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- 规格：20μl质粒
- 复制子：pUC
- 质粒大小：3851bp
- 原核抗性：氨苄青霉素Amp
- 克隆菌株：大肠杆菌DH5α
- 质粒简介： pDrive是一个基因克隆质粒。
- 质粒图谱1： png/3-16102G61543549.png
质粒序列： 点击查看序列LOCUS Exported 3851 bp ds-DNA circular SYN 09-一月-2013
DEFINITION Bacterial vector for UA cloning and expression of a PCR product.
KEYWORDS pDrive
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3851)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported 2016年9月18日 from SnapGene Viewer 2.8.1
http://www.mialingbio.com
COMMENT Linearize at EcoRV to create UA cloning vector.
FEATURES Location/Qualifiers
source 1..3851
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 142..172
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 180..196
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 204..220
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 216..596
/codon_start=1
/gene="lacZ (fragment)"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSSNTTHYRESSVPRMLQTRYVSDPEFVISEFVDKLLEPR
LALDHTCGGPSSRPLYSIVSPKWPHNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRN
SEEARTDRPSQQLRSLNGEWKL"
promoter 239..257
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 266..393
/note="MCS"
/note="multiple cloning site"
promoter complement(399..417)
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(431..447)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(588..1043)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1070..1174
/gene="bla"
/note="AmpR promoter"
CDS 1175..2035
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS 2181..2996
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 3081..3669
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta
241 atacgactca ctatagggaa agctcggtac cacgcatgct gcagacgcgt tacgtatcgg
301 atccagaatt cgtgatatct gaattcgtcg acaagcttct cgagcctagg ctagctctag
361 accacacgtg tgggggcccg agctcgcggc cgctgtattc tatagtgtca cctaaatggc
421 cgcacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
481 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
541 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
601 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
661 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
721 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
781 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
841 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
901 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
961 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
1021 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
1081 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
1141 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
1201 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
1261 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
1321 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
1381 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
1441 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
1501 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
1561 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
1621 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
1681 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
1741 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
1801 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
1861 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
1921 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
1981 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
2041 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
2101 gatctaggtg aagatccttt ttgataatct catgaacaat aaaactgtct gcttacataa
2161 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tctaggccgc
2221 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
2281 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
2341 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
2401 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
2461 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc
2521 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
2581 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
2641 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
2701 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
2761 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
2821 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
2881 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
2941 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaagaat
3001 taattcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
3061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
3121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
3181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
3241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
3301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
3361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
3421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
3481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
3541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
3601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
3661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
3721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
3781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
3841 gaagcggaag a
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