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- 规格：20μl质粒
- 启动子：Ac5 promoter
- 复制子：pUC ori
- 终止子：SV40 poly(A) signal
- 质粒分类：昆虫系列质粒；昆虫游离质粒；昆虫胞内质粒
- 质粒大小：6118bp
- 质粒标签：N-EGFP，C-V5，C-6×His
- 原核抗性：氨苄青霉素Amp
- 筛选标记：无
- 克隆菌株：DH5α等大肠杆菌
- 表达宿主：果蝇细胞系S2
- 诱导方式：无需诱导，组成型表达
- 5测序引物：Ac5-F(ACACAAAGCCGCTCCATCAG)
- 3测序引物：BGH-R(TAGAAGGCACAGTCGAGG)
- 备注：非病毒
- 质粒简介： pAC5.1b-EGFP-2质粒是一个昆虫细胞组成型表达载体，Ac5启动子驱动EGFP标签、目的基因、Myc标签和6×HIS标签一起融合表达，可以与筛选载体pCoHygro或者pCoBlast共转染，建立稳表达细胞系。本质粒由武汉淼灵生物科技有限公司共享至淼灵质粒平台。 pAC5.1b-EGFP② is a 6kb expression vector designed for use with the Drosophila Constitutive Expression System available from Invitrogen. Upon transfection, the vector allows transient expression of your protein of interest in Drosophila cells. When cotransfected with the selection vector, pCoHygro or pCoBlast, pAC5.1b-EGFP allows selection of stable cell lines exhibiting constitutive expression of the protein of interest. • The Drosophila actin 5C (Ac5) promoter for high-level, constitutive expression of the gene of interest in S2 cells (Chung and Keller, 1990).
• Multiple cloning site to facilitate cloning the gene of interest.
• C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for detection and purification of your protein of interest (if desired).
• Three reading frames to facilitate in-frame cloning with the C-terminal peptide.
• N-terminal EGFP tag for better detection.
• Ampicillin resistance gene for selection of transformants in E. coli.- 质粒图谱1： png/1-1FR50R503K9.png
质粒序列： 点击查看序列
