• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒图谱1： png/1-1P121194A4156.png

质粒序列： 点击查看序列

LOCUS       Exported                6075 bp ds-DNA     circular SYN 21-JAN-2018

FEATURES             Location/Qualifiers

     source          1..6075

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        202..581

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        582..785

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     promoter        830..848

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     CDS             935..1591

                     /codon_start=1

                     /product="glutathione S-transferase from Schistosoma 

                     japonicum"

                     /label=GST

                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK

                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY

                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL

                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"

     polyA_signal    1642..1866

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      1912..2340

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2354..2683

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     CDS             2750..3544

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    3718..3839

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(4290..4875)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(5046..5906)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5907..6011)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt

       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct

      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg

      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta

      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg

      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc

      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa

      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca

      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta

      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt

      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg

      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca

      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca

      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc

      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc

      901 cactagtcca gtgtggtgga attctgcaga tatcatgtcc cctatactag gttattggaa

      961 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga

     1021 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg

     1081 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat

     1141 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg

     1201 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat

     1261 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat

     1321 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac

     1381 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg

     1441 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat

     1501 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac

     1561 gtttggtggt ggcgaccatc ctccaaaatg agcggccgct cgagtctaga gggcccgttt

     1621 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct

     1681 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg

     1741 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc

     1801 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct

     1861 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct

     1921 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg

     1981 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg

     2041 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac

     2101 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct

     2161 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt

     2221 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt

     2281 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt

     2341 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag

     2401 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc

     2461 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc

     2521 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg

     2581 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca

     2641 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg

     2701 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca

     2761 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg

     2821 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg

     2881 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc

     2941 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt

     3001 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc

     3061 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca

     3121 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc

     3181 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg

     3241 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct

     3301 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc

     3361 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc

     3421 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta

     3481 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt

     3541 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga

     3601 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac

     3661 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac

     3721 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat

     3781 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat

     3841 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt

     3901 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag

     3961 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg

     4021 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg

     4081 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc

     4141 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc

     4201 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg

     4261 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat

     4321 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag

     4381 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga

     4441 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg

     4501 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt

     4561 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac

     4621 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc

     4681 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt

     4741 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc

     4801 ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga

     4861 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac