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- 规格：20μl质粒
- 原核抗性：氨苄青霉素Amp
- 筛选标记：绿色荧光蛋白EGFP
- 克隆菌株：大肠杆菌DH5α
- 质粒简介： pMy-BirA-T2A-eGFP是一个生物素化酶逆转录病毒表达质粒。
- 质粒图谱1： png/1-1P106115632596.png
质粒序列： 点击查看序列LOCUS Exported 6380 bp ds-DNA circular SYN 07-AUG-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..6380/organism="synthetic DNA construct"/mol_type="other DNA"CDS 1090..1506/label=gag (truncated)misc_feature 1516..1890/label=pol regionCDS 1905..2867/codon_start=1/label=BirA/translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"CDS 2868..2921/codon_start=1/label=T2A/translation="EGRGSLLTCGDVEENPGP"CDS 2922..3641/codon_start=1/label=EGFP/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"rep_origin complement(4655..5243)/direction=LEFT/label=oriCDS complement(5407..6267)/codon_start=1/label=AmpR/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(6268..6372)/label=AmpR promoterORIGIN1 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg61 caaggcatgg aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaga121 tggaacagct gaatatgggc caaagcggat atctgtggta agcagttcct gccccggctc181 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag241 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca301 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc361 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg421 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt481 cgcccgggta cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc541 gctgatcctt gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt601 tggaggttcc accgagattt ggagacccca gcccagggac caccgacccc cccgccggga661 ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc721 atctaatgtt tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc781 cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc ctgggagacg tcccaggtcg841 ggggccgttt ttgtggcccg acctgagtcc aaaaagcccg atcgttttgg actctttggt901 gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct aaaacagttc961 ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc gcgtcttgtc1021 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat1081 atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat1141 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc1201 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga1261 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac1321 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag1381 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt1441 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta1501 ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct tgtaaacttc1561 cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca cttacaggct1621 ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca agaacaactg1681 gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt ccgccgacac1741 cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct gaccaccccc1801 accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt gaaggctgcc1861 gaccccgggg gtggaccatc ctctagactg ccggatccgc caccatgaag gataacaccg1921 tgccactgaa attgattgcc ctgttagcga acggtgaatt tcactctggc gagcagttgg1981 gtgaaacgct gggaatgagc cgggcggcta ttaataaaca cattcagaca ctgcgtgact2041 ggggcgttga tgtctttacc gttccgggta aaggatacag cctgcctgag cctatccagt2101 tacttaatgc taaacagata ttgggtcagc tggatggcgg tagtgtagcc gtgctgccag2161 tgattgactc cacgaatcag taccttcttg atcgtatcgg agagcttaaa tcgggcgatg2221 cttgcattgc agaataccag caggctggcc gtggtcgccg gggtcggaaa tggttttcgc2281 cttttggcgc aaacttatat ttgtcgatgt tctggcgtct ggaacaaggc ccggcggcgg2341 cgattggttt aagtctggtt atcggtatcg tgatggcgga agtattacgc aagctgggtg2401 cagataaagt tcgtgttaaa tggcctaatg acctctatct gcaggatcgc aagctggcag2461 gcattctggt ggagctgact ggcaaaactg gcgatgcggc gcaaatagtc attggagccg2521 ggatcaacat ggcaatgcgc cgtgttgaag agagtgtcgt taatcagggg tggatcacgc2581 tgcaggaagc ggggatcaat ctcgatcgta atacgttggc ggccatgcta atacgtgaat2641 tacgtgctgc gttggaactc ttcgaacaag aaggattggc accttatctg tcgcgctggg2701 aaaagctgga taattttatt aatcgcccag tgaaacttat cattggtgat aaagaaatat2761 ttggcatttc acgcggaata gacaaacagg gggctttatt acttgagcag gatggaataa2821 taaaaccctg gatgggcggt gaaatatcct tgcgtagtgc agaaaaagag ggcagaggaa2881 gtcttctaac atgcggtgac gtggaggaga atcccggccc tatggtgagc aagggcgagg2941 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca3001 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt3061 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct3121 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt3181 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact3241 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga3301 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca3361 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca3421 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca3481 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg3541 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg3601 ccgccgggat cactctcggc atggacgagc tgtacaagta aactcgacat ttcgaccggg3661 ccgccagcac agtggggggg ggcccgcgat tagtccaatt tgttaaagac aggatatcag3721 tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc3781 catagataga ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca3841 cctgtaggtt tggcaagcta gcttaagtaa gccattttgc aaggcatgga aaaatacata3901 actgagaata gagaagttca gatcaaggtt aggaacagag agacaggaga atatgggcca3961 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agttggaaca4021 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca4081 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg4141 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag4201 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa4261 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgtatcca4321 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct4381 ctgagtgatt gactacccgt cagcgggggt ctttcattct gcattaatga atcggccaac4441 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc4501 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt4561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg4621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg4681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat4741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta4801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct4861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc4921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa4981 gacacgactt atagccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg5041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag5101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt5161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta5221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc5281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca5341 cctagatcct tttgcggccg gccgcaaatc aatctaaagt atatatgagt aaacttggtc5401 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc5461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc5521 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc5581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc5641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt5701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc5761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa5821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt5881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg5941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc6001 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa6061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt6121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt6181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag6241 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta6301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat6361 aggggttccg cgcacatttc//
