• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：           pCDNA3.1-HA-UB-KO是一个人源泛素基因哺乳细胞表达质粒，由武汉淼灵生物科技有限公司构建并共享至淼灵质粒平台。
• 质粒图谱1： png/1-1P10G05Pc46.png

质粒序列： 点击查看序列

LOCUS       Exported                5690 bp ds-DNA     circular SYN 07-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5690

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        202..581

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        582..785

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     promoter        830..848

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     CDS             908..934

                     /codon_start=1

                     /product="HA (human influenza hemagglutinin) epitope tag"

                     /label=HA

                     /translation="YPYDVPDYA"

     misc_feature    950..1177

                     /label=UB-KO

     polyA_signal    1257..1481

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      1527..1955

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        1969..2298

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      2149..2284

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             2365..3159

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    3333..3454

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(3905..4490)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(4661..5521)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5522..5626)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt

       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct

      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg

      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta

      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg

      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc

      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa

      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca

      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta

      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt

      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg

      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca

      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca

      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc

      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc

      901 cgccatgtac ccttatgacg tccccgatta cgccagcctg tctagaggca tgcagatctt

      961 cgtgaggacc ctgactggta ggaccatcac tctcgaagtg gagccgagtg acaccattga

     1021 gaatgtcagg gcaaggatcc aagacaggga aggcatccct cctgaccagc agaggttgat

     1081 ctttgctggg aggcagctgg aagatggacg caccctgtct gactacaaca tccagaggga

     1141 gtccaccctg cacctggtcc tccgtctcag aggtgggtga gaattctgca gatatccagc

     1201 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt

     1261 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga

     1321 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag

     1381 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga

     1441 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac

     1501 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg

     1561 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt

     1621 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg

     1681 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga

     1741 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac

     1801 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc

     1861 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa

     1921 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta

     1981 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat

     2041 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc

     2101 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta

     2161 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca

     2221 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga

     2281 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa

     2341 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg

     2401 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct

     2461 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac

     2521 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg

     2581 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg

     2641 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa

     2701 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca

     2761 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt

     2821 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc

     2881 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc

     2941 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg

     3001 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt

     3061 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag

     3121 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa

     3181 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct

     3241 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg

     3301 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt

     3361 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta

     3421 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta

     3481 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca

     3541 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag

     3601 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt

     3661 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc

     3721 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg

     3781 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa

     3841 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg

     3901 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga

     3961 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg

     4021 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg

     4081 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc

     4141 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg

     4201 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca

     4261 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt

     4321 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag

     4381 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg

     4441 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt

     4501 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg

     4561 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta

     4621 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg

     4681 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg

     4741 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc

     4801 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg