• 商品货号：FH1827

• 规格：10μl质粒
• 原核抗性：卡那霉素Kan
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：         参考文献：CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. 
• 质粒图谱1： png/1-1P12119535AR.png

质粒序列： 点击查看序列

LOCUS       Exported               11982 bp ds-DNA     circular SYN 21-JAN-2018

FEATURES             Location/Qualifiers

     source          1..11982

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     rep_origin      2672..2894

                     /label=pSC101 ori

                     /note="low-copy replication origin that requires the Rep101

                     protein"

     CDS             2942..3892

                     /codon_start=1

                     /gene="rep101"

                     /product="RepA protein needed for replication with the 

                     pSC101 origin"

                     /label=Rep101

                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE

                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS

                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI

                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV

                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF

                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"

     terminator      6149..6234

                     /gene="Escherichia coli rrnB"

                     /label=rrnB T1 terminator

                     /note="transcription terminator T1 from the E. coli rrnB 

                     gene"

     terminator      6326..6353

                     /label=rrnB T2 terminator

                     /note="transcription terminator T2 from the E. coli rrnB 

                     gene"

     CDS             complement(6397..10299)

                     /codon_start=1

                     /label=Cpf1

                     /translation="MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAK

                     DYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDT

                     IKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDID

                     EALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLK

                     DKAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKF

                     NTIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVID

                     KLEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSL

                     TDLSQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKL

                     ALEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQA

                     SAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIV

                     PLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNK

                     KNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNH

                     STHTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEF

                     YREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALF

                     DERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDK

                     RFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGERHLAYYTLVD

                     GKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVV

                     HEIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKT

                     GGVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQE

                     FFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTR

                     EVYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTE

                     LDYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNL

                     VIKNEEYFEFVQNRNN"

     CDS             complement(10392..11186)

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Kan

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

ORIGIN

        1 tcctcacgta gtgccaaagg aacgcgtgcg gtgaccacga cgggcttagc ctttgcctgc

       61 gcttctagtg cttcgatggt ggcttgtgcc tgcgcttgct gcgcctgtag tgcctgttga

      121 gcttcttgta gttgctgttc tagctgtgcc ttggttgcca tgctttaaga ctctagtagc

      181 tttcctgcga tatgtcatgc gcatgcgtag caaacattgt cctgcaactc attcattatg

      241 tgcagtgctc ctgttactag tcgtacatac tcatatttac ctagtctgca tgcagtgcat

      301 gcacatgcag tcatgtcgtg ctaatgtgta aaacatgtac atgcagattg ctgggggtgc

      361 agggggcgga gccaccctgt ccatgcgggg tgtggggctt gccccgccgg tacagacagt

      421 gagcaccggg gcacctagtc gcggataccc cccctaggta tcggacacgt aaccctccca

      481 tgtcgatgca aatctttaac attgagtacg ggtaagctgg cacgcatagc caagctaggc

      541 ggccaccaaa caccactaaa aattaatagt ccctagacaa gacaaacccc cgtgcgagct

      601 accaactcat atgcacgggg gccacataac ccgaaggggt ttcaattgac aaccatagca

      661 ctagctaaga caacgggcac aacacccgca caaactcgca ctgcgcaacc ccgcacaaca

      721 tcgggtctag gtaacactga aatagaagtg aacacctcta aggaaccgca ggtcaatgag

      781 ggttctaagg tcactcgcgc tagggcgtgg cgtaggcaaa acgtcatgta caagatcacc

      841 aatagtaagg ctctggcggg gtgccatagg tggcgcaggg acgaagctgt tgcggtgtcc

      901 tggtcgtcta acggtgcttc gcagtttgag ggtctgcaaa actctcactc tcgctggggg

      961 tcatctctgg ctgaattgga agtcatgggc gaacgccgca ttgagctggc tattgctact

     1021 aagaatcact tggcggcggg tggcgcgctc atgatgtttg tgggcactgt tcgacacaac

     1081 cgctcacagt catttgcgca ggttgaagcg ggtattaaga ctgcgtactc ttcgatggtg

     1141 aaaacatctc agtggaagaa agaacgtgca cggtacgggg tggagcacac ctatagtgac

     1201 tatgaggtca cagactcttg ggcgaacggt tggcacttgc accgcaacat gctgttgttc

     1261 ttggatcgtc cactgtctga cgatgaactc aaggcgtttg aggattccat gttttcccgc

     1321 tggtctgctg gtgtggttaa ggccggtatg gacgcgccac tgcgtgagca cggggtcaaa

     1381 cttgatcagg tgtctacctg gggtggagac gctgcgaaaa tggcaaccta cctcgctaag

     1441 ggcatgtctc aggaactgac tggctccgct actaaaaccg cgtctaaggg gtcgtacacg

     1501 ccgtttcaga tgttggatat gttggccgat caaagcgacg ccggcgagga tatggacgct

     1561 gttttggtgg ctcggtggcg tgagtatgag gttggttcta aaaacctgcg ttcgtcctgg

     1621 tcacgtgggg ctaagcgtgc tttgggcatt gattacatag acgctgatgt acgtcgtgaa

     1681 atggaagaag aactgtacaa gctcgccggt ctggaagcac cggaacgggt cgaatcaacc

     1741 cgcgttgctg ttgctttggt gaagcccgat gattggaaac tgattcagtc tgatttcgcg

     1801 gttaggcagt acgttctcga ttgcgtggat aaggctaagg acgtggccgc tgcgcaacgt

     1861 gtcgctaatg aggtgctggc aagtctgggt gtggattcca ccccgtgcat gatcgttatg

     1921 gatgatgtgg acttggacgc ggttctgcct actcatgggg acgctactaa gcgtgatctg

     1981 aatgcggcgg tgttcgcggg taatgagcag actattcttc gcacccacta aaagcggcat

     2041 aaaccccgtt cgatattttg tgcgatgaat ttatggtcaa tgtcgcgggg gcaaactatg

     2101 atgggtcttg ttgttggcgt cccggagcgt aaaggatcta ggtgaagatc ctttttgata

     2161 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccttgatg

     2221 ataccgctgc cttactgggt gcattagcca gtctgaatga cctgtcacgg gataatccga

     2281 agtggtcaga ctggaaaatc agagggcagg aactgctgaa cagcaaaaag tcagatagca

     2341 ccacatagca gacccgccat aaaacgccct gagaagcccg tgacgggctt ttcttgtatt

     2401 atgggtagtt tccttgcatg aatccataaa aggcgcctgt agtgccattt acccccattc

     2461 actgccagag ccgtgagcgc agcgaactga atgtcacgaa aaagacagcg actcaggtgc

     2521 ctgatggtcg gagacaaaag gaatattcag cgatttgccc gagcttgcga gggtgctact

     2581 taagccttta gggttttaag gtctgttttg tagaggagca aacagcgttt gcgacatcct

     2641 tttgtaatac tgcggaactg actaaagtag tgagttatac acagggctgg gatctattct

     2701 ttttatcttt ttttattctt tctttattct ataaattata accacttgaa tataaacaaa

     2761 aaaaacacac aaaggtctag cggaatttac agagggtcta gcagaattta caagttttcc

     2821 agcaaaggtc tagcagaatt tacagatacc cacaactcaa aggaaaagga ctagtaatta

     2881 tcattgacta gcccatctca attggtatag tgattaaaat cacctagacc aattgagatg

     2941 tatgtctgaa ttagttgttt tcaaagcaaa tgaactagcg attagtcgct atgacttaac

     3001 ggagcatgaa accaagctaa ttttatgctg tgtggcacta ctcaacccca cgattgaaaa

     3061 ccctacaagg aaagaacgga cggtatcgtt cacttataac caatacgctc agatgatgaa

     3121 catcagtagg gaaaatgctt atggtgtatt agctaaagca accagagagc tgatgacgag

     3181 aactgtggaa atcaggaatc ctttggttaa aggctttgag attttccagt ggacaaacta

     3241 tgccaagttc tcaagcgaaa aattagaatt agtttttagt gaagagatat tgccttatct

     3301 tttccagtta aaaaaattca taaaatataa tctggaacat gttaagtctt ttgaaaacaa

     3361 atactctatg aggatttatg agtggttatt aaaagaacta acacaaaaga aaactcacaa

     3421 ggcaaatata gagattagcc ttgatgaatt taagttcatg ttaatgcttg aaaataacta

     3481 ccatgagttt aaaaggctta accaatgggt tttgaaacca ataagtaaag atttaaacac

     3541 ttacagcaat atgaaattgg tggttgataa gcgaggccgc ccgactgata cgttgatttt

     3601 ccaagttgaa ctagatagac aaatggatct cgtaaccgaa cttgagaaca accagataaa

     3661 aatgaatggt gacaaaatac caacaaccat tacatcagat tcctacctac ataacggact

     3721 aagaaaaaca ctacacgatg ctttaactgc aaaaattcag ctcaccagtt ttgaggcaaa

     3781 atttttgagt gacatgcaaa gtaagcatga tctcaatggt tcgttctcat ggctcacgca

     3841 aaaacaacga accacactag agaacatact ggctaaatac ggaaggatct gaggttctta

     3901 tggctcttgt atctatcagt gaagcatcaa gactaacaaa caaaagtaga acaactgttc

     3961 accgggcccg tgggtggcta ggcaagttac agaactgatc ggataaaagc agagttatat

     4021 ctgatgaatt gctattagca gtatcgttat cacagcacca acaaagtagt tcagccacag

     4081 gaaaactttc caactgcgat tagcctgttc acaactggca tctgtaatgt tccaaaatcg

     4141 tgcggcatta aatacgtaag ttagaatcgc aatcccgatg atccacgccg gattaggcaa

     4201 agtagtgact aacacagcag ctagtaaata aagtactact gaaagccgaa tggctccacg

     4261 cgccccaatt acagtggcaa ttgagctcag attagcttcc cggtctgcat taacatcctg

     4321 tactgctcca aggatctgac tggccatgcc ccacaagaaa aaggatccca gtgctatcca

     4381 catcgctgct gaaggagatg ttccagtgat cgttgcaccg attaatgcag gtgaagtgaa

     4441 gtgagtagaa gatgttagag catcgataaa ggggcgttct ttaaaacgca atttcggtgc

     4501 tgaataagca atcactgcta gcactgagag tgtcagccat aaagacgaca tccaggtgcc

     4561 aaatatgaaa agaataacta ggaaaggaat tgttgagata gccgaggccc ataacagtgt

     4621 gctgtgggaa cttttcggta gcacggcccc ctcgacgccg cctttgcggg gattacgcat

     4681 atcagattcg taatcaaaaa catcgttgat accatacatg gcgatgttat acgggataag

     4741 aaaaaatacg atgcctagcc aaaacagcca gtcaatctct cctgcattta ataggtaggc

     4801 cagaccaaag gggtaggcgg tattgatcca gctaatgggg cgagatgaca atagaattag

     4861 tcttattttt tccatcatga ctacggcttt tctggctc