• 规格：20μl质粒
• 复制子：pUC ori,f1 ori
• 终止子：SV40 poly(A) signal
• 质粒分类：哺乳细胞，信号通路报告载体
• 质粒大小：5064bp
• 原核抗性：Amp
• 克隆菌株：DH5α
• 表达宿主：哺乳细胞
• 诱导方式：无须诱导，瞬时表达
• 5测序引物：RVP3:CTAGCAAAATAGGCTGTCCC
• 3测序引物：根据序列设计引物
• 质粒简介：      The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.
• 质粒图谱1： png/1-1F926154J2B0.png

质粒序列： 点击查看序列

LOCUS       Exported File           5064 bp ds-DNA    circular SYN 23-3-2015

DEFINITION  .

ACCESSION   .

VERSION     .

KEYWORDS    Untitled

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5064)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported 2015-3-23 from MiaoLingplasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..5064

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             88..1740

                     /codon_start=1

                     /gene="luc+"

                     /product="firefly luciferase"

                     /EC_number="

                     "

                     /note="luciferase"

                     /note="enhanced?luc+ version of the luciferase gene"

                     /protein_id="

                     "

                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA

                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP

                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS

                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA

                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK

                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY

                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS

                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL

                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV

                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"

     polyA_signal    1781..1902

                     /note="SV40 poly(A) signal"

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(2567..3155)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3326..4186)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="AmpR"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(4187..4291)

                     /gene="bla"

                     /note="AmpR promoter"

     rep_origin      4318..4773

                     /direction=RIGHT

                     /note="f1 ori"

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     polyA_signal    4904..4952

                     /note="synthetic polyadenylation signal"

     misc_feature    4966..5057

                     /note="pause site"

                     /note="RNA polymerase II transcriptional pause signal from 

                     the human?alpha-2 globin gene"

ORIGIN

        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg

       61 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg

      121 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag

      181 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc

      241 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg

      301 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg

      361 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa

      421 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag

      481 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc

      541 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat

      601 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca

      661 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct

      721 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc

      781 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact

      841 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag

      901 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta

      961 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa

     1021 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc

     1081 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt

     1141 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg

     1201 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt

     1261 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg

     1321 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac

     1381 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc

     1441 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt

     1501 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag

     1561 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag

     1621 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac

     1681 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa

     1741 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt

     1801 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct

     1861 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt

     1921 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc

     1981 tacaaatgtg gtaaaatcga taaggatctg aacgatggag cggagaatgg gcggaactgg

     2041 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga

     2101 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg

     2161 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc

     2221 cacaccctaa ctgacacaca ttccacagcg gatccgtcga ccgatgccct tgagagcctt

     2281 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac

     2341 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc

     2401 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg

     2461 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag

     2521 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc

     2581 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag

     2641 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga

     2701 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc

     2761 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg

     2821 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt

     2881 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca

     2941 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca

     3001 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag

     3061 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca

     3121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg

     3181 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa

     3241 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta

     3301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag

     3361 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga

     3421 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac

     3481 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc

     3541 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta

     3601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac

     3661 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat

     3721 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa

     3781 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg

     3841 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag

     3901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc

     3961 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct

     4021 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat

     4081 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg

     4141 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc

     4201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta

     4261 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg

     4321 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta

     4381 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt

     4441 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg

     4501 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat

     4561 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac

     4621 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag

     4681 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg

     4741 cgaattttaa caaaatatta acgcttacaa tttgccattc gccattcagg ctgcgcaact

     4801 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat

     4861 aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt

     4921 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca

     4981 tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg

     5041 tgccagaaca tttctctatc gata