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发货规格 |
1×10⁶ |
别名 |
HCT-116; HCT.116; HCT_116; HCT116; HCT116wt; CoCL2 |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
DMEM+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
25~40h |
传代比例 |
1:3~1:4 |
换液频率 |
每周2~3次 |
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种属 |
人源 |
组织来源 |
结肠 |
肿瘤类型 |
肠癌 |
供体信息 |
成年男性 |
背景描述 |
HCT 116 细胞系是从患有结肠癌的成年男性的结肠中分离出来的。它在 ras 原癌基因的密码子 13 中发生突变,可用作该密码子突变的 PCR 测定的阳性对照 |
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同工酶 |
AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1 PGM1, 1 PGM3, 1 |
基因表达 |
carcinoembryonic antigen (CEA) 1 ng per 106 cells per 10 days |
致瘤性 |
Yes, in nude mice |
病毒 |
PCR: EBV -, HBV -, HCV -, HIV -, HTLV-1/2 -, MLV -, SMRV - |
核型 |
The stemline chromosome number is near diploid with the modal number at 45 (62%) and polyploids occurring at 6.8%. The markers 10q+ and t(?8p;18q) are present in all metaphases and t(9q;?16p-), in 80% of the cells karyotyped. N16 is monosomic in the presence of, but disomic in the absence of t(9q;?16p-). N10 and N18 are monosomic and other chromosomes from those mentioned above are disomic. Q-band observations revealed the presence of the Y chromosome, but not in all cells (50% of cells lacked the Y in G-band karyotypes). |