• 规格：20μl质粒
• 质粒分类：枯草整合分泌表达质粒
• 质粒大小：8717 bp
• 原核抗性：Amp
• 克隆菌株：DH5α

质粒序列： 点击查看序列

1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga 61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt 121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat 181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt 241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg 301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg 361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg 421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa 481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat 541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca 601 catggaaaca cacaaattaa aaactggtct gatcggatcc gcggctagcc atggtcgacg 661 cggccgcaaa attgaaaaaa tggtggaaac acttttttca atttttttgt tttattattt 721 aatatttggg aaatattcat tctaattggt aatcagattt tagaaaacaa taaacccttg 781 cataggggga tcggatccta actcacatta attgcgttgc gctcactgcc cgctttccag 841 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 901 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt 961 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag 1021 caggcgaaaa tcctgtttga tggtggttga cggcgggata taacatgagc tgtcttcggt 1081 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc 1141 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc 1201 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg 1261 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag 1321 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc 1381 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat 1441 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac 1501 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc 1561 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac 1621 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg 1681 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag 1741 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc 1801 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga 1861 gacaccggca tactctgcga catcgtataa cgttactggt ttcatcaaaa tcgtctccct 1921 ccgtttgaat atttgattga tcgtaaccag atgaagcact ctttccacta tccctacagt 1981 gttatggctt gaacaatcac gaaacaataa ttggtacgta cgatctttca gccgactcaa 2041 acatcaaatc ttacaaatgt agtctttgaa agtattacat atgtaagatt taaatgcaac 2101 cgttttttcg gaaggaaatg atgacctcgt ttccaccgga attagcttgc atgcctaccc 2161 aatcagtacg ttaattttgg ctttaatgag ttgtaattcc tctggggcaa ctgttccttt 2221 cttcgttgct cctcttgccc gccaggcgat actttttacc tgatcagcta acgctacgcc 2281 atcacgttcc tgaccggata aaacaacttc gaacggatat ccttttgatt gcgttgtaca 2341 aggaacacac agacacatac ctgttttgtt gttgtacatg aaaggactca ggacaacagc 2401 tggacgatgt ccagcttgct cgctaccttt tgtcgggtca aaatcaaccc aaatcagatc 2461 gcccatatcg ggtacgtatc ggcttaccat acctgcttcc tccttaagct taattgttat 2521 ccgctcacaa ttccacacat tatgccacac cttgtagata aagtcaacaa ctttttgcaa 2581 aatttttcag gaattttagc agaggttgtt ctggatgtag aacaaaacat ctttccgctc 2641 ttgtgctgtt aggatatctt tcttggaagc taggtaggca agggctacct caaataaatc 2701 ttcttcaggg tgcgctattt ttaaggtgcc tagtgaggtc ttgaccactt cacccataat 2761 ttcagtgccg aatagtctgg actgggctgt gtagaattcc tgcagccctg gcgaatggcg 2821 attttcgttc gtgaatacat gttataataa ctataactaa taacgtaacg tgactggcaa 2881 gagatatttt taaaacaatg aataggttta cacttacttt agttttatgg aaatgaaaga 2941 tcatatcata tataatctag aataaaatta actaaaataa ttattatcta gataaaaaat 3001 ttagaagcca atgaaatcta taaataaact aaattaagtt tatttaatta acaactatgg 3061 atataaaata ggtactaatc aaaatagtga ggaggatata tttgaataca tacgaacaaa 3121 ttaataaagt gaaaaaaata cttcggaaac atttaaaaaa taaccttatt ggtacttaca 3181 tgtttggatc aggagttgag agtggactaa aaccaaatag tgatcttgac tttttagtcg 3241 tcgtatctga accattgaca gatcaaagta aagaaatact tatacaaaaa attagaccta 3301 tttcaaaaaa aataggagat aaaagcaact tacgatatat tgaattaaca attattattc 3361 agcaagaaat ggtaccgtgg aatcatcctc ccaaacaaga atttatttat ggagaatggt 3421 tacaagagct ttatgaacaa ggatacattc ctcagaagga attaaattca gatttaacca 3481 taatgcttta ccaagcaaaa cgaaaaaata aaagaatata cggaaattat gacttagagg 3541 aattactacc tgatattcca ttttctgatg tgagaagagc cattatggat tcgtcagagg 3601 aattaataga taattatcag gatgatgaaa ccaactctat attaacttta tgccgtatga 3661 ttttaactat ggacacgggt aaaatcatac caaaagatat tgcgggaaat gcagtggctg 3721 aatcttctcc attagaacat agggagagaa ttttgttagc agttcgtagt tatcttggag 3781 agaatattga atggactaat gaaaatgtaa atttaactat aaactattta aataacagat 3841 taaaaaaatt ataaaaaaat tgaaaaaatg gtggaaacac ttttttcaat ttttttgttt 3901 tattatttaa tatttgggaa atattcattc taattggtaa tcagatttta gaaaacaata 3961 aacccttgca tagggggatc tcgacatgga tgagcgatga tgatatccgt ttaggctggg 4021 cggtgatagc ttctcgttca ggcagtacgc ctcttttctt ttccagacct gagggaggcg 4081 gaaatggtgt gaggttcccg gggaaaagcc aaataggcga tcgcgggagt gctttatttg 4141 aagatcaggc tatcactgcg gtcaatagat ttcacaatgt gatggctgga cagcctgagg 4201 aactctcgaa cccgaatgga aacaaccaga tatttatgaa tcagcgcggc tcacatggcg 4261 ttgtgctggc aaatgcaggt tcatcctctg tctctatcaa tacggcaaca aaattgcctg 4321 atggcaggta tgacaataaa gctggagcgg gttcatttca agtgaacgat ggtaaactga 4381 caggcacgat caatgccagg tctgtagctg tgctttatcc tgatgatatt gcaaaagcgc 4441 ctcatgtttt ccttgagaat tacaaaacag gtgtaacaca ttctttcaat gatcaactga 4501 cgattacctt gcgtgcagat gcgaatacaa caaaagccgt ttatcaaatc aataatggac 4561 cagacgacag gcgtttaagg atggagatca attcacaatc ggaaaaggag atccaatttg 4621 gcaaaacata caccatcatg ttaaaaggaa cgaacagtga tggtgtaacg aggaccgaga 4681 aatacagttt tgttaaaaga gatccagcgt cggccaaaac catcggctat caaaatccga 4741 atcattggag ccaggtaaat gcttatatct ataaacatga tgggagccga gtaattgaat 4801 tgaccggatc ttggcctgga aaaccaatga ctaaaaatgc agacggaatt tacacgctga 4861 cgctgcctgc ggacacggat acaaccaacg caaaagtgat ttttaataat ggcagcgccc 4921 aagtgcccgg tcagaatcag cctggctttg attacgtgct aaatggttta tataatgact 4981 cgggcttaag cggttctctt ccccattgag ggcaaggcta gacgggactt accgaaagaa 5041 accatcaatg atggtttctt ttttgttcat aaatcagaca aaacttttct cttgcaaaag 5101 tttgtgaagt gttgcacaat ataaatgtga aatacttcac aaacaaaaag acatcaaaga 5161 gaaacatacc ctgcaaggat gctgatattg tctgcatttg cgccggagca aaccaaaaac 5221 ctggtgagac acgccttgaa ttagtagaaa agaacttgaa gattttcaaa ggcatcgtta 5281 gtgaagtcat ggcgagcgga tttgacggca ttttcttagt cgcgacgcga ggctggatgg 5341 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca 5401 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc 5461 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg 5521 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc 5581 ccgcgttgcg tcgcggtgca tggagccggg ccacctactg aagtggattt ctttaagagc 5641 tccgataacc agaagcaata aaaaatcaaa tcggatttca ctatataatc tcactttatc 5701 taagatgaat ccgatggaag catcctgttt tctctcaatt tttttatcta aaacccagcg 5761 ttcgatgctt ctttgagcga acgatcaaaa ataagtgcct tcccatcaaa aaaatattct 5821 caacataaaa aactttgtgt aatacttgta acgctacatg gagattaact caatctagag 5881 ggtattaata atgatccaca gtagcgtaaa gcgttgggga aattcaccgg cggtgcggat 5941 cccggctacg ttaatgcagg cgctcaatct gaatattgat gatgaagtga agattgacct 6001 ggtggatggc aaattaatta ttgagccagt gcgtaaagag cccgtattta cgcttgctga 6061 actggtcaac gacatcacgc cggaaaacct ccacgagaat atcgactggg gagagccgaa 6121 agataaggaa gtctggtaaa tcgattatgt cttttgcgca gtcggcttaa accagttttc 6181 gctggtgcga aaaaagagtg tcttgtgaca cctaaattca aaatctatcg gtcagattta 6241 taccgatttg attttatata ttcttgaata acatacgccg agttatcaca taaaagcggg 6301 aaccaatcat caaatttaaa cttcattgca taatccatta aactcttaaa ttctacgatt 6361 ccttgttcat caataaactc aatcatttct ttaattaatt tatatctatc tgttgttgtt 6421 ttctttaata attcatcaac atctacaccg ccataaacta tcatatcttc tttttgatat 6481 ttaaatttat taggatctta aggcctaggt ctagagtctt tgttttgacg ccattagcgt 6541 acgtaacaat cctcgttaaa ggacaaggac ctgagcggaa gtgtatcgta cagtagacgg 6601 agtatactag tatagtctat agtccgtgga attattatat ttatctccga cgatattctc 6661 atcagtgaaa tccagctgga gttctttagc aaattttttt attagctgaa cttagtatta 6721 gtggccatac tcctccaatc caaagctatt tagaaagatt actatatcct caaacaggcg 6781 gtaaccggcc tcttcatcgg gaatgcgcgc gaccttcagc atcgccggca tgtccccctg 6841 gcggacggga agtatccagc tcgaggtcgg gccgcgttgc tggcgttttt ccataggctc 6901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 6961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 7021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 7081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 7141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 7201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 7261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 7321 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 7381 gttgatagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 7441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 7501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 7561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 7621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 7681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 7741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 7801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 7861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 7921 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca 7981 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 8041 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 8101 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 8161 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 8221 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 8281 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 8341 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 8401 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 8461 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 8521 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 8581 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 8641 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 8701 tttcgtcttc aagaatt