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- 规格：20μl质粒
- 原核抗性：卡那霉素Kan
- 筛选标记：遗传霉素G418
- 克隆菌株：大肠杆菌DH5α
- 质粒图谱1： png/1-1P12120092K46.png
质粒序列： 点击查看序列LOCUS Exported 6122 bp ds-DNA circular SYN 21-JAN-2018FEATURES Location/Qualifierssource 1..6122/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 61..364/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 365..568/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"regulatory 607..616/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"CDS 613..1329/codon_start=1/product="enhanced blue variant of GFP"/label=EBFP/note="mammalian codon-optimized"/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"misc_feature 1351..2742/label=PAPK2-cpolyA_signal 2910..3031/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(3038..3493)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 3520..3624/gene="bla"/label=AmpR promoterpromoter 3626..3983/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 3834..3969/label=SV40 ori/note="SV40 origin of replication"CDS 4018..4812/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Neo/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 5044..5091/label=HSV TK poly(A) signal/note="herpes simplex virus thymidine kinasepolyadenylation signal (Cole and Stacy, 1985)"rep_origin 5420..6008/direction=RIGHT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"ORIGIN1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg781 ccctggccca ccctcgtgac caccctgacc cacggcgtgc agtgcttcag ccgctacccc841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac1021 atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat catggccgac1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag1321 ctgtacaagt ccggactcag atctcgagct atgatagtgt ttgtcaggtt caactccagc1381 catggcttcc cagtggagct tgggttggat gccagcatcc tccagctgaa ggaggcggtt1441 gcgcagcgac agggagttcc agctgatcag ctgcgggtga tttttgcagg aagagagctc1501 agcaacgacc taacactgca gaattgcgac ctggtccagc aaagcatcgt tcatattgtt1561 cagaatctac agaagaatag tgataaggat gagacagaag acaatcatgc tggaggcatt1621 ctaaaaacat tggaaagagt acctgaaagt ctaactcgca tagatctcag ttccagcatc1681 cttccatcgt tgtctgcagg gttggctgtt attctggata ctaaagaacc caacatttct1741 cctccctctg aaaaatcagg tgcagctagt tacaacagct tttatgtttt ttgcaagaat1801 ttctgccaag ctgtgaaacc tgggaaactg agggttcgat gcaatgaatg taaacaagga1861 acactcactt tggcaagggg cccatcttgc tgggatgatg tgctgattcc aaacagaata1921 acaggtgttt gccagtcccc agactgcagt ggaaacgtag cggagtttta ctttaaatgt1981 ggagcacacc caacaactga cagtgaaaca tctgtggctt tgaaccttgt tactacaaac2041 agtcgcggta tcacatgtat aacatgcacg gatattagga gtcccgtgct tgtgttccag2101 tgcatgcatc gccatgtcat ttgcctagat tgtttccatt tgtactgtgt gacaatgttg2161 aatgatcgtc agttcatcta tgacccagat cttggctact ccctcccgtg tgtagctggc2221 tgtccagact ccttgattaa agaggttcat cacttcagga ttctgggaga agaacagtac2281 aaccgctacc agcgctatgg tgcggaggag tgcgtgctgc agatgggtgg cctgctgtgt2341 ccaactccca gctgtggcgc aggtctgcta cctgaaccag aagtgaggaa aattgtatgt2401 gaaccaggca atggaatagg ctgtgggttt gtgttctgcc gtgaatgtaa agaagaatac2461 cacgaaggtg aatgcagctc tttcctcagc acacagggag ctgtggccca aaagggatat2521 gtagttgatg aaaatgcagc catgcaagcc cggtgggaag aggcatctaa agaaacaatc2581 aagaaaacaa ccaagccatg ccccaactgc catattccag tggagaaaaa tggtggctgc2641 atgcacatga aatgtcctcg acctcagtgc aggtttgagt ggtgctggaa ctgtggcctg2701 gagtggaaca gaacctgcat gggcaatcac tggtttgact gaaagcttcg aattctgcag2761 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata2821 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga2881 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca2941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt3001 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg3061 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc3121 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt3181 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc3241 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg3301 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga3361 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg3421 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg3481 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta3541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt3601 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc3661 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc3721 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc3781 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc3841 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt3901 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt3961 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg4021 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc4081 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg4141 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa4201 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc4261 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat4321 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg4381 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc4441 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag4501 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc4561 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc4621 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata4681 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc4741 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac4801 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc4861 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt4921 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc4981 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta5041 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg5101 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat5161 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc5221 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
