- 规格:20μl质粒
- 启动子:CMV
- 质粒分类:哺乳动物细胞慢病毒表达载体,shRNA,RNA干扰载体
- 质粒大小:7758 bp
- 原核抗性:Amp
- 筛选标记:Puro
- 克隆菌株:Stbl3
- 备注:含有组成型人U6启动子的慢病毒shRNA载体
质粒序列: 点击查看序列1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatctgggc aggaagaggg cctatttccc atgattcctt 2221 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa 2281 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg 2341 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt 2401 tcgatttctt ggctttatat atcttgtgga aaggacgagg atccggacaa gcttcgaatt 2461 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 2521 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 2581 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 2641 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 2701 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 2761 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 2821 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 2881 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 2941 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag 3001 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 3061 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 3121 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 3181 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 3241 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 3301 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 3361 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 3421 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 3481 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 3541 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac 3601 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggcgtctcg agtctagagg 3661 gcccgacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 3721 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 3781 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 3841 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 3901 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 3961 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 4021 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 4081 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 4141 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 4201 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 4261 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta 4321 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg 4381 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag 4441 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa 4501 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga 4561 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag 4621 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac 4681 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg 4741 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact 4801 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc 4861 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc 4921 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 4981 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 5041 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 5101 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 5161 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc 5221 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 5281 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 5341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 5401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 5461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 5521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 5581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 5641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 5701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 5761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 5821 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 5881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 5941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 6001 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 6061 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 6121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 6181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 6241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 6301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 6361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 6421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 6481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 6541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 6601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 6661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 6721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 6781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 6841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 6901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 6961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 7021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 7081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 7141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 7201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 7261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 7321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 7381 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa 7441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 7501 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc 7561 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg 7621 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat 7681 ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt 7741 actacaaact tagtagt //