×
![]()
- 规格：20μl质粒
- 复制子：ColE1 Ori
- 质粒分类：枯草杆菌质粒；枯草整合质粒；枯草分泌质粒
- 质粒大小：8965bp
- 原核抗性：氨苄青霉素Amp
- 克隆菌株：DH5α等大肠杆菌
- 质粒简介： pIEFBPR质粒是一个枯草整合型分泌载体，该质粒的参考文献：mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis ColE1 ori, ColE1 replication origin; amp, ampicillin-resistance marker; spc, spectinomycin-resistance marker; lacI, lac-repressor-encoding gene; Pspac, Pspac promoter; mazF, E.coli toxin gene; Plpp, promoter of E.coli lpp gene; mazE, E.coli antitoxin gene; DR, directly-repeated sequence; amyE-front and amyE-back, upstream and downstream sequences of the B.subtilis amyE gene, respectively; bpr-front and bpr-back, upstream and downstream sequences of the B.subtilis bpr gene, respectively. All of the DNA sequences located between the amyE-front and amyE-back or the bpr-front and bpr-back allow integration into the chromosome of B.subtilis via a double-crossover event, resulting in selection for spectinomycin-resistance. The unique restriction enzyme sites are highlighted in blue.
- 质粒图谱1： png/1-1F9151G53C07.png
