• 规格：20μl质粒
• 启动子：TEF1
• 复制子：pUC
• 原核抗性：氨苄青霉素Amp
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：         pTEF:ATP  pTEF-ATP是一个酵母ATP传感器质粒。HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
• 质粒图谱1： png/1-1G226095929204.png

质粒序列： 点击查看序列

LOCUS       Exported                8554 bp ds-DNA     circular SYN 26-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..8554

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    281..1187

                     /label=HO up

     terminator      1205..1394

                     /gene="S. cerevisiae CYC1"

                     /label=CYC1 terminator

                     /note="transcription terminator for CYC1"

     CDS             complement(1427..3262)

                     /codon_start=1

                     /label=ATeam1.03

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK

                     AHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL

                     EFVTAAIDMKTVKVNITTPDGPVYDADIEMVSVRAESGDLGILPGHIPTKAPLKIGAVR

                     LKKDGQTEMVAVSGGTVEVRPDHVTINAQAAETAEGIDKERAEAARQRAQERLNSQSDD

                     TDIRRAELALQRALNRLDVAGKANEFMDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQ

                     SKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELD

                     GDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHM

                     KQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNIL

                     GHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIE"

     promoter        complement(3280..3678)

                     /gene="S. cerevisiae TEF1"

                     /label=TEF1 promoter

                     /note="promoter for EF-1-alpha"

     promoter        3761..4104

                     /label=TEF promoter

                     /note="Ashbya gossypii TEF promoter"

     CDS             4105..4914

                     /codon_start=1

                     /gene="aph(3')-Ia"

                     /product="aminoglycoside phosphotransferase"

                     /label=KanR

                     /note="confers resistance to kanamycin"

                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP

                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA

                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD

                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD

                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"

     terminator      4920..5117

                     /label=TEF terminator

                     /note="Ashbya gossypii TEF terminator"

     misc_feature    5178..5675

                     /label=HO down

     promoter        6639..6743

                     /gene="bla"

                     /label=AmpR promoter

     CDS             6744..7604

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      7775..8363

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt

       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta

      121 ggcaccccag gctttacact ttatgcttcc gcggctcgta tgttgtgtgg aattgtgagc

      181 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccactag tccgaggcct

      241 cgagatccga tatcgccgtg gcggccgcca gctgaagctt aattatcctg ggcacgagtg

      301 aaacaaagct aaaaccttta tttagcatgg ccattgaatg taacaattat atatatcgca

      361 agcacaaaaa atcaaggaga gagaactacc actttgttca tgtgtacaat gttcattatc

      421 tccataagca aaaaaaaaaa aatagaaaac atatgctata aggttgatat tctcacgagt

      481 aagcggcact tgctacttat tgacattgca gatttttggc tacagaaata gtatattaga

      541 gattataatt gctaatcaaa tcaaaatata aaattagtaa accaaaccat ttataccctt

      601 ccttagtagt tatggattgt tttttaatga tatttctgca aaccaaagaa agattgttat

      661 ccagatagaa tttagttttg atattcattt ttttgttgaa gattgaacgc catatctggg

      721 cctcataatt caaaagacgg tgccattatc ggtagcgttt cgcattgtac tggatttcag

      781 aaatttcaca gttgatgaat cgaaaagaat ggtctcattg caacacgtaa ggttaagatg

      841 tcccttttta ccattatagg caataaatga atcataaaac gaccgtatac tggtgaaata

      901 gtagggagaa cgagtacctg tagtaaaaag tataaatcat agttaatcgg gcaatgtccc

      961 tcgatcaagg agtattgtgt catgttcgag acaaacgcca acatttttgt ttcttttgga

     1021 caaatgttgt ttgcatttat gatccgttat attttgatct aatgtagagt tgcacgtagt

     1081 tcttactggc aaagaaatcg atgcatacca aaaaagaata aaggtgatat ttgatcttta

     1141 ccgtttagtt ccaacgtaaa attgtgcctt tggacttaaa atggcgtcgt acgctgagct

     1201 ggatcttcga gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt

     1261 acacgcgtct gtacagaaaa aaaagaaaaa tttgaaatat aaataacgtt cttaatacta

     1321 acataactat aaaaaaataa atagggacct agacttcagg ttgtctaact ccttcctttt

     1381 cggttagagc ggatcttagc tagccgcggt accaagctta ctcgagtcat tcaatgttat

     1441 gtctaatttt gaagttggcc ttgataccat ttttttgttt gtcagcagtg atgtagacgt

     1501 tgtgagagtt ataattgtat tctaacttat gacctaaaat gttaccatct tctttaaaat

     1561 caataccttt caattcaatt ctattgacca aagtgtcacc ttcaaatttt acttcagctc

     1621 tggtcttgta attaccatcg tccttaaaga aaatagttct ttcttggaca taaccttcag

     1681 gcattgcaga cttaaaaaag tcatgttgct tcatgtggtc aggatatcta gcgaagcatt

     1741 gtaaaccgta acccaatgtt gtaactaatg taggccaagg aacaggtaac ttaccggtag

     1801 tgcatatcaa tttcaaagtc aatttaccgt atgttgcgtc accttcacct tcaccactaa

     1861 ctgaaaactt atgaccattg acgtcaccgt ctaattctac taaaataggg acgacacctg

     1921 tgaataattc ttcaccttta ctaaccatac caccagaacc acccttgtat aattcatcca

     1981 tacccaaagt aataccggca gcggtgacaa attctaataa aaccatatga tctctttttt

     2041 cgttaggatc tttggacaac ttagattggt aggacaaata gtgattatca ggtaataata

     2101 caggaccgtc accgataggt gtgttttgtt gatagtggtc tgctaattga acaccaccgt

     2161 ccatgaattc gttagcctta ccagcaacat ccaatctgtt caaagctctt tgtaaagcca

     2221 attctgctct tctgatatcg gtatcatcag attgagagtt caatctttct tgggctcttt

     2281 gtctagcagc ttcagctctt tctttgtcaa taccttcagc tgtttcagca gcttgagcgt

     2341 taatagtaac atgatctggt ctaacttcaa cagtaccacc tgaaactgca accatttctg

     2401 tttgaccgtc ctttttcaat ctaacggcac caattttcaa tggggctttt gtaggaatat

     2461 gacctggcaa aatacccaaa tcaccagatt cagctctgac agaaaccatt tcaatatcgg

     2521 catcgtaaac aggaccatct ggagtagtaa tgttaacctt aacggtcttc atatcgatag

     2581 cagcagtaac aaattccaac aagaccatgt gatctctttt ttcgtttggg tccttagaca

     2641 atttggattg ggtagacaag taatggttat ctggcaacaa aactggacca tcaccaattg

     2701 gagtgttttg ttggtaatga tcagccaatt gtacaccacc atcttcgatg ttgtgtctga

     2761 tcttgaaatg agccttaata ccgttctttt gcttatcggc ggtaatgtaa acgttatggg

     2821 agatgtagtt gtattccaac ttgtgaccca agatgttacc atcttctttg aagtcgatac

     2881 ccttcaattc gattctgtta accaaggtat caccttcgaa cttaacttcg gctctagttt

     2941 tgtagttacc gtcatccttg aagaagatgg ttctttcttg aacgtaacct tctggcatag

     3001 cggatttgaa gaaatcgtgt tgcttcatat gatctgggta tctagcaaaa cattgaacac

     3061 cccaagtcaa agtagtaacc aaagttggcc atggaactgg caatttacct gtggtacaga

     3121 tgaacttcaa ggtcaattta ccataagtag catcaccttc accttcacca gaaacagaaa

     3181 atctgtgacc gttaacatca ccatccaatt caaccaagat tggaacaaca ccagtgaata

     3241 attcttcacc cttagaaacc attttaataa cctaggaaac ttagattaga ttgctatgct

     3301 ttctttctaa tgagcaagaa gtaaaaaaag ttgtaataga acaagaaaaa tgaaactgaa

     3361 acttgagaaa ttgaagaccg tttattaact taaatatcaa tgggaggtca tcgaaagaga

     3421 aaaaaatcaa aaaaaaaatt ttcaagaaaa agaaacgtga taaaaatttt tattgccttt

     3481 ttcgacgaag aaaaagaaac gaggcggtct cttttttctt ttccaaacct ttagtacggg

     3541 taattaacga caccctagag gaagaaagag gggaaattta gtatgctgtg cttgggtgtt

     3601 ttgaagtggt acggcgatgc gcggagtccg agaaaatctg gaagagtaaa aaaggagtag

     3661 aaacattttg aagctatggt gtggaattcc agctgcgggt taattaaggc gcgccagatc

     3721 tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac

     3781 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat

     3841 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc

     3901 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac

     3961 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt

     4021 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca

     4081 catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga

     4141 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg

     4201 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg

     4261 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg

     4321 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca

     4381 tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga agaatatcct

     4441 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt

     4501 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca

     4561 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct

     4621 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc

     4681 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt

     4741 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac

     4801 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat

     4861 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagta

     4921 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg