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发货规格 |
1×10⁶ |
别名 |
SW-480; SW 480; SW480E |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
L-15+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
30~50h |
传代比例 |
1:2~1:4 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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种属 |
人源 |
组织来源 |
大肠 |
肿瘤类型 |
腺癌 |
供体信息 |
50岁白人女性 |
背景描述 |
SW480 [SW-480] 细胞从 Dukes C 结直肠癌患者的大肠中分离出来,可用于癌症研究。 |
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蛋白表达 |
The cells express elevated levels of p53 protein. |
同工酶 |
G6PD, B, PGM1, 2, PGM3, 1, 6PGD, A, PEP-D, 1, ES-D, 1 |
基因表达 |
carcinoembryonic antigen (CEA) 0.7 ng/106 cells/10 days; keratin; transforming growth factor beta; myc+; myb+ ; ras+; fos+; sis+; p53+; abl -; ros -; src -; HLA: (A2; B8; B17); blood type A; Rh+; keratin positive by immunoperoxidase staining; c-myc; K-ras; H-ras; N-ras; myb; sis; fos |
致癌基因 |
myc+; myb+; ras+; fos+; sis+; p53+; abl-; ros-; src- |
致瘤性 |
Yes, in nude mice |
抗原表达 |
HLA A2, B8, B17, blood type A, Rh+. The line is negative for CSAp (CSAp-) and colon antigen 3 |
病毒 |
Reverse transcriptase negative |
病毒易感性 |
Human immunodeficiency virus 1 |
核型 |
The stemline chromosome number is hypotriploid and 11-12 marker chromosomes were common. Both double minutes and dicentrics were observed in 8% of each metaphase examined. |