• 规格：20μl质粒
• 启动子：35S
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：          pUC-SPYNE173是一个植物细胞双分子荧光BIFC质粒。
• 质粒图谱1： png/1-1P1041J604492.png

质粒序列： 点击查看序列

LOCUS       Exported                4410 bp ds-DNA     circular SYN 04-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..4410

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        863..1208

                     /label=CaMV 35S promoter

                     /note="strong constitutive promoter from cauliflower mosaic

                     virus"

     misc_feature    1217..1300

                     /label=MCS

     CDS             1304..1333

                     /codon_start=1

                     /product="Myc (human c-Myc proto-oncogene) epitope tag"

                     /label=Myc

                     /translation="EQKLISEEDL"

     CDS             1334..1852

                     /codon_start=1

                     /product="N-terminal fragment of mVenus for use in 

                     bimolecular fluorescence complementation (BiFC) (Kodama and

                     Hu, 2010)"

                     /label=VN173

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIE"

     terminator      1872..2124

                     /label=NOS terminator

                     /note="nopaline synthase terminator and poly(A) signal"

     promoter        2623..2727

                     /gene="bla"

                     /label=AmpR promoter

     CDS             2728..3588

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      3759..4347

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat

       61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag

      121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac

      181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc

      241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt

      301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca

      361 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg

      421 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg

      481 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca

      541 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga

      601 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag

      661 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg

      721 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa

      781 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag

      841 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc

      901 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg

      961 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg

     1021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc

     1081 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg

     1141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg

     1201 agagaacacg ggggactcta gagttaaccg ggctcaggcc tggcgcgcca ctagtggatc

     1261 catcgatagt actgtcgacc tcgagggtac cgctcccggg atggagcaaa agttgatttc

     1321 tgaggaggat cttatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct

     1381 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg

     1441 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt

     1501 gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc

     1561 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga

     1621 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga

     1681 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa

     1741 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga

     1801 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg agtaggagct

     1861 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg

     1921 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta

     1981 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat

     2041 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg

     2101 cggtgtcatc tatgttacta gatcgggaat tcactggccg tcgttttaca acgtcgtgac

     2161 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc

     2221 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat

     2281 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc

     2341 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac

     2401 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga

     2461 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa

     2521 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata

     2581 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt

     2641 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg

     2701 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt

     2761 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta

     2821 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc

     2881 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa

     2941 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc

     3001 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt

     3061 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact

     3121 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac

     3181 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata

     3241 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta

     3301 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg

     3361 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat

     3421 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt

     3481 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga

     3541 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa

     3601 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag

     3661 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac

     3721 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc

     3781 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat

     3841 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat

     3901 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct

     3961 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt

     4021 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg

     4081 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta

     4141 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg

     4201 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg

     4261 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc

     4321 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg

     4381 gccttttgct ggccttttgc tcacatgttc

//