• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：URA3
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：           YEplac195是一个酿酒酵母表达质粒。
• 质粒图谱1： png/1-1P10614313G27.png

质粒序列： 点击查看序列

LOCUS       Exported                5240 bp ds-DNA     circular SYN 06-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5240

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     protein_bind    106..127

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence 

                     of cAMP."

     promoter        142..172

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    180..196

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     primer_bind     204..220

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     CDS             216..536

                     /codon_start=1

                     /gene="lacZ fragment"

                     /product="LacZ-alpha fragment of beta-galactosidase"

                     /label=lacZ-alpha

                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR

                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIIGSYLLPII

                     EF"

     misc_feature    233..289

                     /label=MCS

                     /note="pUC18/19 multiple cloning site"

     primer_bind     complement(290..306)

                     /label=M13 fwd

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     rep_origin      742..2088

                     /label=2u ori

                     /note="yeast 2u plasmid origin of replication"

     CDS             complement(2199..3002)

                     /codon_start=1

                     /gene="S. cerevisiae URA3"

                     /product="orotidine-5'-phosphate decarboxylase, required 

                     for uracil biosynthesis"

                     /label=URA3

                     /note="yeast auxotrophic marker, counterselectable with 

                     5-fluoroorotic acid (5-FOA)"

                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL

                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ

                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE

                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD

                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"

     promoter        complement(3003..3218)

                     /gene="S. cerevisiae URA3"

                     /label=URA3 promoter

     promoter        3334..3438

                     /gene="bla"

                     /label=AmpR promoter

     CDS             3439..4299

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      4470..5058

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca

       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct

      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat

      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc

      241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg

      301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac

      361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac

      421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt

      481 gcggtatttc acaccgcata atcggatcgt acttgttacc catcattgaa ttttgaacat

      541 ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata ataatatata

      601 gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac tattgcatct

      661 attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca

      721 cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga acaaaaatgc

      781 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa

      841 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa

      901 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac

      961 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct

     1021 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc

     1081 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag

     1141 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact

     1201 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc

     1261 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt

     1321 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac

     1381 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt

     1441 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg

     1501 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca

     1561 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata

     1621 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc

     1681 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag aataggaact

     1741 tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc

     1801 tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata

     1861 tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct

     1921 atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg

     1981 gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat

     2041 tggattagtc tcatccttca atgctatcat ttcctttgat attggatcga tccgatgata

     2101 agctgtcaaa catgagaatt gggtaataac tgatataatt aaattgaagc tctaatttgt

     2161 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt

     2221 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt

     2281 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca

     2341 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt

     2401 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag

     2461 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca

     2521 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt

     2581 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt

     2641 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg

     2701 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg

     2761 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca

     2821 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg

     2881 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat

     2941 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac

     3001 atgatttatc ttcgtttcct gcatgttttt gttctgtgca gttgggttaa gaatactggg

     3061 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc

     3121 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aagaaaccga

     3181 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt gaaaagctaa ttcttgaaga

     3241 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct

     3301 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc

     3361 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa

     3421 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt

     3481 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct

     3541 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc

     3601 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta

     3661 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac

     3721 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc

     3781 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac

     3841 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg

     3901 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac

     3961 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc

     4021 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt

     4081 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga

     4141 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc

     4201 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag

     4261 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca

     4321 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc