• 规格：10μl质粒
• 质粒图谱1： png/1-1F92Q11305149.png

质粒序列： 点击查看序列

LOCUS       Exported                6624 bp ds-DNA     circular SYN 28-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pRH2521

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 6624)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 28, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..6624

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    63..182

                     /label=Pimyc promoter

     terminator      197..224

                     /label=rrnB T2 terminator

                     /note="transcription terminator T2 from the E. coli rrnB 

                     gene"

     misc_feature    244..423

                     /label=Pmyc1 promoter

     protein_bind    424..441

                     /gene="tetO"

                     /label=tet operator

                     /bound_moiety="tetracycline repressor TetR"

                     /note="bacterial operator O2 for the tetR and tetA genes"

     misc_RNA        503..578

                     /label=gRNA scaffold

                     /note="guide RNA scaffold for the Streptococcus pyogenes 

                     CRISPR/Cas9 system"

     CDS             complement(738..1361)

                     /codon_start=1

                     /label=TetR

                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV

                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR

                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT

                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"

     misc_feature    complement(1389..1508)

                     /label=Pimyc promoter

     terminator      1588..1615

                     /label=rrnB T2 terminator

                     /note="transcription terminator T2 from the E. coli rrnB 

                     gene"

     rep_origin      complement(1876..2464)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(2843..4006)

                     /codon_start=1

                     /label=Hyg

                     /translation="MPGPFTPAAAERPPPGPHRRRPARSGLAAAGFGAGPGLPSAGGAP

                     GGRPPGAGGGAGRPGASGAGGGVRRPPEELRQFLRIGEAGEIQRGLLEHLEVVQEGEGE

                     QFAGEVLGPLPLRPVEQRGQDLAVAPEGVEMQLHQAVAGVSRIDVGEVDDPGDLGRGQV

                     HEDVGPVQVAVDEPGFAAGQQRVHVRQPVLQAVQQPGREVAPPAVVLDGRRAAFPQQFR

                     EDLGMGGEHGVPGQRHPVQPAEHPAEFAGQGEQRVPVGRAVHRGPPGGAGHPAHHQVGP

                     RPGSGAGPQLAAAEEAGHRHRGVRQDRVRLRLRREALRTAPVLAEQLDHRAGLADQYGV

                     GALAGHPQHRRHRQPELLQGSAGQRLPEFLVVPQARVGIIRINTVEK"

     misc_feature    complement(4090..6616)

                     /label=oriM

ORIGIN

        1 aacgttctcg gctcgatgat ccccgggggc cccccctcga ggtcgacggt atcgataata

       61 ttggatcgtc gcaccgggtt aagcctggag tgcggtggtg cctggtcggc attttcgcag

      121 tcgagggctc tcgtgtagcc tgggcgagtt gccgacgcag gcgaccctcc tgccacggat

      181 ccgtgattag ctaagcagaa ggctatcctg acggatggcc tttttgcgtt taatactgtt

      241 taaactctag aaatattgga tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg

      301 cgacgtgatg atcggccgca tccccacggt gctgcgcagt gagctctacg ccatcccggc

      361 gttgatctgt gcgttcgcac gcacaggccc ggtgtgagaa gggtctctga cgagcgggag

      421 aactccctat cagtgataga gtttgtcctc cctatcagtg atagataggc tatgggagtg

      481 tcttcgagac agaggaagac atgttttaga gctagaaata gcaagttaaa ataaggctag

      541 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttatcgat ttaattaata

      601 tgcatcaatt gatttaaata ctagtgttaa ctatttaatt ggggacccta gaggtcccct

      661 tttttatttt aaaaattttt tcaaggggac ctctagggtc cccagctggc tagcgagtca

      721 tgaggtcacc atggtcatta ggagccgctc tcgcacttca gctgcttctc caagccgcag

      781 atgatcagct ccaggccgaa caagaaggcc ggctcggcgc cctggtggtc gaacagctcg

      841 atggcctggc gcagcaacgg cggcatgctg tcggtggtcg gggtctcgcg ctcctccttg

      901 gcgacctggt gctcctggtc ctccaacacg cagcccaggg tgaagtggcc cacggccgac

      961 agggcgtaca acgcgttctc cagcgagaag ccctgctggc acaggaaggc caactggttc

     1021 tccagggtct cgtactgctt ctcggtcggc cgggtgccca ggtgcacctt ggcgccgtcg

     1081 cggtggctca gcaaggcgca gcggaagctc ttggcgttgt tgcgcaggaa gtcctgccag

     1141 ctctcgccct ccagcgggca gaagtgggtg tggtggcggt ccaacatctc gatggccagg

     1201 gcgtcgagca gggcccgctt gttcttgacg tgccagtaca gggtcggctg ctcgacgccc

     1261 agcttctggg ccagcttgcg ggtggtcagg ccctcgatgc cgacctcgtt cagcagctcc

     1321 agggcggagt tgatgacctt ggacttgtcc aggcgggaca ttgcgaagtg attcctcctg

     1381 atggtaccgg atccgtggca ggagggtcgc ctgcgtcggc aactcgccca ggctacacga

     1441 gagccctcga ctgcgaaaat gccgaccagg caccaccgca ctccaggctt aacccggtgc

     1501 gacgatccaa tattacgaga tctgatatcc tcgaggcatg ccacgtgcct gcaggtttaa

     1561 acgcggccgc aaacagtatt aaacgcaaaa aggccatccg tcaggatggc cttctgctta

     1621 gctaatcaac tagttctagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt

     1681 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct

     1741 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga

     1801 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc

     1861 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg

     1921 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg

     1981 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt

     2041 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt

     2101 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg

     2161 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact

     2221 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt

     2281 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct

     2341 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac

     2401 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc

     2461 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg

     2521 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta

     2581 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca

     2641 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc

     2701 ctgactcccc gacgtggccg accagcccgt catcgtcaac gcctgatccg cggtgcggac

     2761 aggccgtgtc gcgaccggcc gtgcggaatt aagccggccc gtaccctgtg aatagaggtc

     2821 cgctgtgaca caagaatccc tgttacttct cgaccgtatt gattcggatg attcctacgc

     2881 gagcctgcgg aacgaccagg aattctggga gccgctggcc cgccgagccc tggaggagct

     2941 cgggctgccg gtgccgccgg tgctgcgggt gcccggcgag agcaccaacc ccgtactggt

     3001 cggcgagccc ggcccggtga tcaagctgtt cggcgagcac tggtgcggtc cggagagcct

     3061 cgcgtcggag tcggaggcgt acgcggtcct ggcggacgcc ccggtgccgg tgccccgcct

     3121 cctcggccgc ggcgagctgc ggcccggcac cggagcctgg ccgtggccct acctggtgat

     3181 gagccggatg accggcacca cctggcggtc cgcgatggac ggcacgaccg accggaacgc

     3241 gctgctcgcc ctggcccgcg aactcggccg ggtgctcggc cggctgcaca gggtgccgct

     3301 gaccgggaac accgtgctca ccacccattc cgagatcttc ccggaactgc tgcgggaacg

     3361 ccgcgcggcg accgtcgagg accaccgcgg gtggggctac ctctcgcccc ggctgctgga

     3421 ccgcctggag gactggctgc cggacgtgga cacgctgctg gccggccgcg aaccccggtt

     3481 cgtccacggc gacctgcacg ggaccaacat cttcgtggac ctggccgcga ccgaggtcac

     3541 cgggatcgtc gacttcaccg acgtctatgc gggagactcc cgctacagcc tggtgcaact

     3601 gcatctcaac gccttccggg gcgaccgcga gatcctggcc gcgctgctcg acggggcgca

     3661 gtggaagcgg accgaggact tcgcccgcga actgctcgcc ttcaccttcc tgcacgactt

     3721 cgaggtgttc gaggagaccc cgctggatct ctccggcttc accgatccgg aggaactggc

     3781 gcagttcctc tgggggccgc cggacaccgc ccccggcgcc tgacgccccg ggccgcccgg

     3841 cgccgccccc ggcccccggc ggccggccgc ccggagcccc gcccgcgctc gggagccccg

     3901 ggcccgcgcc gaagcccgct gctgcgagcc cggagcgggc cggccgacgg cggtgcgggc

     3961 ccggcggcgg acgctcagca gcggcgggcg tgaaaggccc tggcatcctc gatcatctcc

     4021 tccagggtgg tcggccgagc ttccatccca gctcggcaag gatcaacgag gacagtcgca

     4081 cgacgaagtt cttctggatc gcgcccgtgc tggaagcact caacctcgaa gcgtgtggtt

     4141 gcggagccat ctagcaacca cacgaaacat gcgcaacgaa ccgcgcaacg aacaacgcct

     4201 agaactggcc ctagatgagc tgactcgtat cgttggtaaa cctagtttga ccagcatgtt

     4261 ttaactacgt tcggtgagct gtcaacgggg cctgtaacgg cacaacgaac cgtgcaacga

     4321 gagtggccac ggatgccacc acaggcacta caacggagtt cgccacgtac atcaccacaa

     4381 ccaccgattc tggcggtgag ctccccgata ttcagcggaa atggcttggt atcgaccaag

     4441 attcgtagaa ccccgtctcg tctggctggt attcaaaacg ggcgcaacga aacacgcaac

     4501 gagacaggca tggcccaaac cagaaaacta gcgtctacca ggacttttac ctgtccgacc

     4561 cgttgcaacg gaacccccca cggaaccccc gcgacacccg ctccccaatt gcgttagaac

     4621 agcggtggat tgtcggcttc gttgtgggcc ttttgagccg cttcctgttc tgccgcacgc

     4681 tctttcctcg cccgatagcc gagtcgctta acggtgtcca gatgcagccc gaaatgtttg

     4741 gccgtttgcg gccaagagtg gccctcgtcg tcgtgatagg cgcggatgcg ttcgcggcgt

     4801 gcagcctgct cggcgagcca ctcgctgcgt tcctgcgcca cgagccggac gacgtggcgt

     4861 tcggatagtc cggtgattcg agcgccttcg gcggcggtca cgcgccgctt tttgcggaca

     4921 gtcggctgcc ggttgtagcc gtcgctgtag ccgtcgctca tagcaatgcc tccatggctg

     4981 acgcggactt tgcgcgccgc gcaactgtgc tcgccgccgt gcgcgctgct gcgcccttcc

     5041 gcgagatggc cgactggcgc gcactgagtg tggcctcgta gaccacgatc ccgtccgccc

     5101 aaatgcgcga cttggttgtg atccaacgcc aaatgctgtt ggcgatggcg cggacctcgc

     5161 tgtccggtag cggtccggga cacacgtcgt tgcacgggaa ttcggcgttt cgcgcgtggc

     5221 actcggcata gatcgcgcgg ccgagtccgt ccacgttccg ggtcggcagg tagatccgca

     5281 tgagggcggg acgataggcc cacaacctga cggaatcgaa cagtgcgcaa ttccgcccta

     5341 gcggcgtcgg agccgctttg tacgtggtct gctgacgcca gcgcggcggt ggcatgttcg

     5401 cgccgagctc ggcctcgatg tggctgagtg tgtagagatc tgagtggagc cattccgttt

     5461 cccaggcgat gtggccgggg tttttggtca tgaggcctga gtaactgcgg tcgccgtcga

     5521 cggcgcgccg aaggccttcg gcgcacgccg ccatgtatgc gagcggctta cgccgcgcgt

     5581 attcggtgcg tggaacaggg gcgttgagtg cccacactgc gtgtgcgtgg ccgttggcgc

     5641 gattgcccac gatcgcgttg ggcagcggat gggacccccg ggcgctgagc gctcggagcg