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- 规格：20μl质粒
- 启动子：T7/lac promoter
- 复制子：ColE1 ori
- 终止子：T7 terminator
- 质粒分类：大肠杆菌载体；PET系列表达质粒
- 质粒大小：5675bp
- 质粒标签：N-T7
- 原核抗性：氨苄青霉素Amp
- 克隆菌株：大肠杆菌DH5α
- 表达宿主：大肠杆菌BL21(DE3)
- 诱导方式：IPTG或乳糖及其类似物
- 5测序引物：T7:TAATACGACTCACTATAGGG
- 3测序引物：T7-ter:TGCTAGTTATTGCTCAGCGG
- 质粒简介： pET-11a-d 载体具有N端T7标签和 BamH I 多克隆克隆位点。这些载体是很多pET家族载体的前身。pET-21a-d( )系列载体分别对应于 pET-11a-d 载体，但是包含有几个额外的特征序列。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
- 质粒图谱1： png/3-161011161634300.png
质粒序列： 点击查看序列LOCUS Exported 5675 bp ds-DNA circular SYN 11-十月-2016
DEFINITION synthetic circular DNA
KEYWORDS pET11c.gb
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5675)
TITLE Direct Submission
http://www.fenghbio.cn
FEATURES Location/Qualifiers
source 1..5675
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 10..38
/note="tet promoter"
/note="E. coli promoter for tetracycline efflux protein
gene"
terminator 213..260
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(326..358)
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
RBS 366..388
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
protein_bind 403..427
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(428..446)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 755..832
/gene="lacI"
/note="lacI promoter"
CDS 833..1915
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
CDS 3227..3418
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
misc_feature 3520..3662
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(3848..4436)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4607..5467)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5468..5572)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
301 cgggctttgt tagcagccgg atccgaccca tttgctgtcc accagtcatg ctagccatat
361 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
421 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc
481 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
541 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
601 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
661 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
721 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt
781 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
841 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
901 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
961 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
1021 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
1081 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
1141 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
1201 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
1261 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
1321 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
1381 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
1441 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
1501 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
1561 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
1621 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
1681 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
1741 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
1801 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
1861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
1921 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga
1981 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
2041 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
2101 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
2161 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
2221 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
2281 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
2341 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
2401 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg
2461 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
2521 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
2581 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc
2641 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat
2701 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc
2761 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
2821 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
2881 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
2941 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
3001 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
3061 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
3121 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
3181 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
3241 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
3301 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
3361 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
3541 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
3601 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
3661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
3721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
3781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
4021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
4261 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
4501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
4561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
4621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
4681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
4741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
4801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
4861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
4921 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
4981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
5041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
5101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
5161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
5221 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
5281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
5341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
5401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
5461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
5521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
5581 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
5641 ataggcgtat cacgaggccc tttcgtcttc aagaa
//
