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- 规格：20μl质粒
- 质粒图谱1： png/1-1F92QGTL63.png
质粒序列： 点击查看序列LOCUS Exported 4563 bp ds-DNA circular SYN 26-APR-2017DEFINITION synthetic circular DNAACCESSION .VERSION .KEYWORDS pttq18SOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 4563)AUTHORS .TITLE Direct SubmissionJOURNAL Exported Sep 28, 2017 from MiaoLingPlasmidhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..4563/organism="synthetic DNA construct"/mol_type="other DNA"rep_origin 897..1485/direction=RIGHT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"promoter 1927..1955/label=tac promoter/note="strong E. coli promoter; hybrid between the trp andlac UV5 promoters"protein_bind 1963..1979/label=lac operator/bound_moiety="lac repressor encoded by lacI"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."CDS 1999..2322/codon_start=1/gene="lacZ fragment"/product="LacZ-alpha fragment of beta-galactosidase"/label=lacZ-alpha/translation="MNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRINSLFWRMREDFQPDTD"misc_feature 2001..2057/label=MCS/note="pUC18/19 multiple cloning site"primer_bind complement(2061..2077)/label=M13 fwd/note="common sequencing primer, one of multiple similarvariants"terminator 2478..2564/gene="Escherichia coli rrnB"/label=rrnB T1 terminator/note="transcription terminator T1 from the E. coli rrnBgene"terminator 2656..2683/label=rrnB T2 terminator/note="transcription terminator T2 from the E. coli rrnBgene"promoter 3051..3128/gene="lacI (mutant)"/label=lacIq promoter/note="In the lacIq allele, a single base change in thepromoter boosts expression of the lacI gene about 10-fold."CDS 3129..4211/codon_start=1/gene="lacI"/product="lac repressor"/label=lacI/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"promoter 4324..4428/gene="bla"/label=AmpR promoterCDS 4429..726/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"ORIGIN1 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca61 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg121 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca181 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata241 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag301 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg361 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca421 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta481 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct541 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca601 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag661 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat721 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt781 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa841 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga901 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg961 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc1021 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag1081 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc1141 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg1201 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac1261 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga1321 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt1381 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag1441 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg1501 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta1561 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc1621 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc1681 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gaattaattc tcatgtttga1741 cagcttatca tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc1801 tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca aggcgcactc1861 ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat attctgaaat1921 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat1981 ttcacacagg aaacagcgat gaattcgagc tcggtacccg gggatcctct agagtcgacc2041 tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct2101 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc2161 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc2221 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat aaattccctg2281 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg2341 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc2401 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt2461 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt2521 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt2581 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca2641 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc2701 ttcctgtcgt catatctaca agccatcccc ccacagatac ggtaaactag cctcgttttt2761 gcatcaggaa agcagggaat ttatggtgca ctctcagtac aatctgctct gatgccgcat2821 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc2881 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt2941 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat3001 aggttaatgt catgataata atggtttctt agacgtgagg ttctgtaccc gacaccatcg3061 aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg3121 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc3181 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag3241 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg3301 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc3361 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga3421 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac3481 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag3541 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca3601 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg&
