• 规格：20μl质粒
• 质粒图谱1： png/1-1F926152T9524.png

质粒序列： 点击查看序列

LOCUS       Exported                4732 bp ds-DNA     circular SYN 26-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4732)

  AUTHORS   admin

  TITLE     Direct Submission

  JOURNAL   Exported 2015-10-18   from MiaoLingPlasmid 

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..4732

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        61..364

                     /label=CMV enhancer

                     /note="CMV enhancer;human cytomegalovirus immediate early 

                     enhancer"

     promoter        365..568

                     /label=CMV promoter

                     /note="CMV promoter;human cytomegalovirus (CMV) immediate 

                     early promoter"

     misc_feature    591..671

                     /label=MCS

                     /note="MCS;multiple cloning site"

     CDS             679..1398

                     /codon_start=1

                     /product="yellow fluorescent protein with a large Stokes 

                     shift, excitable with violet light (Ai et al., 2008)"

                     /label=mAmetrine

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL

                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD

                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK

                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL

                     EFVTAAGITPGMDELYK"

     polyA_signal    1520..1641

                     /label=SV40 poly(A) signal

                     /note="SV40 poly(A) signal;SV40 polyadenylation signal"

     rep_origin      complement(1648..2103)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 ori;f1 bacteriophage origin of replication; arrow

                     indicates direction of (+) strand synthesis"

     promoter        2130..2234

                     /gene="bla"

                     /label=AmpR promoter

                     /note="AmpR promoter"

     promoter        2236..2593

                     /label=SV40 promoter

                     /note="SV40 promoter;SV40 enhancer and early promoter"

     rep_origin      2444..2579

                     /label=SV40 ori

                     /note="SV40 ori;SV40 origin of replication"

     CDS             2628..3422

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="NeoR/KanR;confers resistance to neomycin, kanamycin,

                     and G418 (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    3654..3701

                     /label=HSV TK poly(A) signal

                     /note="HSV TK poly(A) signal;herpesvirus thymidine kinase 

                     polyadenylation signal"

     rep_origin      4030..4618

                     /direction=RIGHT

                     /label=ori

                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

ORIGIN

        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg

      661 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc

      721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc

      781 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcacctc cggcaagctg

      841 cccgtgccct ggcccaccct cgtgaccacc ctgtcttacg gcgtgcagtg cttcgcccgc

      901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc

      961 caggagcgca ccatctcctt caaggacgac ggcagctaca ggacccgcgc cgaggtgaag

     1021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac

     1081 ggcaacatcc tggggcacaa gctggagtac aacatgaacg tgtgggacgc gtatatcacg

     1141 gccgacaagc agaagaacgg catcaaagcg aacttcaaga tcgagcacaa cgtcgaggac

     1201 ggcggcgtgc agctcgccga cgcgtaccag cagaacaccc ccatcggcga cggctccgtg

     1261 ctgctgcctg acaaccacta cctgagcttc cagagcaagc tgttcaaaga ccccaacgag

     1321 cagcgcgatc acatggtcct gctggagttc gttaccgccg ccgggatcac tcccggcatg

     1381 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatttgt

     1441 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat

     1501 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa

     1561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc

     1621 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg

     1681 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc

     1741 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga

     1801 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg

     1861 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag

     1921 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga

     1981 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg

     2041 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg

     2101 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa

     2161 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt

     2221 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg

     2281 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc

     2341 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca

     2401 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc

     2461 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc

     2521 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct

     2581 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag

     2641 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg

     2701 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc

     2761 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag

     2821 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca

     2881 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat

     2941 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata

     3001 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac

     3061 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc

     3121 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg

     3181 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg

     3241 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta

     3301 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg

     3361 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct

     3421 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga

     3481 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc

     3541 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg

     3601 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat

     3661 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc

     3721 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt

     3781 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa

     3841 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt

     3901 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac

     3961 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa

     4021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc

     4081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt

     4141 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg

     4201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc

     4261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt

     4321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga

     4381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct

     4441 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg

     4501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca

     4561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa

     4621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt

     4681 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at

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