• 规格：20μl质粒
• 质粒图谱1： png/1-1F92Q15K2S6.png

质粒序列： 点击查看序列

LOCUS       Exported                5818 bp ds-DNA     circular SYN 28-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5818)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 28, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..5818

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        473..1651

                     /label=EF-1-alpha promoter

                     /note="strong constitutive promoter for human elongation 

                     factor EF-1-alpha"

     promoter        1668..1686

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     misc_feature    1713..1792

                     /label=MCS

     CDS             1805..1846

                     /codon_start=1

                     /product="epitope tag from simian virus 5"

                     /label=V5 tag

                     /translation="GKPIPNPLLGLDST"

     CDS             1856..1873

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /label=6xHis

                     /translation="HHHHHH"

     polyA_signal    1902..2126

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      2172..2600

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2614..2943

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      2794..2929

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     promoter        2991..3038

                     /label=EM7 promoter

                     /note="synthetic bacterial promoter "

     CDS             3057..3455

                     /codon_start=1

                     /gene="Aspergillus terreus BSD"

                     /product="blasticidin S deaminase"

                     /label=Blast

                     /note="confers resistance to blasticidin"

                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG

                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI

                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"

     polyA_signal    3613..3734

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     protein_bind    3807..3823

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        complement(3831..3861)

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    3876..3897

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence 

                     of cAMP."

     rep_origin      complement(4185..4773)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(4944..5804)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5805..91)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta

       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg

      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga

      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg

      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct

      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca

      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga

      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg

      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt

      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt

      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc

      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg

      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac

      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc

      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg

      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa

      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag

     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg

     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc

     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc

     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg

     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg

     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc

     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc

     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc

     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt

     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca

     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac

     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag

     1741 tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggccctt

     1801 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca

     1861 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca

     1921 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac

     1981 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat

     2041 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca

     2101 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag

     2161 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg

     2221 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc

     2281 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg

     2341 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc

     2401 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt

     2461 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc

     2521 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta

     2581 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc

     2641 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg

     2701 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag

     2761 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc

     2821 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc

     2881 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc

     2941 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt

     3001 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg

     3061 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca

     3121 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca

     3181 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc

     3241 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg

     3301 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc