• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：嘌呤霉素Puro
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：         pSilencer 4.1-CMV puro-Stuffer是一个哺乳细胞干扰RNA表达质粒。
• 质粒图谱1： png/1-1G219150514958.png

质粒序列： 点击查看序列

LOCUS       Exported                6679 bp ds-DNA     circular SYN 19-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..6679

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        complement(1586..1789)

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     enhancer        1790..2093

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     rep_origin      complement(2685..3273)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3444..4304)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(4305..4409)

                     /gene="bla"

                     /label=AmpR promoter

     polyA_signal    4497..4578

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     CDS             complement(4808..5407)

                     /codon_start=1

                     /gene="pac from Streptomyces alboniger"

                     /product="puromycin N-acetyltransferase"

                     /label=PuroR

                     /note="confers resistance to puromycin"

                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER

                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA

                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS

                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"

     promoter        complement(5504..5820)

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      5518..5653

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     misc_feature    6235..6293

                     /label=CMV pA signal

     misc_feature    6299..1564

                     /label=Stuffer

ORIGIN

        1 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga

       61 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt

      121 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg

      181 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact

      241 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga

      301 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt

      361 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat

      421 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc

      481 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa

      541 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc

      601 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc

      661 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata

      721 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata

      781 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta

      841 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc

      901 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc

      961 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct

     1021 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg

     1081 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga

     1141 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt

     1201 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga

     1261 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc

     1321 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa

     1381 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca

     1441 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc

     1501 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg

     1561 ataaggatcc acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc

     1621 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat

     1681 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg

     1741 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag

     1801 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg

     1861 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat

     1921 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc

     1981 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc

     2041 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact

     2101 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctaagatctg

     2161 gtaccttgaa ttcatgcttc tcctcccttt agtgagggta attctctctc tctccctata

     2221 gtgagtcgta ttaattcctt ctcttctata gtgtcaccta aatcgttgca attcgtaatc

     2281 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga

     2341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt

     2401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga

     2461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc

     2521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg

     2581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc

     2641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc

     2701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga

     2761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc

     2821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat

     2881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg

     2941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc

     3001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga

     3061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact

     3121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt

     3181 ggtagctctt gatccggcaa aaaaaccacc gctggtagcg gtggtttttt tgtttgcaag

     3241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg

     3301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa

     3361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata

     3421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg

     3481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata

     3541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg

     3601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct

     3661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt

     3721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc

     3781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga

     3841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt

     3901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc

     3961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa

     4021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca

     4081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca

     4141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct

     4201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc

     4261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa

     4321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt

     4381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc

     4441 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gagattgcag

     4501 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata

     4561 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg

     4621 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat

     4681 gatcctctag agtcggtggg cctcgggggc gggtgcgggg tcggcggggc cgccccgggt

     4741 ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg

     4801 gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct

     4861 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg

     4921 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga

     4981 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc

     5041 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca

     5101 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt

     5161 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct

     5221 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga

     5281 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga

     5341 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact

     5401 cggtcatgga aggtcgtctc cttgtgaggg gtcaggggcg tgggtcaggg gatggtggcg

     5461 gcaccggtcg tggcggccga cctgcaggca tgcaagctag ctttttgcaa aagcctaggc

     5521 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct

     5581 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag

     5641 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca

     5701 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact

     5761 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc

     5821 aatctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc