• 规格：20μl质粒
• 启动子：CAG
• 复制子：pUC
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：        T7opt in pCAGGS(T7 RNA polymerase)是一个T7 RNA 聚合酶表达质粒。
• 质粒图谱1： png/1-1G226093J6437.png

质粒序列： 点击查看序列

LOCUS       Exported                7381 bp ds-DNA     circular SYN 26-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..7381

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             1725..4376

                     /codon_start=1

                     /label=T7 RNA polymerase

                     /translation="MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYE

                     MGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQ

                     FLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFK

                     KNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIE

                     STGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGI

                     TGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANV

                     ITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLE

                     FMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYY

                     WLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFE

                     YAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAK

                     KVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVM

                     TLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVE

                     AMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLG

                     QFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHD

                     SFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLN

                     LRDILESDFAFA"

     polyA_site      4450..4505

                     /label=-beta-globin poly(A) signal

                     /note="rabbit beta-globin polyadenylation signal"

     promoter        5039..5234

                     /label=SV40 promoter

                     /note="SV40 early promoter"

     rep_origin      5085..5220

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     polyA_signal    5240..5374

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(5613..6201)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(6372..7232)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(7233..7337)

                     /gene="bla"

                     /label=AmpR promoter

     misc_feature    7368..1664

                     /label=CAG promoter

ORIGIN

        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc

       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca

      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt

      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg

      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag

      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt

      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca

      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg

      481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg

      541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg

      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac

      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac

      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt

      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc

      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg

      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg

      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc

     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt

     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag

     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg

     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg

     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg

     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt

     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc

     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt

     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct

     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta

     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc

     1681 tggttattgt gctgtctcat cattttggca aagaattcgc caccatgaac accatcaata

     1741 ttgccaagaa cgacttttct gatatcgagc tggccgctat tccattcaat acactggctg

     1801 accactacgg agagcggctg gcccgcgaac agctggctct ggagcatgaa agctatgaga

     1861 tgggagaagc ccgattcagg aagatgtttg agaggcagct gaaagctggg gaagtggcag

     1921 acaacgcagc cgctaagcca ctgattacca cactgctgcc caaaatgatc gccagaatta

     1981 atgattggtt cgaggaagtg aaggcaaaac gaggaaagag gcctaccgcc ttccagtttc

     2041 tgcaggagat caagccagaa gctgtggcat acatcaccat caagactacc ctggcatgcc

     2101 tgacaagcgc cgacaacaca actgtgcagg cagtcgcttc cgcaatcgga cgagctattg

     2161 aggacgaagc acgctttggg agaatccggg atctggaggc caagcacttc aagaaaaacg

     2221 tggaggaaca gctgaacaag agggtggggc atgtctataa gaaggccttc atgcaggtgg

     2281 tcgaggccga catgctgtca aagggactgc tgggaggaga ggcctggagc tcctggcaca

     2341 aagaagatag catccatgtg ggagtccgct gcatcgagat gctgattgaa tctactggga

     2401 tggtgagtct gcaccgacag aacgccggcg tggtcggaca ggactctgag acaatcgaac

     2461 tggctcccga gtatgccgaa gctattgcaa ctagagccgg agctctggca gggatcagtc

     2521 caatgttcca gccctgcgtg gtccccccta agccttggac tggcatcacc gggggcggat

     2581 actgggctaa tggaaggaga ccactggcac tggtgcgaac acactctaag aaagccctga

     2641 tgagatacga ggatgtctat atgcccgaag tgtataaggc catcaacatt gctcagaata

     2701 cagcatggaa aattaacaag aaagtgctgg ccgtcgctaa tgtgatcact aagtggaaac

     2761 attgtcccgt ggaggacatc cctgccattg aacgggagga actgcctatg aagccagagg

     2821 acatcgatat gaaccctgaa gctctgaccg catggaagcg cgcagccgct gcagtgtaca

     2881 gaaaggataa agcccggaag tcccggcgca tttctctgga gttcatgctg gaacaggcca

     2941 acaagtttgc taatcacaaa gcaatctggt tcccctacaa catggactgg cgcggacgag

     3001 tctatgccgt gagcatgttc aaccctcagg ggaatgatat gacaaagggc ctgctgactc

     3061 tggctaaggg gaaaccaatt gggaaggagg gctactattg gctgaaaatc cacggggcca

     3121 attgcgctgg cgtcgacaag gtgccattcc ccgagaggat caagttcatc gaggaaaacc

     3181 atgaaaatat tatggcatgt gccaagtctc ccctggagaa cacatggtgg gccgaacagg

     3241 atagtccttt ctgctttctg gccttctgtt ttgagtacgc tggagtgcag caccatgggc

     3301 tgagttataa ttgctccctg ccactggcct ttgacggctc ttgtagtgga atccagcact

     3361 tctccgcaat gctgagggat gaggtcggag gaagagcagt gaacctgctg ccatctgaga

     3421 cagtgcagga catctacggc attgtcgcca agaaagtgaa tgagatcctg caggctgacg

     3481 caattaacgg gactgataat gaggtggtca ccgtcacaga tgaaaacact ggcgagatca

     3541 gcgaaaaggt gaaactggga accaaggccc tggctggaca gtggctggca tacggagtca

     3601 cccgctcagt gacaaagcga agcgtgatga ccctggctta tggcagcaaa gagttcggct

     3661 tcaggcagca ggtgctggaa gacaccatcc agccagccat tgattccgga aaggggctga

     3721 tgtttacaca gcccaaccag gccgctggct acatggccaa gctgatctgg gagtcagtga

     3781 gcgtcacagt ggtcgcagcc gtggaagcta tgaattggct gaagtccgct gcaaaactgc

     3841 tggccgctga ggtgaaggac aagaaaactg gcgaaattct gaggaaaaga tgcgccgtcc

     3901 actgggtgac ccctgatgga ttcccagtgt ggcaggagta taagaaaccc atccagacca

     3961 gactgaacct gatgttcctg ggccagtttc ggctgcagcc tacaatcaac actaataagg

     4021 acagtgagat tgatgctcat aaacaggaat cagggattgc acctaatttt gtgcacagcc

     4081 aggacggctc ccatctgcgg aagactgtgg tctgggctca cgagaaatac ggcatcgaat

     4141 ccttcgcact gattcatgac tcttttggaa ccatcccagc cgatgcagcc aacctgttca

     4201 aggctgtccg cgagactatg gtggacacct acgaaagttg tgatgtgctg gccgacttct

     4261 atgatcagtt tgctgaccag ctgcacgagt cacagctgga taagatgccc gcactgcctg

     4321 ccaaaggcaa cctgaatctg agagacatcc tggagtccga tttcgcattt gcctgactcg

     4381 aggatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga

     4441 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc

     4501 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg

     4561 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa

     4621 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct

     4681 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc

     4741 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag

     4801 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa

     4861 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata

     4921 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta

     4981 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc

     5041 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc

     5101 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg

     5161 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc

     5221 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa

     5281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc

     5341 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc

     5401 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg

     5461 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta

     5521 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc