• 规格：20μl质粒
• 启动子：35S
• 终止子：NOS
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：           pUC-SPYNE(R)173是一个植物细胞双分子荧光BIFC质粒。
• 质粒图谱1： png/1-1P104134H0B0.png

质粒序列： 点击查看序列

LOCUS       Exported                4380 bp ds-DNA     circular SYN 04-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..4380

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        863..1208

                     /label=CaMV 35S promoter

                     /note="strong constitutive promoter from cauliflower mosaic

                     virus"

     CDS             1223..1741

                     /codon_start=1

                     /product="N-terminal fragment of mVenus for use in 

                     bimolecular fluorescence complementation (BiFC) (Kodama and

                     Hu, 2010)"

                     /label=VN173

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIE"

     CDS             1742..1771

                     /codon_start=1

                     /product="Myc (human c-Myc proto-oncogene) epitope tag"

                     /label=Myc

                     /translation="EQKLISEEDL"

     misc_feature    1772..1831

                     /label=MCS

     terminator      1842..2094

                     /label=NOS terminator

                     /note="nopaline synthase terminator and poly(A) signal"

     promoter        2593..2697

                     /gene="bla"

                     /label=AmpR promoter

     CDS             2698..3558

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      3729..4317

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat

       61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag

      121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac

      181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc

      241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt

      301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca

      361 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg

      421 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg

      481 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca

      541 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga

      601 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag

      661 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg

      721 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa

      781 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag

      841 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc

      901 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg

      961 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg

     1021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc

     1081 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg

     1141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg

     1201 agagaacacg ggggactcta gaatggtgag caagggcgag gagctgttca ccggggtggt

     1261 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga

     1321 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa

     1381 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc

     1441 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta

     1501 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt

     1561 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga

     1621 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat

     1681 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga

     1741 ggagcaaaag ttgatttctg aggaggatct tgggcccagg cctactagtg gatccgtcga

     1801 cctcgagggt accgctcccg ggtaggagct cgaatttccc cgatcgttca aacatttggc

     1861 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc

     1921 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat

     1981 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat

     2041 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat

     2101 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat

     2161 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat

     2221 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc

     2281 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct

     2341 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg

     2401 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg

     2461 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc

     2521 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt

     2581 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat

     2641 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg

     2701 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt

     2761 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga

     2821 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa

     2881 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt

     2941 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt

     3001 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc

     3061 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga

     3121 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat

     3181 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct

     3241 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc

     3301 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg

     3361 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc

     3421 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg

     3481 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca

     3541 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta

     3601 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc

     3661 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa

     3721 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca

     3781 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta

     3841 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc

     3901 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca

     3961 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta

     4021 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag

     4081 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt

     4141 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc

     4201 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac

     4261 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac

     4321 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc

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