• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：红色荧光蛋白mCherry
• 克隆菌株：大肠杆菌DH5α
• 质粒图谱1： png/1-1P112103432Y1.png

质粒序列： 点击查看序列

LOCUS       Exported                3562 bp ds-DNA     circular SYN 12-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..3562

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        20..38

                     /note="T7 promoter"

                     /note="promoter for bacteriophage T7 RNA polymerase"

     misc_feature    70..92

                     /note="RBS"

     CDS             112..129

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /note="6xHis"

                     /translation="HHHHHH"

     CDS             133..165

                     /codon_start=1

                     /product="leader peptide from bacteriophage T7 gene 10"

                     /note="T7 tag (gene 10 leader)"

                     /note="promotes efficient translation in E. coli"

                     /translation="MASMTGGQQMG"

     CDS             169..192

                     /codon_start=1

                     /product="Xpress(TM) epitope tag, including an enterokinase

                     recognition and cleavage site"

                     /note="Xpress(TM) tag"

                     /translation="DLYDDDDK"

     misc_feature    199..909

                     /note="mCherry"

     terminator      977..1024

                     /note="T7 terminator"

                     /note="transcription terminator for bacteriophage T7 RNA 

                     polymerase"

     rep_origin      1121..1576

                     /direction=RIGHT

                     /note="f1 ori"

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        1602..1706

                     /gene="bla"

                     /note="AmpR promoter"

     CDS             1707..2567

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="AmpR"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      2738..3326

                     /direction=RIGHT

                     /note="ori"

                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct

       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat

      121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac

      181 gatgacgata aggatccgat ggtgagcaag ggcgaggagg ataacatggc catcatcaag

      241 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc

      301 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc

      361 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc

      421 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag

      481 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag

      541 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc

      601 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg

      661 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac

      721 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg

      781 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc

      841 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg

      901 tacaagtaag cttgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc

      961 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt

     1021 tttgctgaaa ggaggaacta tatccggatc tggcgtaata gcgaagaggc ccgcaccgat

     1081 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca

     1141 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta

     1201 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt

     1261 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac

     1321 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt

     1381 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga

     1441 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg

     1501 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata

     1561 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt

     1621 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc

     1681 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc

     1741 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa

     1801 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg

     1861 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag

     1921 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc

     1981 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta

     2041 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg

     2101 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca

     2161 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac

     2221 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat

     2281 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg

     2341 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata

     2401 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta

     2461 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa

     2521 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag

     2581 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg

     2641 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact

     2701 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg

     2761 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc

     2821 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata

     2881 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta

     2941 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc

     3001 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg

     3061 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac

     3121 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg

     3181 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt

     3241 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct

     3301 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg

     3361 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata

     3421 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca

     3481 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc

     3541 gttggccgat tcattaatgc ag

//