• 商品货号：FH1825

• 规格：10μl质粒
• 原核抗性：卡那霉素Kan
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒图谱1： png/1-1P12120092K46.png

质粒序列： 点击查看序列

LOCUS       Exported                6122 bp ds-DNA     circular SYN 21-JAN-2018

FEATURES             Location/Qualifiers

     source          1..6122

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        61..364

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        365..568

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     regulatory      607..616

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     CDS             613..1329

                     /codon_start=1

                     /product="enhanced blue variant of GFP"

                     /label=EBFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     misc_feature    1351..2742

                     /label=PAPK2-c

     polyA_signal    2910..3031

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(3038..3493)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        3520..3624

                     /gene="bla"

                     /label=AmpR promoter

     promoter        3626..3983

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      3834..3969

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             4018..4812

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    5044..5091

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      5420..6008

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg

      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc

      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg

      781 ccctggccca ccctcgtgac caccctgacc cacggcgtgc agtgcttcag ccgctacccc

      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag

      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag

      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac

     1021 atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat catggccgac

     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc

     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg

     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc

     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag

     1321 ctgtacaagt ccggactcag atctcgagct atgatagtgt ttgtcaggtt caactccagc

     1381 catggcttcc cagtggagct tgggttggat gccagcatcc tccagctgaa ggaggcggtt

     1441 gcgcagcgac agggagttcc agctgatcag ctgcgggtga tttttgcagg aagagagctc

     1501 agcaacgacc taacactgca gaattgcgac ctggtccagc aaagcatcgt tcatattgtt

     1561 cagaatctac agaagaatag tgataaggat gagacagaag acaatcatgc tggaggcatt

     1621 ctaaaaacat tggaaagagt acctgaaagt ctaactcgca tagatctcag ttccagcatc

     1681 cttccatcgt tgtctgcagg gttggctgtt attctggata ctaaagaacc caacatttct

     1741 cctccctctg aaaaatcagg tgcagctagt tacaacagct tttatgtttt ttgcaagaat

     1801 ttctgccaag ctgtgaaacc tgggaaactg agggttcgat gcaatgaatg taaacaagga

     1861 acactcactt tggcaagggg cccatcttgc tgggatgatg tgctgattcc aaacagaata

     1921 acaggtgttt gccagtcccc agactgcagt ggaaacgtag cggagtttta ctttaaatgt

     1981 ggagcacacc caacaactga cagtgaaaca tctgtggctt tgaaccttgt tactacaaac

     2041 agtcgcggta tcacatgtat aacatgcacg gatattagga gtcccgtgct tgtgttccag

     2101 tgcatgcatc gccatgtcat ttgcctagat tgtttccatt tgtactgtgt gacaatgttg

     2161 aatgatcgtc agttcatcta tgacccagat cttggctact ccctcccgtg tgtagctggc

     2221 tgtccagact ccttgattaa agaggttcat cacttcagga ttctgggaga agaacagtac

     2281 aaccgctacc agcgctatgg tgcggaggag tgcgtgctgc agatgggtgg cctgctgtgt

     2341 ccaactccca gctgtggcgc aggtctgcta cctgaaccag aagtgaggaa aattgtatgt

     2401 gaaccaggca atggaatagg ctgtgggttt gtgttctgcc gtgaatgtaa agaagaatac

     2461 cacgaaggtg aatgcagctc tttcctcagc acacagggag ctgtggccca aaagggatat

     2521 gtagttgatg aaaatgcagc catgcaagcc cggtgggaag aggcatctaa agaaacaatc

     2581 aagaaaacaa ccaagccatg ccccaactgc catattccag tggagaaaaa tggtggctgc

     2641 atgcacatga aatgtcctcg acctcagtgc aggtttgagt ggtgctggaa ctgtggcctg

     2701 gagtggaaca gaacctgcat gggcaatcac tggtttgact gaaagcttcg aattctgcag

     2761 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata

     2821 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga

     2881 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca

     2941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt

     3001 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg

     3061 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc

     3121 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt

     3181 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc

     3241 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg

     3301 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga

     3361 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg

     3421 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg

     3481 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta

     3541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt

     3601 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc

     3661 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc

     3721 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc

     3781 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc

     3841 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt

     3901 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt

     3961 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg

     4021 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc

     4081 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg

     4141 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa

     4201 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc

     4261 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat

     4321 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg

     4381 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc

     4441 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag

     4501 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc

     4561 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc

     4621 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata

     4681 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc

     4741 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac

     4801 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc

     4861 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt

     4921 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc

     4981 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta

     5041 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg

     5101 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat

     5161 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc

     5221 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt