• 规格：20μl质粒
• • 质粒宿主：  植物
  • 质粒用途：  蛋白表达
  • 片段类型：  ORF
  • 片段物种：  空载体
  • 原核抗性：  Kan
  • 筛选标记：  Neo/G418
  • 荧光标记：  
  • 启动子:  35S
  • 复制子:  pBBR1
  • 拷贝数:  
  • 感受态:  DH5a
  • 温度:  37度
  • 背景载体:  pYBA-1132
  • 正向引物:  
  • 反向引物:  
  • 诱导方式:
• 质粒简介：             pYBA-1132是一个植物表达质粒。
• 质粒图谱1： png/9-1P51411151Q00.png

 

质粒序列： 点击查看序列

LOCUS       Exported                6566 bp ds-DNA     circular SYN 14-MAY-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..6566

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             267..1061

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /note="Kan"

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     misc_feature    1248..1272

                     /note="LB T-DNA repeat"

                     /note="left border repeat from nopaline C58 T-DNA"

     promoter        1375..1554

                     /note="NOS promoter"

                     /note="nopaline synthase promoter"

     CDS             1599..2393

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /note="Neo"

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     terminator      2394..2646

                     /note="NOS terminator"

                     /note="nopaline synthase terminator and poly(A) signal"

     promoter        2748..3093

                     /note="CaMV 35S promoter"

                     /note="strong constitutive promoter from cauliflower mosaic

                     virus"

     misc_feature    3098..3205

                     /note="MCS"

                     /note="pBluescript multiple cloning site"

     CDS             3206..3922

                     /codon_start=1

                     /product="enhanced GFP"

                     /note="EGFP"

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     misc_feature    4151..4175

                     /note="RB T-DNA repeat"

                     /note="right border repeat from nopaline C58 T-DNA"

     CDS             complement(4394..5056)

                     /codon_start=1

                     /product="replication protein for the broad-host-range 

                     plasmid pBBR1 from Bordetella bronchiseptica"

                     /note="pBBR1 Rep"

                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH

                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV

                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE

                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"

     rep_origin      5057..5826

                     /note="pBBR1 oriV"

                     /note="replication origin of the broad-host-range plasmid 

                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 

                     Rep protein for replication"

     rep_origin      5978..6566

                     /direction=RIGHT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 ctgggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa

       61 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag

      121 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag

      181 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc

      241 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc

      301 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct

      361 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg

      421 acctgtccgg tgccctgaat gaacttcagg acgaggcagc gcggctatcg tggctggcca

      481 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc

      541 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga

      601 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc

      661 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc

      721 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg

      781 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct

      841 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc

      901 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc

      961 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc

     1021 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga

     1081 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt

     1141 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg

     1201 cggggatctc atgctggagt tcttcgccca cccccatggg caaatcgtgg caggatatat

     1261 tgtggtgtaa acacgcgtca cgtggcgcgc cgatcatgag cggagaatta agggagtcac

     1321 gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg

     1381 caacgttgaa ggagccactc agccgcgggt ttctggagtt taatgagcta agcacatacg

     1441 tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agcaaatatt

     1501 tcttgtcaaa aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct

     1561 catattcact ctcaatccaa ataatctgca ccggatctat gattgaacaa gatggattgc

     1621 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga

     1681 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt

     1741 ttgtcaagac cgacctgtcc ggtgccctga atgaacttca ggacgaggca gcgcggctat

     1801 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg

     1861 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg

     1921 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc

     1981 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga

     2041 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag

     2101 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc

     2161 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg

     2221 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata

     2281 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg

     2341 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagatcgtt

     2401 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta

     2461 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt

     2521 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag

     2581 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac

     2641 tagatcggga ttaataggcc tcgtacgacc ggtaacatgg tggagcacga cactctggtc

     2701 tactccaaaa atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa

     2761 caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc

     2821 gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag

     2881 gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg

     2941 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac

     3001 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct

     3061 atataaggaa gttcatttca tttggagagg acaattggag ctccaccgcg gtggcggccg

     3121 ctctagaact agtggatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt

     3181 cgacctcgag ggggggcccg gtaccatggt gagcaagggc gaggagctgt tcaccggggt

     3241 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg

     3301 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg

     3361 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt

     3421 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg

     3481 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga

     3541 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa

     3601 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta

     3661 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat

     3721 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg

     3781 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc

     3841 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct

     3901 cggcatggac gagctgtaca agtcgcgatg actaactagc aaatcaccag tctctctcta

     3961 caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga

     4021 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta

     4081 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt

     4141 ttaaacgcgt tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttgt

     4201 attctgcctc ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc

     4261 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag

     4321 gtatagggcg gcgaggcggc tacagccgat agtctggaac agcgcactta cgggttgctg