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- 规格：20μl质粒
- 复制子：pUC ori，F1 ori
- 质粒分类：枯草杆菌质粒；枯草游离质粒；枯草胞内质粒
- 质粒大小：7215bp
- 原核抗性：氨苄青霉素Amp
- 5测序引物：PMA0911F：gcgaaaatgcctcacatttgtgcc
- 3测序引物：PMA0911R：cgggatctcagatctggtacgtacc
- 质粒简介： pMA0911是一个枯草杆菌胞内表达质粒。
- 质粒图谱1： png/1-1F9151I550456.png
质粒序列： 点击查看序列LOCUS Exported 7215 bp ds-DNA circular SYN 10-AUG-2017DEFINITION synthetic circular DNASOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 7215)TITLE Direct SubmissionJOURNAL Exported Sep 15, 2017 from MiaoLingPlasmidhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..7215/organism="synthetic DNA construct"/mol_type="other DNA"CDS 4..113/codon_start=1/label=Lip A SP/translation="ILIWSASAAASKRMSMT*ISLLF*FLLNKRKS*HRR"primer_bind complement(185..209)/label=PMA0911Rrep_origin 537..992/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 1018..1122/gene="bla"/label=AmpR promoterCDS 1123..1419/codon_start=1/label=Amp/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIDCNLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSAE"CDS 1466..1984/codon_start=1/label=Amp/translation="MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"rep_origin 2155..2746/direction=RIGHT/label=ori/note="high-copy-number colE1/pMB1/pBR322/pUC origin ofreplication"promoter complement(3516..3618)/label=cat promoter/note="promoter of the E. coli cat gene"CDS 4157..5161/codon_start=1/label=rep/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"CDS 5321..6091/codon_start=1/label=Kana Neo/translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHGQFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTTFLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSDSEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"CDS 6314..6712/codon_start=1/label=bleomycin/translation="MLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRIHLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSLKDQWWDERDFAVIDPDNNLISFFQQIKS"primer_bind 7172..7195/label=PMA0911FORIGIN1 catatcctca tatggtcagc atccgccgca gcttcgaagc gtatgtcgat gacatgaata61 tcattactgt tctgattcct gctgaacaaa aggaaatcat gacaccgccg gaattcgtcg121 acaagcttgg taccagatct gatatccctg cagggatcct ctagagtcga gctcaagcta181 gcttggtacg taccagatct gagatcccgg gttctagagg tcgaaattca cctcgaaagc241 aagctgataa accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc301 aacgtgaaaa aattattatt cgcaattcca agctaattca cctcgaaagc aagctgataa361 accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc aacgtgaaaa421 aattattatt cgcaattcca agctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg cagatcacgc541 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac601 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt661 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc721 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc781 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact841 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg901 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc961 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc1021 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa1081 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc1141 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa1201 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgac1261 tgcaatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg1321 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa1381 gagcaactcg gtcgccgcat acactattct gcagaatgac ttggttgagt actcaccagt1441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac1501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct1561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga1621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac1681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat1741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg1801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc1861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc1921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg1981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta2041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg2101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga2161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt2221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag2281 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa2341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag2401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca2461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac2521 cgaagtactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag2581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct2641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga2701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc2761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt2821 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg2881 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg2941 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc3001 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc3061 accccaggct ttacacttta tgcttccggc atattctcaa taaacccttt agggaaatag3121 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa3181 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg3241 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat3301 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc3361 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctaaacggt ctggttatag3421 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata3481 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat3541 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa3601 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta3661 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat3721 tcgaagacga aagggcatcg cgcgcgggaa attcccggga gagctcgata tcgcatgcgg3781 tacctctaga agaagcttgg agacaaggta aaggataaaa cagcacaatt ccaagaaaaa3841 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa3901 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc3961 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa ataacgtcat4021 ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt4081 gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat aagtgactaa4141 acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata gtagcattta4201 ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag tgagaccatg4261 gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc ttgaatttaa4321 aaaggctgaa agagtaaaag attgtgctga aatattagag tataaacaaa atcgtgaaac4381 aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa4441 ctggaggaga gcaatgaaac atggcattca gtcacaaaag gttgttgctg aagttattaa4501 acaaaagcca acagttcgtt ggttgtttct cacattaaca gttaaaaatg tttatgatgg4561 cgaagaatta aataagagtt tgtcagatat ggctcaagga tttcgccgaa tgatgcaata4621 taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca acggaagtga caataaataa4681 taaagataat tcttataatc agcacatgca tgtattggta tgtgtggaac caacttattt4741 taagaataca gaaaactacg tgaatcaaaa acaatggatt caattttgga aaaaggcaat4801 gaaattagac tatgatccaa atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa4861 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt4921 tatgaccgat gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca4981 ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct5041 tgatgacaca gaagaaggcg atttga
