaa gagtccaccc tgcacctggt cctccgtctc agaggtggtt1261 gagcggccgc taagtaagta aggatcccca gcttggccgc catggcccaa cttgtttatt1321 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt1381 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg1441 atcgggaatt aattcggcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg1501 ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga1561 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca1621 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc1681 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc1741 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag1801 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc1861 ttttgcaaaa agctgttaac agcttggcac tggccgtcgt tttacaacgt cgtgactggg1921 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc1981 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg2041 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac2101 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt2161 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt2221 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc2281 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga2341 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc2401 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg2461 ctattctttt aatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct2521 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg2581 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac2641 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt2701 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag2761 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc2821 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt2881 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata2941 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt3001 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc3061 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat3121 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct3181 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca3241 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg3301 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa3361 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg3421 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga3481 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg3541 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt3601 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg3661 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc3721 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca3781 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc3841 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat3901 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc3961 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg4021 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct4081 accaactctt tttccgaagg taactggctt cagcagagcg cagataacca aatactgttc4141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc4201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg4261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt4321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg4381 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg4441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt4501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag4561 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt4621 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta4681 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt4741 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc4801 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca4861 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc4921 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg4981 acatgattac gaattaa//
