• 规格：20μl质粒
• 启动子：CMV
• 质粒分类：哺乳细胞慢病毒包装载体
• 质粒大小：8889 bp
• 原核抗性：Amp
• 克隆菌株：Stbl3
• 备注：载体使用Stbl3感受态细胞扩增质粒，可以减少载体基因重组几率。pLP/VSVG， pLP1， pLP2载体是Invitrogen公司出品的配套使用的慢病毒包装载体。 第三代慢病毒包装系统组成成份有pLP1、pLP2和pLP/VSVG（或者pCMV-VSVG）。在使用的时候，按照pLP1(3):pLP2(2):pLP/VSVG(3)的比例配成1 ug/ul的第三代慢病毒包装系统package Mix。转染时，对于60mm的细胞培养皿来说，按照慢病毒基因质粒（3ug）和package mix（6 ug，也就是6 ul）的比例进行慢病毒质粒包装实验。
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质粒序列： 点击查看序列

1 TTGGCCCATT GCATACGTTG TATCCATATC ATAATATGTA CATTTATATT GGCTCATGTC 61 CAACATTACC GCCATGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG 121 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC 181 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA 241 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG 301 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG 361 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT 421 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA 481 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG 541 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT 601 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG 661 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA 721 GAAGACACCG GGACCGATCC AGCCTCCCCT CGAAGCTTAC ATGTGGTACC GAGCTCGGAT 781 CCTGAGAACT TCAGGGTGAG TCTATGGGAC CCTTGATGTT TTCTTTCCCC TTCTTTTCTA 841 TGGTTAAGTT CATGTCATAG GAAGGGGAGA AGTAACAGGG TACACATATT GACCAAATCA 901 GGGTAATTTT GCATTTGTAA TTTTAAAAAA TGCTTTCTTC TTTTAATATA CTTTTTTGTT 961 TATCTTATTT CTAATACTTT CCCTAATCTC TTTCTTTCAG GGCAATAATG ATACAATGTA 1021 TCATGCCTCT TTGCACCATT CTAAAGAATA ACAGTGATAA TTTCTGGGTT AAGGCAATAG 1081 CAATATTTCT GCATATAAAT ATTTCTGCAT ATAAATTGTA ACTGATGTAA GAGGTTTCAT 1141 ATTGCTAATA GCAGCTACAA TCCAGCTACC ATTCTGCTTT TATTTTATGG TTGGGATAAG 1201 GCTGGATTAT TCTGAGTCCA AGCTAGGCCC TTTTGCTAAT CATGTTCATA CCTCTTATCT 1261 TCCTCCCACA GCTCCTGGGC AACGTGCTGG TCTGTGTGCT GGCCCATCAC TTTGGCAAAG 1321 CACGTGAGAT CTGAATTCGA GATCTGCCGC CGCCATGGGT GCGAGAGCGT CAGTATTAAG 1381 CGGGGGAGAA TTAGATCGAT GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA 1441 TAAATTAAAA CATATAGTAT GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG 1501 CCTGTTAGAA ACATCAGAAG GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA 1561 GACAGGATCA GAAGAACTTA GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA 1621 TCAAAGGATA GAGATAAAAG ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA 1681 CAAAAGTAAG AAAAAAGCAC AGCAAGCAGC AGCTGACACA GGACACAGCA ATCAGGTCAG 1741 CCAAAATTAC CCTATAGTGC AGAACATCCA GGGGCAAATG GTACATCAGG CCATATCACC 1801 TAGAACTTTA AATGCATGGG TAAAAGTAGT AGAAGAGAAG GCTTTCAGCC CAGAAGTGAT 1861 ACCCATGTTT TCAGCATTAT CAGAAGGAGC CACCCCACAA GATTTAAACA CCATGCTAAA 1921 CACAGTGGGG GGACATCAAG CAGCCATGCA AATGTTAAAA GAGACCATCA ATGAGGAAGC 1981 TGCAGAATGG GATAGAGTGC ATCCAGTGCA TGCAGGGCCT ATTGCACCAG GCCAGATGAG 2041 AGAACCAAGG GGAAGTGACA TAGCAGGAAC TACTAGTACC CTTCAGGAAC AAATAGGATG 2101 GATGACACAT AATCCACCTA TCCCAGTAGG AGAAATCTAT AAAAGATGGA TAATCCTGGG 2161 ATTAAATAAA ATAGTAAGAA TGTATAGCCC TACCAGCATT CTGGACATAA GACAAGGACC 2221 AAAGGAACCC TTTAGAGACT ATGTAGACCG ATTCTATAAA ACTCTAAGAG CCGAGCAAGC 2281 TTCACAAGAG GTAAAAAATT GGATGACAGA AACCTTGTTG GTCCAAAATG CGAACCCAGA 2341 TTGTAAGACT ATTTTAAAAG CATTGGGACC AGGAGCGACA CTAGAAGAAA TGATGACAGC 2401 ATGTCAGGGA GTGGGGGGAC CCGGCCATAA AGCAAGAGTT TTGGCTGAAG CAATGAGCCA 2461 AGTAACAAAT CCAGCTACCA TAATGATACA GAAAGGCAAT TTTAGGAACC AAAGAAAGAC 2521 TGTTAAGTGT TTCAATTGTG GCAAAGAAGG GCACATAGCC AAAAATTGCA GGGCCCCTAG 2581 GAAAAAGGGC TGTTGGAAAT GTGGAAAGGA AGGACACCAA ATGAAAGATT GTACTGAGAG 2641 ACAGGCTAAT TTTTTAGGGA AGATCTGGCC TTCCCACAAG GGAAGGCCAG GGAATTTTCT 2701 TCAGAGCAGA CCAGAGCCAA CAGCCCCACC AGAAGAGAGC TTCAGGTTTG GGGAAGAGAC 2761 AACAACTCCC TCTCAGAAGC AGGAGCCGAT AGACAAGGAA CTGTATCCTT TAGCTTCCCT 2821 CAGATCACTC TTTGGCAGCG ACCCCTCGTC ACAATAAAGA TAGGGGGGCA ATTAAAGGAA 2881 GCTCTATTAG ATACAGGAGC AGATGATACA GTATTAGAAG AAATGAATTT GCCAGGAAGA 2941 TGGAAACCAA AAATGATAGG GGGAATTGGA GGTTTTATCA AAGTAAGACA GTATGATCAG 3001 ATACTCATAG AAATCTGCGG ACATAAAGCT ATAGGTACAG TATTAGTAGG ACCTACACCT 3061 GTCAACATAA TTGGAAGAAA TCTGTTGACT CAGATTGGCT GCACTTTAAA TTTTCCCATT 3121 AGTCCTATTG AGACTGTACC AGTAAAATTA AAGCCAGGAA TGGATGGCCC AAAAGTTAAA 3181 CAATGGCCAT TGACAGAAGA AAAAATAAAA GCATTAGTAG AAATTTGTAC AGAAATGGAA 3241 AAGGAAGGAA AAATTTCAAA AATTGGGCCT GAAAATCCAT ACAATACTCC AGTATTTGCC 3301 ATAAAGAAAA AAGACAGTAC TAAATGGAGA AAATTAGTAG ATTTCAGAGA ACTTAATAAG 3361 AGAACTCAAG ATTTCTGGGA AGTTCAATTA GGAATACCAC ATCCTGCAGG GTTAAAACAG 3421 AAAAAATCAG TAACAGTACT GGATGTGGGC GATGCATATT TTTCAGTTCC CTTAGATAAA 3481 GACTTCAGGA AGTATACTGC ATTTACCATA CCTAGTATAA ACAATGAGAC ACCAGGGATT 3541 AGATATCAGT ACAATGTGCT TCCACAGGGA TGGAAAGGAT CACCAGCAAT ATTCCAGTGT 3601 AGCATGACAA AAATCTTAGA GCCTTTTAGA AAACAAAATC CAGACATAGT CATCTATCAA 3661 TACATGGATG ATTTGTATGT AGGATCTGAC TTAGAAATAG GGCAGCATAG AACAAAAATA 3721 GAGGAACTGA GACAACATCT GTTGAGGTGG GGATTTACCA CACCAGACAA AAAACATCAG 3781 AAAGAACCTC CATTCCTTTG GATGGGTTAT GAACTCCATC CTGATAAATG GACAGTACAG 3841 CCTATAGTGC TGCCAGAAAA GGACAGCTGG ACTGTCAATG ACATACAGAA ATTAGTGGGA 3901 AAATTGAATT GGGCAAGTCA GATTTATGCA GGGATTAAAG TAAGGCAATT ATGTAAACTT 3961 CTTAGGGGAA CCAAAGCACT AACAGAAGTA GTACCACTAA CAGAAGAAGC AGAGCTAGAA 4021 CTGGCAGAAA ACAGGGAGAT TCTAAAAGAA CCGGTACATG GAGTGTATTA TGACCCATCA 4081 AAAGACTTAA TAGCAGAAAT ACAGAAGCAG GGGCAAGGCC AATGGACATA TCAAATTTAT 4141 CAAGAGCCAT TTAAAAATCT GAAAACAGGA AAGTATGCAA GAATGAAGGG TGCCCACACT 4201 AATGATGTGA AACAATTAAC AGAGGCAGTA CAAAAAATAG CCACAGAAAG CATAGTAATA 4261 TGGGGAAAGA CTCCTAAATT TAAATTACCC ATACAAAAGG AAACATGGGA AGCATGGTGG 4321 ACAGAGTATT GGCAAGCCAC CTGGATTCCT GAGTGGGAGT TTGTCAATAC CCCTCCCTTA 4381 GTGAAGTTAT GGTACCAGTT AGAGAAAGAA CCCATAATAG GAGCAGAAAC TTTCTATGTA 4441 GATGGGGCAG CCAATAGGGA AACTAAATTA GGAAAAGCAG GATATGTAAC TGACAGAGGA 4501 AGACAAAAAG TTGTCCCCCT AACGGACACA ACAAATCAGA AGACTGAGTT ACAAGCAATT 4561 CATCTAGCTT TGCAGGATTC GGGATTAGAA GTAAACATAG TGACAGACTC ACAATATGCA 4621 TTGGGAATCA TTCAAGCACA ACCAGATAAG AGTGAATCAG AGTTAGTCAG TCAAATAATA 4681 GAGCAGTTAA TAAAAAAGGA AAAAGTCTAC CTGGCATGGG TACCAGCACA CAAAGGAATT 4741 GGAGGAAATG AACAAGTAGA TAAATTGGTC AGTGCTGGAA TCAGGAAAGT ACTATTTTTA 4801 GATGGAATAG ATAAGGCCCA AGAAGAACAT GAGAAATATC ACAGTAATTG GAGAGCAATG 4861 GCTAGTGATT TTAACCTACC ACCTGTAGTA GCAAAAGAAA TAGTAGCCAG CTGTGATAAA 4921 TGTCAGCTAA AAGGGGAAGC CATGCATGGA CAAGTAGACT GTAGCCCAGG AATATGGCAG 4981 CTAGATTGTA CACATTTAGA AGGAAAAGTT ATCTTGGTAG CAGTTCATGT AGCCAGTGGA 5041 TATATAGAAG CAGAAGTAAT TCCAGCAGAG ACAGGGCAAG AAACAGCATA CTTCCTCTTA 5101 AAATTAGCAG GAAGATGGCC AGTAAAAACA GTACATACAG ACAATGGCAG CAATTTCACC 5161 AGTACTACAG TTAAGGCCGC CTGTTGGTGG GCGGGGATCA AGCAGGAATT TGGCATTCCC 5221 TACAATCCCC AAAGTCAAGG AGTAATAGAA TCTATGAATA AAGAATTAAA GAAAATTATA 5281 GGACAGGTAA GAGATCAGGC TGAACATCTT AAGACAGCAG TACAAATGGC AGTATTCATC 5341 CACAATTTTA AAAGAAAAGG GGGGATTGGG GGGTACAGTG CAGGGGAAAG AATAGTAGAC 5401 ATAATAGCAA CAGACATACA AACTAAAGAA TTACAAAAAC AAATTACAAA AATTCAAAAT 5461 TTTCGGGTTT ATTACAGGGA CAGCAGAGAT CCAGTTTGGA AAGGACCAGC AAAGCTCCTC 5521 TGGAAAGGTG AAGGGGCAGT AGTAATACAA GATAATAGTG ACATAAAAGT AGTGCCAAGA 5581 AGAAAAGCAA AGATCATCAG GGATTATGGA AAACAGATGG CAGGTGATGA TTGTGTGGCA 5641 AGTAGACAGG ATGAGGATTA ACACATGGAA TTCCGGAGCG GCCGCAGGAG CTTTGTTCCT 5701 TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA 5761 GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA 5821 GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT 5881 CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG 5941 AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA 6001 ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG 6061 CTTCCGCGGA ATTCACCCCA CCAGTGCAGG CTGCCTATCA GAAAGTGGTG GCTGGTGTGG 6121 CTAATGCCCT GGCCCACAAG TATCACTAAG CTCGCTTTCT TGCTGTCCAA TTTCTATTAA 6181 AGGTTCCTTT GTTCCCTAAG TCCAACTACT AAACTGGGGG ATATTATGAA GGGCCTTGAG 6241 CATCTGGATT CTGCCTAATA AAAAACATTT ATTTTCATTG CAATGATGTA TTTAAATTAT 6301 TTCTGAATAT TTTACTAAAA AGGGAATGTG GGAGGTCAGT GCATTTAAAA CATAAAGAAA 6361 TGAAGAGCTA GTTCAAACCT TGGGAAAATA CACTATATCT TAAACTCCAT GAAAGAAGGT 6421 GAGGCTGCAA ACAGCTAATG CACATTGGCA ACAGCCCCTG ATGCCTATGC CTTATTCATC 6481 CCTCAGAAAA GGATTCAAGT AGAGGCTTGA TTTGGAGGTT AAAGTTTTGC TATGCTGTAT 6541 TTTACATTAC TTATTGTTTT AGCTGTCCTC ATGAATGTCT TTTCACTACC CATTTGCTTA 6601 TCCTGCATCT CTCAGCCTTG ACTCCACTCA GTTCTCTTGC TTAGAGATAC CACCTTTCCC 6661 CTGAAGTGTT CCTTCCATGT TTTACGGCGA GATGGTTTCT CCTCGCCTGG CCACTCAGCC 6721 TTAGTTGTCT CTGTTGTCTT ATAGAGGTCT ACTTGAAGAA GGAAAAACAG GGGGCATGGT 6781 TTGACTGTCC TGTGAGCCCT TCTTCCCTGC CTCCCCCACT CACAGTGACC CGGAATCCCT 6841 CGACATGGCA GTCTAGCACT AGTGCGGCCG CAGATCTGCT TCCTCGCTCA CTGACTCGCT 6901 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 6961 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 7021 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 7081 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 7141 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 7201 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 7261 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 7321 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 7381 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 7441 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 7501 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 7561 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 7621 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 7681 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 7741 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 7801 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 7861 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 7921 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 7981 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 8041 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 8101 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 8161 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 8221 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 8281 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 8341 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 8401 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 8461 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 8521 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 8581 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 8641 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 8701 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 8761 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGGGA TCCCCTGAGG 8821 GGGCCCCCAT GGGCTAGAGG ATCCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 8881 GCCATGAGC