• 规格：20μl质粒
• 质粒图谱1： png/1-1F926151113163.png

质粒序列： 点击查看序列

LOCUS       Exported                4960 bp ds-DNA     circular SYN 24-AUG-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4960)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 26, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..4960

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        106..409

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        410..613

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     misc_feature    636..716

                     /label=MCS

                     /note="multiple cloning site"

     CDS             724..1671

                     /codon_start=1

                     /product="bright monomeric near-infrared fluorescent 

                     protein engineered from a bacterial phytochrome, with an 

                     emission maximum of 670 nm (Shcherbakova et al., 2016)"

                     /label=miRFP670

                     /label=ORF frame 1

                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH

                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE

                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG

                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT

                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV

                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"

     polyA_signal    1793..1914

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(1921..2376)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2403..2507

                     /gene="bla"

                     /label=AmpR promoter

     promoter        2509..2866

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      2717..2852

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             2901..3695

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    3927..3974

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      4303..4891

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta

       61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac

      121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac

      181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt

      241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat

      301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga

      361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt

      421 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca

      481 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg

      541 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta

      601 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc

      661 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc

      721 accatggtag caggtcatgc ctctggcagc cccgcattcg ggaccgcctc tcattcgaat

      781 tgcgaacatg aagagatcca cctcgccggc tcgatccagc cgcatggcgc gcttctggtc

      841 gtcagcgaac atgatcatcg cgtcatccag gccagcgcca acgccgcgga atttctgaat

      901 ctcggaagcg tactcggcgt tccgctcgcc gagatcgacg gcgatctgtt gatcaagatc

      961 ctgccgcatc tcgatcccac cgccgaaggc atgccggtcg cggtgcgctg ccggatcggc

     1021 aatccctcta cggagtactg cggtctgatg catcggcctc cggaaggcgg gctgatcatc

     1081 gaactcgaac gtgccggccc gtcgatcgat ctgtcaggca cgctggcgcc ggcgctggag

     1141 cggatccgca cggcgggttc actgcgcgcg ctgtgcgatg acaccgtgct gctgtttcag

     1201 cagtgcaccg gctacgaccg ggtgatggtg tatcgtttcg atgagcaagg ccacggcctg

     1261 gtattctccg agtgccatgt gcctgggctc gaatcctatt tcggcaaccg ctatccgtcg

     1321 tcgactgtcc cgcagatggc gcggcagctg tacgtgcggc agcgcgtccg cgtgctggtc

     1381 gacgtcacct atcagccggt gccgctggag ccgcggctgt cgccgctgac cgggcgcgat

     1441 ctcgacatgt cgggctgctt cctgcgctcg atgtcgccgt gccatctgca gttcctgaag

     1501 gacatgggcg tgcgcgccac cctggcggtg tcgctggtgg tcggcggcaa gctgtggggc

     1561 ctggttgtct gtcaccatta tctgccgcgc ttcatccgtt tcgagctgcg ggcgatctgc

     1621 aaacggctcg ccgaaaggat cgcgacgcgg atcaccgcgc ttgagagcta agcggccgcg

     1681 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct

     1741 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt

     1801 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc

     1861 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt

     1921 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat

     1981 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga

     2041 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca

     2101 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct

     2161 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc

     2221 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag

     2281 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca

     2341 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg

     2401 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga

     2461 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa

     2521 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg

     2581 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg

     2641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc

     2701 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca

     2761 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt

     2821 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga

     2881 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg

     2941 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg

     3001 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt

     3061 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg

     3121 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat

     3181 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat

     3241 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg

     3301 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg

     3361 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc

     3421 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc

     3481 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg

     3541 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg

     3601 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca

     3661 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac

     3721 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga

     3781 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga

     3841 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac

     3901 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt

     3961 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc

     4021 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc

     4081 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc

     4141 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat

     4201 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt

     4261 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct

     4321 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc

     4381 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc

     4441 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc

     4501 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc

     4561 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg

     4621 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata

     4681 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta