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- 规格：20μl质粒
- 启动子：HpaII promoter
- 复制子：ColE1 Ori，F1 Ori
- 质粒分类：枯草杆菌质粒；枯草游离质粒；枯草胞内质粒
- 质粒大小：7202bp
- 原核抗性：氨苄青霉素Amp
- 克隆菌株：DH5α等大肠杆菌
- 质粒简介： pMA5质粒是一个枯草芽孢杆菌表达载体，HpaII promoter驱动目的基因的表达。
- 质粒图谱1： png/1-1F9151IT31O.png
质粒序列： 点击查看序列LOCUS Exported 7202 bp ds-DNA circular SYN 08-九月-2017KEYWORDS pMA5SOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 7202)TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..7202/organism="synthetic DNA construct"/mol_type="other DNA"rep_origin 429..884/direction=RIGHT/note="f1 ori"/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 1006..1110/gene="bla"/note="AmpR promoter"CDS 1111..1971/codon_start=1/note="Amp"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"rep_origin 2142..2733/direction=RIGHT/note="ori"/note="high-copy-number colE1/pMB1/pBR322/pUC origin ofreplication"misc_feature 5317..6078/note="Kan"misc_feature 6934..3/note="HpaII promoter"ORIGIN1 catatgagtt atgcagtttg tagaatgcaa aaagtgaaat cagggggatc ctctagagtc61 gagctcaagc tagcttggta cgtaccagat ctgagatcac gcgttctaga ggtcgaaatt121 cacctcgaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga gccttttttt181 ttggagattt tcaacgtgaa aaaattatta ttcgcaattc caagctaatt cacctcgaaa241 gcaagctgat aaaccgatac aattaaaggc tccttttgga gccttttttt ttggagattt301 tcaacgtgaa aaaattatta ttcgcaattc caagctctgc ctcgcgcgtt tcggtgatga361 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga421 tgcagatcac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag481 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt541 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt601 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg661 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt721 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt781 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca841 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttgatctg cgctcggtcg901 ttcggctgcg gcgagcggta tcagctcact caaagcggta atacggttat cccagaatcc961 ggggataacg caggaaagac aggtggcact tttcggggaa atgtgcgcgg aacccctatt1021 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa1081 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt1141 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa1201 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac1261 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt1321 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt1381 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat1441 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac1501 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg1561 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc1621 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa1681 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag1741 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct1801 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat1861 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa1921 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac1981 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc2041 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc2101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg2161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg2221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca2281 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg2341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg2401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga2461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga agtactgaga2521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg2581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac2641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg2701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg2761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct2821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc2881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc2941 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg3001 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta3061 cactttatgc ttccggcata ttctcaataa accctttagg gaaataggcc aggttttcac3121 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt3181 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa3241 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat3301 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta3361 cggtctttaa aaaggccgta atatccagct aaacggtctg gttataggta cattgagcaa3421 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat3481 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa3541 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc3601 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac3661 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg aagacgaaag3721 ggcatcgcgc gcggggaatt cccgggagag ctcgatatcg catgcggtac ctctagaaga3781 agcttggaga caaggtaaag gataaaacag cacaattcca agaaaaacac gatttagaac3841 ctaaaaagaa cgaatttgaa ctaactcata accgagaggt aaaaaaagaa cgaagtcgag3901 atcagggaat gagtttataa aataaaaaaa gcacctgaaa aggtgtcttt ttttgatggt3961 tttgaacttg ttctttctta tcttgataca tatagaaata acgtcatttt tattttagtt4021 gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta aagtattgaa aacccttaaa4081 attggttgca cagaaaaacc ccatctgtta aagttataag tgactaaaca aataactaaa4141 tagatggggg tttcttttaa tattatgtgt cctaatagta gcatttattc agatgaaaaa4201 tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga gaccatggag agaaaagaaa4261 atcgctaatg ttgattactt tgaacttctg catattcttg aatttaaaaa ggctgaaaga4321 gtaaaagatt gtgctgaaat attagagtat aaacaaaatc gtgaaacagg cgaaagaaag4381 ttgtatcgag tgtggttttg taaatccagg ctttgtccaa tgtgcaactg gaggagagca4441 atgaaacatg gcattcagtc acaaaaggtt gttgctgaag ttattaaaca aaagccaaca4501 gttcgttggt tgtttctcac attaacagtt aaaaatgttt atgatggcga agaattaaat4561 aagagtttgt cagatatggc tcaaggattt cgccgaatga tgcaatataa aaaaattaat4621 aaaaatcttg ttggttttat gcgtgcaacg gaagtgacaa taaataataa agataattct4681 tataatcagc acatgcatgt attggtatgt gtggaaccaa cttattttaa gaatacagaa4741 aactacgtga atcaaaaaca atggattcaa ttttggaaaa aggcaatgaa attagactat4801 gatccaaatg taaaagttca aatgattcga ccgaaaaata aatataaatc ggatatacaa4861 tcggcaattg acgaaactgc aaaatatcct gtaaaggata cggattttat gaccgatgat4921 gaagaaaaga atttgaaacg tttgtctgat ttggaggaag gtttacaccg taaaaggtta4981 atctcctatg gtggtttgtt aaaagaaata cataaaaaat taaaccttga tgacacagaa5041 gaaggcgatt tgattcatac agatgatgac gaaaaagccg atgaagatgg attttctatt5101 attgcaatgt ggaattggga acggaaaaat tattttatta aagagtagtt caacaaacgg5161 gccagtttgt tgaagattag atgctataat tgttattaaa aggattgaag gatgcttagg5221 aagacgagtt attaatagct gaataagaac ggtgctctcc aaatattctt atttagaaaa5281 gcaaatctaa aattatctga aaagggaatg agaatagtga atggaccaat aataatgact5341 agagaagaaa gaatgaagat tgttcatgaa attaaggaac gaatattgga taaatatggg5401 gatgatgtta aggctattgg tgtttatggc tctcttggtc gtcagactga tgggccctat5461 tcggatattg agatgatgtg tgtcatgtca acagaggaag cagagttcag ccatgaatgg5521 acaaccggtg agtggaaggt ggaagtgaat tttgatagcg aagagattct actagattat5581 gcatctcagg tggaatcaga ttggccgctt acacatggtc aatttttctc tattttgccg5641 atttatgatt caggtggata cttagagaaa gtgtatcaaa ctgctaaatc ggtagaagcc5701 caaacgttcc acgatgcgat ttgtgccctt atcgtagaag agctgtttga atatgcaggc5761 aaatggcgta atattcgtgt gcaaggaccg acaacatttc taccatcctt gactgtacag5821 gtagcaatgg caggtgccat gttgattggt ctgcatcatc gcatctgtta tacgacgagc5881 gcttcggtct taactgaagc agttaagcaa tcagatcttc cttcaggtta tgaccatctg5941 tgccagttcg taatgtctgg tcaactttcc gactctgaga aacttctgga atcgctagag6001 aatttctgga atgggattca ggagtggaca gaacgacacg gatatatagt ggatgtgtca6061 aaacgcatac cattttgaac gatgacctct aataattgtt aatcatgttg gttacgtatt6121 tattaacttc tcctagtatt agtaattatc atggctgtca tggcgcatta acggaataaa6181 gggtgtgctt aaatcgggcc attttgcgta ataagaaaaa ggattaatta tgagcgaatt6241 gaattaataa taaggtaata gatttacatt agaaaatgaa aggggatttt atgcgtgaga6301 atgttacagt ctatcccggc attgccagtc ggggatatta aaaagagtat aggtttttat6361 tgcgataaac taggtttcac tttggttcac catgaagatg gattcgcagt tctaatgtgt6421 aatgaggttc ggattcatct atgggaggca agtgatgaag gctggcgctc tcgtagtaat6481 gattcaccgg tttgtacagg tgcggagtcg tttattgctg gtactgctag ttgccgcatt6541 gaagtagagg gaattgatga attatatcaa catattaagc ctttgggcat tttgcacccc6601 aatacatcat taaaagatca gtggtgggat gaacgagact ttgcagtaat tgatcccgac6661 aacaatttga ttagcttttt tcaacaaata aaaagctaaa atctattatt aatctgttca6721 gcaatcgggc gcgattgctg aataaaagat acgagagacc tctcttgtat cttttttatt6781 ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt tattcttgct6841 gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca agggtttaaa6901 ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc tgatttttaa6961 acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt tttttgtttc7021 ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc ggcactgccg7081 acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata ctttacttgg7141 aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt7201 ta//
